Comparative Study on the Estimation of Estrous Cycle in Mice by Visual and Vaginal Lavage MethodCorrespondence Address :
Dr. Senthil Kumar Sampath Kumar,
Professor, Department of Anatomy, Sri Ramachandra Medical College and Research Institute,
Chennai - 600116, Tamil Nadu, India.
Introduction: Evaluation of estrous cycle in laboratory animals can be a useful measure of the integrity of hypothalamic-pituitary-ovarian reproductive axis. Assessment of vaginal cytology is crucial to assess the milieu and compare the endocrine status of animals among the experimental groups.
Aim: The present study was attempted to compare the estimation of estrous cycle by visual method and non invasive vaginal lavage method.
Materials and Methods: Sixty healthy female swiss albino mice were used for the present study. The appearance of the vagina with respect to the opening of vagina, vaginal swellings were observed. Non-invasive method was used in vaginal lavage method in which nucleated epithelial cells, cornified squamous epithelial cells and leucocytes present in vaginal smears were used to identify the estrous stages.
Results: The estimation of estrous cycle by visual method coincides with the vaginal lavage method. In Vaginal lavage method, the accurate proportion of cells and the transition phases can be evaluated.
Conclusion: The non-invasive method reduces the risk of pseudo -pregnancy and mechanical trauma. Though, visual method is quick and reliable, for accurate estimation of the stage of the estrous, non-invasive vaginal lavage method is ideal.
Laboratory animals, Reproductive cycle, Vaginal cell morphology, Vaginal smears
Gnanagurudasan Ekambaram, Senthil Kumar Sampath Kumar, Leena Dennis Joseph. COMPARATIVE STUDY ON THE ESTIMATION OF ESTROUS CYCLE IN MICE BY VISUAL AND VAGINAL LAVAGE METHOD. Journal of Clinical and Diagnostic Research [serial online] 2017 January [cited: 2017 Jan 18 ]; 11:AC05-AC07. Available from
Date of Submission: Sep 06, 2016
Date of Peer Review: Oct 11, 2016
Date of Acceptance: Nov 01, 2016
Date of Publishing: Jan 01, 2017
Financial OR OTHER COMPETING INTERESTS: None.
- PubMed Central® (PMC)New
- Academic Search Complete Database
- Chemical Abstracts Service
- Directory of Open Access Journals (DOAJ)
- Embase & EMbiology
- Google Scholar
- HINARI Access to Research in Health Programme
- Indian Science Abstracts (ISA)
- Journal seek Database
- Open J-Gate
- Popline (reproductive health literature)