Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Original article / research
Year : 2017 | Month : April | Volume : 11 | Issue : 4 | Page : DC04 - DC07

Bacterial Contamination of Adult House Flies (Musca domestica) and Sensitivity of these Bacteria to Various Antibiotics, Captured from Hamadan City, Iran

Mansour Nazari, Tahereh Mahrabi, Seyed Mostafa Hosseini, Mohammad Yousef Alikhani

1. Professor, Department of Medical Entomology, Hamadan University of Medical Science, Hamadan, Iran. 2. M.Sc Student, Department of Medical Entomology, Hamadan University of Medical Science, Hamadan, Iran. 3. Ph.D Student, Department of Medical Microbiology, Hamadan University of Medical Science, Hamadan, Iran. 4. Professor, Department of Medical Microbiology, Hamadan University of Medical Science, Hamadan, Iran.

Correspondence Address :
Dr. Mohammad Yousef Alikhani,
Professor, Department of Medical Microbiology, Hamadan University of Medical Science, Hamadan, Iran.
E-mail: alikhani43@yahoo.com

Abstract

Introduction: House flies (Musca domestica) have been known as a mechanical vector in spreading infectious diseases such as cholera, shigellosis, salmonellosis and skin infections.

Aim: To investigate the bacterial contaminations of house flies and determine the resistance of these bacteria against various antibiotics.

Materials and Methods: An analytical descriptive crosssectional study was conducted. The study was performed from July to September 2015 in Hamadan University of Medical Sciences, Hamadan, Iran. A total number of 300 house flies were collected from four places, 75 flies from each place, including two educational hospitals belonging to Hamadan University of Medical Sciences, a fruit and vegetables center, and a livestock slaughter. The body surface of house flies was washed using the physiological sterile serum and the obtained solution was centrifuged at 2000 rpm for five minutes. The identification of bacteria was carried out using the phenotypic methods. The resistance of bacteria against various antibiotics was determined using the disk diffusion approach. Data were analysed by the employment of SPSS software package version 20.0.

Results: A total number of 394 bacterial strains were isolated from 275 house flies. The most prevalent type of bacteria was Bacillus spp which was detected in 31.1% of house flies. Moreover, Staphylococcus spp. (22.9%), Escherichia coli (11.6%) were other prevalent species, whereas, Enterococcus spp. was the least prevalent type of bacteria in the collected house flies. In terms of resistance to antibiotics, it was identified that bacteria extracted from house flies which were collected from hospital environments were more resistant to antibiotics compared with the resistance of bacteria extracted from house flies which were collected from non hospital environments. The maximum bacterial isolation was found in houseflies from hospital No.1 environment from around the accumulation of garbage.

Conclusion: It is a well-known fact that house flies are a source of bacterial contamination and can act as a mechanical carrier and cause bacterial diseases. It can be postulated that house flies play a major role in spreading antibiotic resistant bacteria. However, the flies from hospital environments were more contaminated, mainly because the people referring to health center are normally ill and carrier of many pathogens. Further, hospital environments should be controlled using administrative procedures.

Keywords

Kirby bauer, Staphylococcus aureus, Tetracycline

How to cite this article :

Mansour Nazari, Tahereh Mahrabi, Seyed Mostafa Hosseini, Mohammad Yousef Alikhani. BACTERIAL CONTAMINATION OF ADULT HOUSE FLIES (MUSCA DOMESTICA) AND SENSITIVITY OF THESE BACTERIA TO VARIOUS ANTIBIOTICS, CAPTURED FROM HAMADAN CITY, IRAN. Journal of Clinical and Diagnostic Research [serial online] 2017 April [cited: 2017 May 29 ]; 11:DC04-DC07. Available from
http://jcdr.net/back_issues.asp?issn=0973-709x&year=2017&month=April&volume=11&issue=4&page=DC04-DC07&id=9720

DOI and Others

DOI: 10.7860/JCDR/2017/23939.9720


Date of Submission: Sep 04, 2016
Date of Peer Review: Sep 28, 2016
Date of Acceptance: Feb 20, 2017
Date of Publishing: Apr 01, 2017


Financial OR OTHER COMPETING INTERESTS: None.

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