Gene Scanning for Microdeletions in the Azoospermia Factor Region of Y-Chromosome in Infertile Men of Gujarat, IndiaCorrespondence Address :
Dr. Jenabhai B Chauhan,
Associate Professor and Head, Department of Genetics, Ashok and Rita Patel Institute of Integrated Study and Research
in Biotechnology and Allied Sciences (ARIBAS), New Vallabh Vidyanagar-388121, Dist-Anand, Gujarat, India.
Introduction: Azoospermia Factor (AZF) microdeletions in Yq chromosome is one of the most frequent genetic cause associated with failure of spermatogenesis in males with infertility.
Aim: To figure out the Yq chromosome microdeletions frequency in infertile men from Gujarat region of India.
Materials and Methods: In this study, 141 infertile men with azoospermia (n=41) and oligozoospermia (n=100) were examined along with 159 normozoospermic men. Eleven different markers spanning the azoospermia factor region of human Yq chromosome, amplified by sequence-tagged site Polymerase Chain Reaction (PCR) to detect the microdeletions. Sperm morphological analysis was done using papanicolau staining method.
Results: Thirty four infertile men out of 141 presented Yq chromosome microdeletions. The frequency of AZF microdeletions was 31.71% in azoospermia and 21% in oligozoospermia patients. Only two oligozoospermia patients showed morphological defects.
Conclusion: Due to the presence of high frequency of Yq chromosome microdeletions in Gujarati infertile men, it is imperative to implement the AZF microdeletion screening in such patients as it results in male spermatogenesis dysfunctioning.
Male infertility, Sequence-tagged site polymerase chain reaction, Yq chromosome
Mili Nailwal, Jenabhai B Chauhan. GENE SCANNING FOR MICRODELETIONS IN THE AZOOSPERMIA FACTOR REGION OF Y-CHROMOSOME IN INFERTILE MEN OF GUJARAT, INDIA. Journal of Clinical and Diagnostic Research [serial online] 2017 August [cited: 2017 Oct 24 ]; 11:GC01-GC06. Available from
Date of Submission: Jan 13, 2017
Date of Peer Review: Mar 04, 2017
Date of Acceptance: Jun 17, 2017
Date of Publishing: Aug 01, 2017
FINANCIAL OR OTHER COMPETING INTERESTS: None.
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