Method Validation for Analysis of Simvastatin in Human Plasma Using Liquid Chromatography Tandem Mass Spectrometry (LC-MS-MS)
2739-2743
Correspondence
Dr. khaled M. Alakhali,
Post box no:1882, Postal code: 61441, Abha, Kingdom of Saudi Arabia.
Phone: 00966558649740, E-mail: Khaled_akhali@yahoo.com
Introduction:The Liquid Chromatography Tandem Mass Spectrometry (LC-MS-MS) for determination simvastatin in human plasma has been developed after extraction by by ethyl acetate and hexane (90/10%, v/v) using lovastatin as internal standard.
Material and Methods:The mobile phase consisting of mixture of acetonitrile and water (75/25%, v/v) 500µL/min by separated the solutes on a C18 column.
Discussion:The lower limit of quantitation of 0.25 ng/mL was achieved when the calibration curve was linear from 0.25-50 ng/mL. The entire run time for analysis was only 6 min. The quantitation in the selective reaction monitoring (SRM) in positive ion mode, the daughter ions m/z 325 for simvastatin and m/z 285 for lovastatin were used. The Parent ions in positive ion mode were m/z 441.3 for simvastatin and m/z 405.1 for lovastatin. The intra-day coefficients of variation were less than 14% while the inter-day coefficients of variation were less than 10%.
Conclusion:The LC-MS-MS detection is sensitive due to its capability to eliminate interferences from endogenous components.