Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Original article / research
Year : 2017 | Month : July | Volume : 11 | Issue : 7 | Page : ZC25 - ZC28

Periodontal Initial Radiological Findings of Genetically Predisposed Finnish Adolescents.

Anna Maria Heikkinen, Elmira Pakbaznejad Esmaeili, Leena Kovanen, Hellevi Ruokonen, Kaisa Kettunen, Jari Haukka, Taina Tervahartiala, Timo Sorsa

1. Department of Oral and Maxillofacial Diseases, Head and Neck Center, University of Helsinki, Clinicum, Helsinki, Uusimaa, Finland. 2. Department of Oral and Maxillofacial Diseases, Head and Neck Center, University of Helsinki, Clinicum, Helsinki, Uusimaa, Finland. 3. Department of Oral and Maxillofacial Diseases, Head and Neck Center, University of Helsinki, Clinicum, Helsinki, Uusimaa, Finland. 4. Department of Oral and Maxillofacial Diseases, Head and Neck Center, University of Helsinki, Clinicum, Helsinki, Uusimaa, Finland. 5. FIMM, Institute for Molecular Medicine Finland, Helsinki, Uusimaa, Finland. 6. Docent, Department of Public Health, Clinicum, University of Helsinki, Uusimaa, Finland. 7. Docent, Department of Oral and Maxillofacial Diseases, Head and Neck Center, University of Helsinki, Clinicum, Helsinki, Uusimaa, Finland. 8. Professor, Department of Oral and Maxillofacial Diseases, Head and Neck Center, University of Helsinki, Clinicum, Helsinki, Uusimaa, Finland.

Correspondence Address :
Dr. Anna Maria Heikkinen,
Department of Oral and Maxillofacial Diseases, Head and Neck Center,
University of Helsinki and Helsinki, Clinicum, Helsinki, Uusimaa, Finland,
Haartmaninkatu 8, FI-00290 Helsinki, Finland.
E-mail: anna.m.heikkinen@helsinki.fi

Abstract

Introduction: Periodontitis is a multifactorial infectious disease of the supporting tissues of teeth in which bacterial, genetic and lifestyle factors such as smoking have an important role.

Aim: The aim was to examine if Bleeding On Probing (BOP = 20%) and = 4 mm deep pockets correlated with any suspicion of initial radiological findings of periodontitis and bone loss. We also investigated whether any pro-inflammatory-related candidate Single Nucleotide Polymorphisms (SNPs) were associated with any suspicion of radiological findings.

Materials and Methods: Altogether 47 generally healthy adolescent patients of one birth cohort had given their approval for their saliva samples to be used for DNA analysis. One participant was excluded after discrepant gender check. An oral radiologist analysed right and left bitewing radiographs of 47 patients. Clinical parameters such as BOP = 20%, = 4 mm pockets, Visible Plaque Index of all teeth (VPI%), as well as smoking habits were recorded. DNA was extracted and 71 SNPs from candidate genes for initial periodontitis were genotyped. The association between = 4 mm pockets and BOP = 20% with radiological findings and selected SNPs was modelled using logistic regression.

Results: Variants in Toll-Like Receptors 4 (TLR4) gene (rs498670) (OR=5.8, {CI95% 1.6–20.7}, p=0.02, FDR q-value=0.13) and TNFSF11 gene (rs2277438, OR=0.3 {CI95% 0.1–0.9}, p=0.002, FDR q-value=0.56) were associated with any suspicious radiological findings; however the significance vanished after False Discovery Rate analysis (FDR). The association between BOP = 20% and any radiographic signs of periodontitis was found to be statistically significant, OR=1.6, CI 95% 1.0-2.4, p=0.04.

Conclusion: Only TLR4 (rs498670) and TNFSF11 (rs2277438) genes were found to have a positive correlation with radiological findings suggestive of initial periodontitis after adjustment for smoking and visible plaque.

Keywords

Genes, Periodontitis, X-rays

How to cite this article :

Anna Maria Heikkinen, Elmira Pakbaznejad Esmaeili, Leena Kovanen, Hellevi Ruokonen, Kaisa Kettunen, Jari Haukka, et al.. PERIODONTAL INITIAL RADIOLOGICAL FINDINGS OF GENETICALLY PREDISPOSED FINNISH ADOLESCENTS.. Journal of Clinical and Diagnostic Research [serial online] 2017 July [cited: 2017 Jul 21 ]; 11:ZC25-ZC28. Available from
http://jcdr.net/back_issues.asp?issn=0973-709x&year=2017&month=July&volume=11&issue=7&page=ZC25-ZC28&id=10168

DOI and Others

DOI: 10.7860/JCDR/2017/25850.10168



Date of Submission: Dec 09, 2016
Date of Peer Review: Feb 13, 2017
Date of Acceptance: Apr 11, 2017
Date of Publishing: Jul 01, 2017


Financial OR OTHER COMPETING INTERESTS: None.

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