VCAM-1(rs3783605A>G) Single-Nucleotide Polymorphism Genotyping in a Sample of Type 2 Diabetes Mellitus Iraqi Patients GC01-GC05
Dr. Nadia Fadhil Salman,
Um Salama Street-665/6/28, Baghdad, Al Gazalia, Iraq.
Introduction: The Vascular Adhesion Molecule-1 (VCAM-1) is a cell surface glycoprotein expressed by cytokine-activated endothelium, mediates the adhesion of monocytes and lymphocytes. Diabetes mellitus is a common global health problem represented by metabolic disorder in which common genetic variants interact with environmental factors like older age, obesity, family history of diabetes to unmask the disease which might be a result of inflammation reflecting response.
Aim: Detection of the VCAM-1(rs3783605 A>G) gene polymorphism in a group of Type 2 Diabetes Mellitus (T2DM) Iraqi patients.
Materials and Methods: The study enrolled 129 subjects (68 patients, 61 controls). Clinical features and laboratory investigations included: Body Mass Index, Abdominal Circumference, fasting blood sugar, Glycohemoglobin (HbA1c) and lipid profile were measured for each subject. Genotyping of the VCAM-1(rs3783605 A>G) gene polymorphism was performed using Sequence Specific Primer (SSP) Polymerase Chain Reaction (PCR) technique and the PCR product was visualized by agarose gel electrophoresis.
Results: The clinical characteristics showed a significant increase (p<0.05) in the patients group compared to the control group. VCAM-1polymorphism analysis showed a higher frequency of A/G genotype in control group than patients group (100% versus 88.2%), while the analysis of the allele impact, including the genotypes that contain the G allele showed no significant association in patient group compared with control.
Conclusion: The results of the present study showed that control group had a higher frequency of the A/G genotype regarding the patients group, and that the G allele of the VCAM-1 (rs3783605 A>G) single nucleotide polymorphism has more role in the pathogenesis of T2DM than the A allele, and could be considered as a risk factor for T2DM in Iraqi population, as well as the association of the A/G genotype with the incidence of T2DM in Iraqi patients.