Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Original article / research
Year : 2017 | Month : August | Volume : 11 | Issue : 8 | Page : DC09 - DC11

Isolation and Speciation of Candida in Type II Diabetic Patients using CHROM Agar: A Microbial Study

Usha Sharma, Krupa Patel, Vandana Shah, Surabhi Sinha, Vishnu Pratap Singh Rathore

1. Senior Lecturer, Department of Oral Pathology, K.M. Shah Dental College and Hospital, Sumandeep Vidyapeeth, Vadodara, Gujarat, India. 2. Intern, Department of Oral Pathology, K.M. Shah Dental College and Hospital, Sumandeep Vidyapeeth, Vadodara, Gujarat, India. 3. Professor, Department of Oral Pathology, K.M. Shah Dental College and Hospital, Sumandeep Vidyapeeth, Vadodara, Gujarat, India. 4. Postgraduate Student, Department of Oral Pathology, K.M. Shah Dental College and Hospital, Sumandeep Vidyapeeth, Vadodara, Gujarat, India. 5. Senior Lecturer, Department of Conservative Dentistry and Endodontics, K.M. Shah Dental College and Hospital, Sumandeep Vidyapeeth, Vadodara, Gujarat, India.

Correspondence Address :
Dr. Usha Sharma,
Senior Lecturer, Department of Oral Pathology,
K.M. Shah Dental College and Hospital,
Sumandeep Vidyapeeth, Vadodara-390019, Gujarat, India.
E-mail: ushasharma7@gmail.com

Abstract

Introduction: Newer Candida species are now becoming increasingly predominant commensal in the oral cavity.

Aim:Aim of the study was to identify and compare different Candida species in the oral cavity of Type II diabetic individuals.

Materials and Methods: The present microbial study was carried out for the duration of three months. Sixty participants were included in the study and divided into two groups of 30 individual each. Group I consisted of patients with Type II diabetes while Group II consisted of healthy individuals without diabetes or any other systemic disease. A total of 3 ml of unstimulated whole saliva was collected from them and centrifuged at 5000 rpm for fives minutes. This pellet was plated onto CHROM agar medium plates and incubated at 37°C for at least 3-4 days. CHROM agar plates were visualized daily at 24 hours, 72 hours and followed up to seven days to check for growth. Candida speciation was done by counting the different coloured creamy colonies. Comparison of Candida spp. between two groups was done by applying the Student’s t-test. A p-value<0.05 was considered as statistically significant.

Results: All the species of Candida, namely, Candida albicans, Candida glabrata, Candida dubliniensis, Candida krusei, Candida parapsilosis except for Candida tropicalis showed a significantly higher (p<0.001) occurrence in the diabetic group compared to the healthy group. The highest identified species is C. parapsilosis, second being C. albicans in both the groups.

Conclusion: C. parapsilosis is now considered as one of the significant causes of Candida infection in the oral cavity. This increased virulence will affect the global burden of Candidiasis as few treatment options are available for this new pathogen.

Keywords

Agar medium, Candida parapsilosis, Oral cavity, Prevalence

How to cite this article :

Usha Sharma, Krupa Patel, Vandana Shah, Surabhi Sinha, Vishnu Pratap Singh Rathore. ISOLATION AND SPECIATION OF CANDIDA IN TYPE II DIABETIC PATIENTS USING CHROM AGAR: A MICROBIAL STUDY. Journal of Clinical and Diagnostic Research [serial online] 2017 August [cited: 2017 Oct 24 ]; 11:DC09-DC11. Available from
http://jcdr.net/back_issues.asp?issn=0973-709x&year=2017&month=August&volume=11&issue=8&page=DC09-DC11&id=10394

DOI and Others

DOI: 10.7860/JCDR/2017/24864.10394

Date of Submission: Oct 18, 2016
Date of Peer Review: Jan 20, 2017
Date of Acceptance: May 20, 2017
Date of Publishing: Aug 01, 2017

FINANCIAL OR OTHER COMPETING INTERESTS: None.

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