Evaluation of CYP1B1 Expression, Oxidative Stress and Phase 2 Detoxification Enzyme Status in Oral Squamous Cell Carcinoma PatientsCorrespondence Address :
Dr. Rahul Saxena,
Assistant Professor, Department of Biochemistry, School of Allied Health Sciences, Sharda University,
Plot No. 32-34, Knowledge Park III, Greater Noida-201306, Uttar Pradesh, India.
Introduction: Oral Squamous Cell Carcinoma (OSCC) affects global health with increasing incidence and mortality rate. It may involve exposure to carcinogens from tobacco smoking or chewing. Alteration in expression of gene encoding the enzymes concerned with carcinogen biotransformation along with oxidative stress may increase or decrease the risk of cancer.
Aim: To evaluate the expression of CYP1B1 gene in OSCC patients along with its relation with oxidative stress and phase 2 detoxification enzyme status.
Materials and Methods: In the present study, CYP1B1 genotypic analysis was carried out along with estimation of serum Total Antioxidant Activity (TAA), erythrocyte Malondialdehyde (MDA) levels and serum Glutathione-S-Transferase (GST) activity in 20 OSCC patients and statistically compared with that of age matched 20 healthy subjects, served as control by using student’s t-test.
Results: It was observed that 85% of histopathologically diagnosed OSCC patients had CYP1B1 expression with significantly elevated levels of MDA (p<0.001). In addition, plasma total antioxidant status and serum GST levels were decreased significantly (p<0.05) in OSCC patients as compared to the healthy controls to overcome the burden of oxidative stress.
Conclusion: On the basis of the present study, we conclude that the expression of CYP1B1 is an important determinant of carcinogenesis and significantly associated with oxidative stress characterized by decreased serum GST and total antioxidant levels in OSCC patients.
Cytochrome P450, Free radicals, Glutathione S transferase, Oral cancer, Total antioxidant status
Shailza Verma, Rahul saxena, Merajul Haque Siddiqui, Karunathy Santha, Subramaniam Sethupathy. EVALUATION OF CYP1B1 EXPRESSION,
OXIDATIVE STRESS AND PHASE 2
DETOXIFICATION ENZYME STATUS IN ORAL
SQUAMOUS CELL CARCINOMA PATIENTS. Journal of Clinical and Diagnostic Research [serial online] 2017 March [cited: 2017 Apr 28 ]; 11:BC01-BC05. Available from
Date of Submission: Jun 20, 2016
Date of Peer Review: Jul 21, 2016
Date of Acceptance: Oct 10, 2016
Date of Publishing: Mar 01, 2017
Financial OR OTHER COMPETING INTERESTS: None.
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