Free Radicals and Antioxidant Status in Chronic Osteomyelitis Patients: A Case Control Study
Correspondence Address :
Dr. Surendra Pratap Mishra,
Assistant Professor, Department of Biochemistry, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
E-mail: drsurendram2@gmail.com
Introduction: Osteomyelitis (OM) is a local or generalized infection of the bone and bone marrow which may be multifactorial in its causation. Chronic infection is characterised by sequestrum and involucrum formation.
Aim: The present study has been carried out for assessing the oxidative stress in chronic OM by measurement of serum oxidants {such as malondialdehyde (MDA), protein carbonyl (PC), nitrite} and the serum antioxidants {such as ascorbic acid, superoxide dismutase (SOD), ceruloplasmin (Cp), blood glutathione} by spectrophotometric method.
Materials and Methods: This was a case control study. About 5 ml of venous blood was collected for the estimation of biochemical parameters. This study comprised of 50 OM patients diagnosed at SSLH Hospital, Varanasi and 50 healthy ages (15-35 y) and sex matched individuals.
Results: Significantly increased (p<0.0001) levels of serum oxidants and significantly decreased (p<0.0001) levels of all serum antioxidants except ceruloplasmin indicated significantly increased (p<0.0001) levels in response to infections in chronic OM patients as compared to the healthy controls.
Conclusion: These results suggest that there occurs an imbalance between oxidants and antioxidants, especially an increase in oxidative stress, as measured by the levels of the parameters: serum MDA, serum protein carbonyl and serum nitrite.
Ceruloplasmin, Malondialdehyde, Nitric oxide synthase, Nitric oxide, Protein carbonyl, Superoxide dismutase
DOI: 10.7860/JCDR/2015/11950.5781
Date of Submission: Nov 02, 2014
Date of Peer Review: Feb 02, 2015
Date of Acceptance: Feb 24, 2015
Date of Publishing: Apr 01, 2015
Financial OR OTHER COMPETING INTERESTS: None.
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