Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Original article / research
Year : 2017 | Month : July | Volume : 11 | Issue : 7 | Page : DC19 - DC23

Distribution of Class D Carbapenemase and Extended-Spectrum b-Lactamase Genes among Acinetobacter Baumannii Isolated from Burn Wound and Ventilator Associated Pneumonia Infections

Maryam Mohammadi, Setareh Soroush, Somayeh Delfani, Iraj Pakzad, Abolfazl Abbaszadeh, Mahmoud Bahmani, Lidija Bogdanovic, Morovat Taherikalani

1. Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran. 2. Razi Herbal Medicines Research Center, Department of Microbiology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran. 3. Razi Herbal Medicines Research Center, Department of Microbiology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran. 4. Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran. 5. Department of Surgery, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran. 6. Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam Iran. 7. Department of Public Health, University of Naples Federico II, Naples, Italy. 8. Razi Herbal Medicines Research Center, Department of Microbiology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.

Correspondence Address :
Prof. Morovat Taherikalani,
Razi Herbal Medicines Research Center, Department of Microbiology, School of Medicine, Khorramabad, Iran.
E-mail: taherikalani@gmail.com

Abstract

Introduction: Resistance to Acinetobacter baumannii is dramatically on the rise in Iran. Therefore, it is important to study resistance pattern among Acinetobacter isolates which is a common cause of nosocomial infections.

Aim: To investigate antibiotic resistance patterns and the role of resistant genes and biofilm formation in the induction of resistance among Acinetobacter baumannii isolated from burn wound and ventilator associated pneumonia infections.

Materials and Methods: Total 103 isolates such as 33 burn samples from Rasool Akram Hospital and 70 isolates from ventilated patients in Shahid Motahhari Hospital were identified with A. baumannii using biochemical method, and then identified to species level with PCR of gyrB and blaOXA-51 gene. Antibiotic sensitivity pattern for b-lactam and carbapenem antibiotics was assessed using Agar disc diffusion test and E-test. The presence of different carbapenemase and metalo-b-lactamase (blaOXA-51-like, gyrB, blaOXA-23-like, blaOXA-24-like, blaOXA-58, blaVEB, blaPER, blaGIM, blaSIM, blaIMP, blaVIM), extended-spectrum ß-lactamases (blaTEM, blaSHV) and two insertion sequences genes (ISaba1, IS1113) was assessed. Biofilm formation of all isolates was then assessed. Chi-square analysis or Fisher’s-exact tests were used for statistical analysis. A p-value <0.05 was considered statistically significant.

Results: Colistin was the most effective antimicrobial agents, although 10.7% (11/103) of the isolates were resistant. The high rate of resistance to meropenem (93.2%) and imipenem (90.3%) was determined. Also, with exception of ampicillin-sulbactam, surprisingly the resistant rate was 28.2%, the resistance to b-lactam antibiotic was dramatically increased. Co-existence of two and three blaOXA genes was also determined. The blaOXA-58 was detected in only one isolate. The blaTEM and blaOXA-23 was the most prevalent Extended-Spectrum ß-Lactamases (ESBL) gene. All isolates were biofilm producers.

Conclusion: Antibiotic resistance is increasing among A. baumannii isolates which is due to excessive use of antibiotics and also acquired resistant genes and biofilm production. Resistance to nearly all antimicrobial agents especially colistin as end choice for treatment of multiple drug resistance A. baumannii is a big concern.

Keywords

b-lactams, Insertion elements, Nosocomial infections

How to cite this article :

Maryam Mohammadi, Setareh Soroush, Somayeh Delfani, Iraj Pakzad, Abolfazl Abbaszadeh, Mahmoud Bahmani, et al.. DISTRIBUTION OF CLASS D CARBAPENEMASE AND EXTENDED-SPECTRUM B-LACTAMASE GENES AMONG ACINETOBACTER BAUMANNII ISOLATED FROM BURN WOUND AND VENTILATOR ASSOCIATED PNEUMONIA INFECTIONS. Journal of Clinical and Diagnostic Research [serial online] 2017 July [cited: 2017 Sep 21 ]; 11:DC19-DC23. Available from
http://jcdr.net/back_issues.asp?issn=0973-709x&year=2017&month=July&volume=11&issue=7&page=DC19-DC23&id=10218

DOI and Others

DOI: 10.7860/JCDR/2017/25534.10218


Date of Submission: Nov 19, 2016
Date of Peer Review: Jan 06, 2017
Date of Acceptance: Apr 10, 2017
Date of Publishing: Jul 01, 2017


Financial OR OTHER COMPETING INTERESTS: None.

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