Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Original article / research
Year : 2017 | Month : July | Volume : 11 | Issue : 7 | Page : EC12 - EC16

Immunohistochemical Study of MUC1 and MUC5AC Expression in Gall Bladder Lesions

Amit Bhoge, Siddhi Gaurish Sinai Khandepaparkar, Avinash R Joshi, Bageshri Gogate, Maithili Mandar Kulkarni, Pallavi Bhayekar

1. Postgraduate Student, Department of Pathology, Smt. Kashibai Navale Medical College and General Hospital, Pune, Maharashtra, India. 2. Associate Professor, Department of Pathology, Smt. Kashibai Navale Medical College and General Hospital, Pune, Maharashtra, India. 3. Professor, Department of Pathology, Smt. Kashibai Navale Medical College and General Hospital, Pune, Maharashtra, India. 4. Professor, Department of Pathology, Smt. Kashibai Navale Medical College and General Hospital, Pune, Maharashtra, India. 5. Associate Professor, Department of Pathology, Smt. Kashibai Navale Medical College and General Hospital, Pune, Maharashtra, India. 6. Assistant Professor, Department of Pathology, Smt. Kashibai Navale Medical College and General Hospital, Pune, Maharashtra, India.

Correspondence Address :
Dr. Siddhi Gaurish Sinai Khandeparkar,
E-517, The Island, Wakad, Pune-411057, Maharashtra, India.
E-mail: siddhigsk@yahoo.co.in

Abstract

Introduction: Immunohistochemical (IHC) markers of mucin family are associated with various Gallbladder Lesions (GBLs).

Aim: To study the distribution of GBL with respect to age and sex as well as to analyse the IHC profile of MUC1 and MUC5AC in GBLs and attempt correlation with clinical and histopathological findings.

Materials and Methods: The present study was conducted over a period of six years. A technique of manual tissue array was employed for cases subjected to IHC using MUC1 and MUC5AC. Results were statistically analysed using software program “The Primer of Biostatistics 5.0”.

Results: A total of 629 GBL were encountered. Out of 605 of non-neoplastic lesions, 32 (5.29%) expressed MUC1 while 515 (85.12%) cases expressed MUC5AC. Out of 24 cases of neoplastic GBL, 20 cases (83.33%) showed positivity for MUC1 and 9 cases (37.5%) were positive for MUC5AC. The rate of MUC1 expression was significantly higher in Gall Bladder Cancer (GBC) {18GB carcinoma (ca) +3 Carcinoma In Situ (CIS)} (85.71%) than chronic cholecystitis (4.71%). The positive rate of MUC5AC expression was significantly lower in GBC (28.57%) than chronic cholecystitis (87.19%). The percentage of cases showing MUC1 expression increased as the severity of disease progressed from hyperplasia to CIS. The percentage of cases showing MUC5AC expression decreased as the severity of disease progressed from hyperplasia to CIS.

Conclusion: In this study, 96.18% cases were non neoplastic GBL of which chronic cholecystitis (87.77%) was predominant. 3.81% of the GBL constituted for neoplastic lesions of which 75% were GBC. MUC1 showed higher rates of expression in neoplastic GBL. MUC5AC showed higher rates of expression in non neoplastic GBL. Expression of MUC1 and MUC5AC might be closely related to pathogenesis of neoplastic and non neoplastic GBL.

Keywords

Dysplasia, Gall bladder carcinoma, Metaplasia, Mucin markers

How to cite this article :

Amit Bhoge, Siddhi Gaurish Sinai Khandepaparkar, Avinash R Joshi, Bageshri Gogate, Maithili Mandar Kulkarni, Pallavi Bhayekar. IMMUNOHISTOCHEMICAL STUDY OF MUC1 AND MUC5AC EXPRESSION IN GALL BLADDER LESIONS. Journal of Clinical and Diagnostic Research [serial online] 2017 July [cited: 2017 Jul 21 ]; 11:EC12-EC16. Available from
http://jcdr.net/back_issues.asp?issn=0973-709x&year=2017&month=July&volume=11&issue=7&page=EC12-EC16&id=10230

DOI and Others

DOI: 10.7860/JCDR/2017/26537.10230


Date of Submission: Jan 04, 2017
Date of Peer Review: Feb 14, 2017
Date of Acceptance: Apr 17, 2017
Date of Publishing: Jul 01, 2017


Financial OR OTHER COMPETING INTERESTS: None.

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