Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Original article / research
Table of Contents - Year : 2017 | Month : August | Volume : 11 | Issue : 8 | Page : PC04 - PC07

An Attempt to Make Platelet Rich Fibrin from Bone Marrow Aspirate PC04-PC07

Soh Nishimoto, Kazutoshi Fujita, Toshihiro Fujiwara, Yohei Sotsuka, Maki Tonooka, Hisako Ishise, Kenichiro Kawai, Masao Kakibuchi

Correspondence
Dr. Soh Nishimoto,
1-1 Mukogawa-cho, Nishinomiya-663-8501, Hyogo, Japan.
E-mail: nishimot@hyomed.ac.jp

Introduction: Platelet Rich Fibrin (PRF) was developed as a new generation of platelet concentration from peripheral blood. Method to make PRF is simple. It is easy to handle with its moderate firmness. Histologically, platelets and nucleated cells are packed along the yellow-red border. Bone marrow aspirate contains bone marrow cells that potentially work for tissue regeneration, and platelets which contain growth factors. The specific gravities of them are comparable. It implies that, if it is possible to make PRF from bone marrow aspirate, then high concentration of platelets and bone marrow cells can be obtained simultaneously by taking out yellow-red interface of it.

Aim: To find out a method to make PRF from bone marrow aspirate.

Materials and Methods: Iliac crest of rabbits were punctured and aspirated with or without anti-coagulant, under general anaesthesia. The bone marrow aspirate was centrifuged in glass tubes. For the bone marrow aspirate taken with anti-coagulant, calcium chloride was added just before centrifugation. Products were taken out and observed grossly. The products were fixed with formaldehyde and observed histologically.

Results: Coagulated gels with two-toned colour were obtained by all methods. In the gels without anti-coagulant, interfaces between two colours were obscure. Histologically, platelets and nucleated cells scattered as clusters. Filtering caused haemolysis and reduced the yield of the product. With the aspirate taken with anti-coagulant, platelets and nucleated cells formed a band along the interface.

Conclusion: PRF can be made from bone marrow aspirate by adding anti-coagulant in aspiration and reversed with calcium chloride just before centrifugation.