Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Original article / research
Year : 2017 | Month : July | Volume : 11 | Issue : 7 | Page : FC06 - FC10

Comparison of Pioglitazone and Metformin Efficacy against Glucocorticoid Induced Atherosclerosis and Hepatic Steatosis in Insulin Resistant Rats

I M Nagendra Nayak, Koyagura Narendar, Patil Ashok M, M G Jamadar, V Hemanth Kumar

1. Professor and Head, Department of Pharmacology, Mount Zion Medical College, Adoor, Kerala, India. 2. Lecturer, Department of Pharmacology, Al-Ameen Medical College, Vijayapura, karnataka, India. 3. Professor and Head, Department of Pathology, Al-Ameen Medical College, Vijayapura, karnataka, India. 4. Professor and Head, Department of Pharmacology, Al-Ameen Medical College, Vijayapura, Karnataka, India. 5. Lecturer, Department of Pharmacology, Al-Ameen Medical College, Vijayapura, karnataka, India.

Correspondence Address :
Dr. Koyagura Narendar,
Lecturer, Department of Pharmacology, Al Ameen Medical College, Vijayapura-586108, Karnataka, India.
E-mail : narendarkoyagura@gmail.com

Abstract

Introduction: Insulin Resistance is a major cause of Atherosclerosis (AS) and Non Alcoholic Fatty Liver Disease (NAFLD). These lipid alterations in blood vessels and liver may progress to cardiovascular abnormalities and cirrhosis respectively. Drugs like pioglitazone (PIO) and metformin (MET) are effective insulin sensitizers used in T2DM. But their efficacy and tolerability needs to be compared in IR associated abnormalities.

Aim: To compare the efficacy of PIO and MET in glucocorticoid induced AS, Hepatic Steatosis (HS) and IR in albino rats.

Materials and Methods: Male Wistar albino rats were randomized into four groups (n=6). Group 1 (Normal control) rats consumed 2% gum acacia orally for 12 days. Group 2 {dexamethasone (DEX) control} rats were administered 2% gum acacia orally for 12 days and DEX (8 mg/kg) intraperitoneally (i.p.) from 7th to 12th day during the study period. Group 3 and 4 (PIO and MET control) rats received oral administration of PIO (45 mg/kg) and MET (1000 mg/kg) for 12 days respectively. Both groups were treated with DEX (8 mg/kg/i.p.) from 7th to 12th day during the study period. On last day, fasting blood was collected and rats were sacrificed by cervical dislocation; aorta and liver tissues were isolated for the histopathological examination. Body weight, liver weight and liver volume were measured. Blood samples were processed for biochemical parameters. The data were analysed by One-way Analysis of variance (ANOVA) followed by Scheffe’s multiple comparison post-hoc test. The statistical significance was assumed at p<0.05.

Results: Our results established the possible role of DEX in the development of AS and HS. Histopathological examination of Group 2 rats treated with DEX showed a marked lipid accumulation in the aorta and liver. Administration of MET and PIO resulted in partial to complete restoration of DEX induced fatty changes in aorta and liver. Both drugs significantly (p<0.05) prevented the elevation of insulin, lipid, glucose levels, liver weight and liver volume in DEX treated rats. They had significantly (p<0.05) improved body weight and insulin sensitivity. However, PIO was highly significant (p<0.05) compared to MET in reducing DEX induced IR complications.

Conclusion: These findings suggest that PIO was more effective insulin sensitizer compared to MET in reducing AS, HS and IR induced by glucocorticoids.

Keywords

Dexamethasone, Dyslipidemia, Fatty changes, Insulin sensitizers

How to cite this article :

I M Nagendra Nayak, Koyagura Narendar, Patil Ashok M, M G Jamadar, V Hemanth Kumar. COMPARISON OF PIOGLITAZONE AND METFORMIN EFFICACY AGAINST GLUCOCORTICOID INDUCED ATHEROSCLEROSIS AND HEPATIC STEATOSIS IN INSULIN RESISTANT RATS. Journal of Clinical and Diagnostic Research [serial online] 2017 July [cited: 2017 Sep 20 ]; 11:FC06-FC10. Available from
http://jcdr.net/back_issues.asp?issn=0973-709x&year=2017&month=July&volume=11&issue=7&page=FC06-FC10&id=10193

DOI and Others

DOI: 10.7860/JCDR/2017/28418.10193


Date of Submission: Mar 20, 2017
Date of Peer Review: Apr 11, 2017
Date of Acceptance: May 16, 2017
Date of Publishing: Jul 01, 2017


Financial OR OTHER COMPETING INTERESTS: None.

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