Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Original article / research
Year : 2017 | Month : August | Volume : 11 | Issue : 8 | Page : BC10 - BC15

Citral, A Monoterpene Protect Against High Glucose Induced Oxidative Injury in HepG2 Cell In Vitro-An Experimental Study

Sri Devi Subramaniyan, Ashok kumar Natarajan

1. Research Scholar, Department of Biochemistry and Biotechnology, Annamalai University, Chidambaram, Tamil Nadu, India. 2. Assistant Professor, Department of Biochemistry and Biotechnology, Annamalai University, Chidambaram, Tamil Nadu, India.

Correspondence Address :
Dr. Ashok Kumar Natarajan,
Assistant Professor, Department of Biochemistry and Biotechnology,
Annamalai University, Chidambaram-608002, Tamil Nadu, India.
E-mail: npashok@rediffmail.com

Abstract

Introduction: Diabetes mellitus, a major metabolic disorder associated with hyperglycaemia is one of the leading cause of death in many developed countries. However, use of natural phytochemicals have been proved to have a protective effect against oxidative damage.

Aim: To investigate the effect of citral, a monoterpene on high glucose induced cytotoxicity and oxidative stress in human hepatocellular liver carcinoma (Hep G2) cell line.

Materials and Methods: Cells were treated with 50 mM concentration of glucose for 24 hours incubation following citral (30 µM) was added to confluent HepG2 cells. Cell viability, Reactive Oxygen Species (ROS) generation, DNA damage, lipid peroxidation, antioxidants and Mitogen Activated Protein Kinases (MAPKs) signaling were assessed in citral and/or high glucose induced HepG2 cells.

Results: Cells treated with glucose (50 mM), resulted in increased cytotoxicity, ROS generation, DNA damage, lipid peroxidation and depletion of enzymatic and non enzymatic antioxidants. In contrast, treatment with citral (30 µM) significantly decreased cell cytotoxicity, ROS generation, DNA damage, lipid peroxidation and increased antioxidants enzymes in high glucose induced HepG2 cells. In addition, the present study highlighted that high glucose treated cells showed increased expression of Extracellular Signal Regulated Protein Kinase-1 (ERK-1), c-Jun N-terminal Kinase (JNK) and p38 in HepG2 cells. On the other hand treatment with citral significantly suppressed the expression of ERK-1, JNK and p38 in high glucose induced HepG2 cells.

Conclusion: Citral protects against high glucose induced oxidative stress through inhibiting ROS activated MAPK signaling pathway in HepG2 cells.

Keywords

Antioxidants, Hyperglycaemia, Oxidative stress

How to cite this article :

Sri Devi Subramaniyan, Ashok kumar Natarajan. CITRAL, A MONOTERPENE PROTECT AGAINST HIGH GLUCOSE INDUCED OXIDATIVE INJURY IN HEPG2 CELL IN VITRO-AN EXPERIMENTAL STUDY. Journal of Clinical and Diagnostic Research [serial online] 2017 August [cited: 2017 Oct 19 ]; 11:BC10-BC15. Available from
http://jcdr.net/back_issues.asp?issn=0973-709x&year=2017&month=August&volume=11&issue=8&page=BC10-BC15&id=10377

DOI and Others

DOI: 10.7860/JCDR/2017/28470.10377

Date of Submission: Mar 20, 2017
Date of Peer Review: May 09, 2017
Date of Acceptance: Jun 08, 2017
Date of Publishing: Aug 01, 2017

FINANCIAL OR OTHER COMPETING INTERESTS: None.

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