Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Original article / research
Year : 2017 | Month : April | Volume : 11 | Issue : 4 | Page : DC01 - DC03

Auxanographic Carbohydrate Assimilation Method for Large Scale Yeast Identification

Suganthi Martena Devadas, Mamatha Ballal, Peralam Yegneswaran Prakash, Manjunath H Hande, Geetha V Bhat, Vinitha Mohandas

1. Research Scholar, Department of Enteric Diseases Division, KMC, Manipal University, Manipal, Karnataka, India. 2. Professor, Department of Enteric Diseases Division, KMC, Manipal University, Manipal, Karnataka, India. 3. Associate Professor and Incharge-Medical Mycology Laboratory, Department of Microbiology, KMC, Manipal University, Manipal, Karnataka, India. 4. Professor and Head, Department of Medicine, KMC, Manipal University, Manipal, Karnataka, India. 5. Microbiologist and Infection Control Officer, Department of Microbiology, Bhagwan Mahaveer Jain Hospital, Bengaluru, Karnataka, India. 6. Professor, Department of Microbiology, Bangalore Institute of Dental Sciences, Bengaluru, Karnataka, India.

Correspondence Address :
Dr. Mamatha Ballal,
Professor, Department of Microbiology, Enteric Diseases Division – Incharge, Central Research Lab,
Kasturba Medical College - Manipal, Manipal University, Madhav Nagar, Manipal-576104, Karnataka, India.
E-mail: mamatha.ballal08@gmail.com

Abstract

Introduction: The auxanographic carbohydrate assimilation had been an important method for differentiation of yeasts. Prevailing methods described in the literature for carbohydrate assimilation has limited scope for use in large scale yeast identification.

Aim: To optimize the large scale auxanographic carbohydrate assimilation method for yeast identification.

Materials and Methods: A modified auxanographic carbohydrate assimilation method was developed and a total of 35 isolates of Candida species comprising of four ATCC (American Type Culture Collection) Candida strains (Candida albicans ATCC 90028, Candida tropicalis ATCC 90018, Candida parapsilosis ATCC 750, Candida krusei ATCC 6258) and 31 clinical isolates of Candida tropicalis (n=13), Candida krusei (n=7), Candida glabrata (n=3), Candida kefyr (n=3), Candida albicans (n=5) were validated. The carbohydrates tested were Glucose, Sucrose, Maltose, Lactose, Cellubiose, Raffinose, Trehalose, Xylose, Galactose and Dulcitol.

Results: A total of 35 Candida species were tested for their carbohydrate assimilative property and the results were consistent with the existing standard protocols. A well circumscribed opaque yeast growth indicated assimilation of the test carbohydrate and translucent to opalescent growth with the outline of initial inoculum alone indicated lack of assimilation. The control plate indicated no growth of the Candida species.

Conclusion: The carbohydrate assimilation tests finds utility for yeast diversity studies exploring novel ecological niches. The technique described here facilitates testing of an extended range of carbohydrates and yeasts in a cost effective manner.

Keywords

Candida, Control plate, Ecological niche

How to cite this article :

Suganthi Martena Devadas, Mamatha Ballal, Peralam Yegneswaran Prakash, Manjunath H Hande, Geetha V Bhat, Vinitha Mohandas. AUXANOGRAPHIC CARBOHYDRATE ASSIMILATION METHOD FOR LARGE SCALE YEAST IDENTIFICATION. Journal of Clinical and Diagnostic Research [serial online] 2017 April [cited: 2017 May 29 ]; 11:DC01-DC03. Available from
http://jcdr.net/back_issues.asp?issn=0973-709x&year=2017&month=April&volume=11&issue=4&page=DC01-DC03&id=9653

DOI and Others

DOI: 10.7860/JCDR/2017/25967.9653


Date of Submission: Dec 12, 2016
Date of Peer Review: Jan 18, 2017
Date of Acceptance: Feb 01, 2017
Date of Publishing: Apr 01, 2017


Financial OR OTHER COMPETING INTERESTS: None.

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