Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Original article / research
Year : 2017 | Month : April | Volume : 11 | Issue : 4 | Page : ZC136 - ZC141

Assessment of Enamel Remineralisation After Treatment with Four Different Remineralising Agents: A Scanning Electron Microscopy (SEM) Study

Renita Soares, Ida de Noronha de Ataide, Marina Fernandes, Rajan Lambor

1. Senior Resident, Deprtment of Conservative Dentistry and Endodontics, Goa Dental College and Hospital, Goa, India. 2. Professor and Head of Deprtment, Deprtment of Conservative Dentistry and Endodontics, Goa Dental College and Hospital, Goa, India. 3. Assistant Professor, Deprtment of Conservative Dentistry and Endodontics, Goa Dental College and Hospital, Goa, India. 4. Assistant Professor, Deprtment of Conservative Dentistry and Endodontics, Goa Dental College and Hospital, Goa, India.

Correspondence Address :
Dr. Renita Soares,
Soares Mansion, H. No 202, Telaulim, Navelim, Salcete-Goa, Margao, Goa, India.
E-mail: renita.soares2289@gmail.com

Abstract

Introduction: Decades of research has helped to increase our knowledge of dental caries and reduce its prevalence. However, according to World Oral Health report, dental caries still remains a major dental disease. Fluoride therapy has been utilised in a big way to halt caries progression, but has been met with limitations. This has paved the way for the development of newer preventive agents that can function as an adjunct to fluoride or independent of it.

Aim: The purpose of the present study was to evaluate the ability of Casein Phosphopeptide-Amorphous Calcium Phosphate Fluoride (CPP ACPF), Bioactive Glass (BAG), fluoride enhanced Hydroxyapatite (HA) gel and self-assembling peptide P11-4 to remineralise artificial carious lesions in enamel in vitro using a 30 day pH cycling model through surface microhardness analysis and SEM.

Materials and Methods: Sixty enamel samples were divided into five groups of 12 samples each. The control Group A consisted of intact enamel samples, Group B: CPP-ACPF (Tooth Mousse Plus), Group C: BAG (SHY- NM), Group D: fluoride enhanced HA gel (ReminPro) and Group E: Self-assembling peptide P11-4 (Curodont Protect). All groups excluding the control group were subjected to demineralisation following which four of these groups were remineralised using the four remineralising agents. The treated groups were subjected to pH cycling over a period of 30 days. This was followed by assessment of surface microhardness and SEM for qualitative evaluation of surface changes. The results were analysed by One-Way Analysis Of Variance (ANOVA). Multiple comparisons between groups were performed by paired t-test and post-hoc Tukey test.

Results: The results of the study revealed that remineralisation of enamel was the highest in samples of Group E (Self assembling peptide P11-4) followed by Group B (CPP-ACPF), Group C (BAG) and Group D (fluoride enhanced HA gel). There was a significant difference (p<0.05) in the remineralising ability between the self assembling peptide P11-4 group and BAG and fluoride enhanced HA gel group. Although no significant difference was observed between the self assembling peptide P11-4 and CPP-ACPF group, the self assembling peptide P11-4 remineralised the enamel lesions more effectively. SEM photomicrographs of the test groups demonstrated either amorphous crystals or particles scattered on the surface or lines of remineralisation along the prismatic borders.

Conclusion: Self assembling peptide P11-4 demonstrated promising results by effectively and significantly remineralising the enamel lesions as compared to other test agents.

Keywords

Dental caries, Fluoride, Microhardness, Remineralisation

DOI and Others

DOI: 10.7860/JCDR/2017/23594.9758

Date of Submission: Sep 16, 2016
Date of Peer Review: Nov 10, 2016
Date of Acceptance: Jan 20, 2017
Date of Publishing: Apr 01, 2017

Financial OR OTHER COMPETING INTERESTS: None.

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