Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Experimental Research
Year : 2017 | Month : July | Volume : 11 | Issue : 7 | Page : TF01 - TF04

Evaluation of the Potency of Kinetin on Radiation Induced Behavioral Changes in Swiss Albino Mice

Vishakh Radhakrishna, Suchetha Kumari Nanilu, Ganesh Sanjeev, Jayarama Shetty, Yogish Tenkanidiyoor Somyaji, Shailaja Shivarama Moodithaya

1. Research Scholar, Department of Biochemistry, Central Research Labratory, K.S. Hegde Medical Academy, Nitte University, Mangalore, Karnataka, India. 2. Professor, Department of Biochemistry, K.S. Hegde Medical Academy, Nitte University, Mangalore, Karnataka, India. 3. Professor, Department of Physics and Head and In charge of Microtron Center, Mangalagangotri, Mangalore University, Mangalore, Karnataka, India. 4. Professor, Department of Radiation Oncology, K. S. Hegde Charitable Hospital, Nitte University, Mangalore, Karnataka, India. 5. Research Scholar, Department of Department of Biochemistry, Central Research Labratory, K.S. Hegde Medical Academy, Nitte University, Mangalore, Karnataka, India. 6. Additional Professor, Department of Physiology, K.S. Hegde Medical Academy, Nitte University, Mangalore, Karnataka, India.

Correspondence Address :
Dr. Suchetha Kumari Nanilu,
Professor, Department of Biochemistry, K. S. Hegde Medical Academy,
Deralakatte - 575 018, Mangalore, Karnataka, India.
E-mail: suchetha.shetty@rediffmail.com

Abstract

Introduction: According to the various independent studies conducted, it is well evident fact that radiation induces oxidative stress in the living system. It is also proved that this oxidative stress will lead to the various behavioral changes such as anxiety and memory impairment. Kinetin is one of the important plant cytokine with anti-aging properties. However, very few studies were conducted to check its potential in ameliorating the behavioral changes induced by the ionizing radiation.

Aim: This study was aimed to check the potential of kinetin in ameliorating the radiation induced behavioral changes in albino mice.

Materials and Methods: In this study, survival analysis was performed using three different dose of kinetin intervention along with, one radiation control group and one normal control group (n=50). Based on the cumulative survival rate, single effective dose of kinetin was selected and used to evaluate the behavioral changes induced by radiation. The open field apparatus was used to evaluate the anxiety level (n=18, six in each group). Eight armed radial maze was used to evaluate the memory and learning ability in mice model.

Results: Survival study results suggest 100 mg/kg body weight of kinetin showed highest cumulative survival rate. Therefore, this dose was selected as an effective drug dose for further study. Analysis also showed 6 Gy whole body electron beam radiation had significantly increased anxiety level, increased duration to complete the task as well as mistakes done during the task. Further, kinetin intervention had significantly ameliorated the same.

Conclusion: A 100 mg/kg body weight of kinetin intervention helps in reducing the anxiety and improves the learning ability in mice exposed to electron beam radiation.

Keywords

Anxiety, Oxidative stress, Radial maze

How to cite this article :

Vishakh Radhakrishna, Suchetha Kumari Nanilu, Ganesh Sanjeev, Jayarama Shetty, Yogish Tenkanidiyoor Somyaji, Shailaja Shivarama Moodithaya . EVALUATION OF THE POTENCY OF KINETIN ON RADIATION INDUCED BEHAVIORAL CHANGES IN SWISS ALBINO MICE. Journal of Clinical and Diagnostic Research [serial online] 2017 July [cited: 2017 Jul 20 ]; 11:TF01-TF04. Available from
http://jcdr.net/back_issues.asp?issn=0973-709x&year=2017&month=July&volume=11&issue=7&page=TF01-TF04&id=10226

DOI and Others

DOI: 10.7860/JCDR/2017/25171.10226


Date of Submission: Nov 03, 2016
Date of Peer Review: Dec 08, 2016
Date of Acceptance: May 27, 2017
Date of Publishing: Jul 01, 2017

FINANCIAL OR OTHER COMPETING INTERESTS: None

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