Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Original article / research
Year : 2017 | Month : March | Volume : 11 | Issue : 3 | Page : DC14 - DC17

Rapid Identification of Vancomycin Resistant Enterococcus Faecalis Clinical Isolates using a Sugar Fermentation Method

Javad Raeisi, Mahnaz Saifi, Mohammad Reza Pourshafie, Mehri Habibi, Hamid Reza Mohajerani, Neda Akbari, Mohammad Reza Asadi Karam

1. Postgraduate Student, Department of Microbiology, Pasteur Institute of Iran, Pasteur Ave, Tehran, Iran. 2. Associate Professor, Department of Mycobacteriology, Pasteur Institute of Iran, Pasteur Ave, Tehran, Iran. 3. Professor, Department of Microbiology, Pasteur Institute of Iran, Pasteur Ave, Tehran, Iran. 4. Assistant Professor, Department of Molecular Biology, Pasteur Institute of Iran, Pasteur Ave, Tehran, Iran. 5. Assistant Professor, Department of Microbiology, Islamic Azad University, Arak branch, Arak, Iran. 6. Assistant Professor, Department of Microbiology, Islamic Azad University, Arak branch, Arak, Iran. 7. Assistant Professor, Department of Molecular Biology, Pasteur Institute of Iran, Pasteur Ave, Tehran, Iran.

Correspondence Address :
Dr. Mohammad Reza Asadi Karam,
Assistant Professor, Department of Molecular Biology, Pasteur Institute of Iran, Pasteur Ave, Tehran -13164, Iran.
E-mail: m_asadi12@yahoo.com

Abstract

Introduction: Vancomycin Resistant Enterococci (VRE) can be found all over the world. Thus, rapid detection of the isolates could be of high importance in the treatment or prevention of the associated disease.

Aim: To measure the turanose fermentation in Enterococcus faecalis clinical isolates for rapid differentiation of VRE and Vancomycin-Susceptible E. faecalis (VSE) isolates.

Materials and Methods: Forty E. faecalis samples were isolated from 200 clinical samples in Tehran Medical Center, Iran, from October 2012 to December 2012. These isolates were detected according to the standard microbial and biochemical tests. Detection of VRE isolates was originally performed by disk diffusion using 1 µg vancomycin disk, followed by Polymerase Chain Reaction (PCR) amplification of the vanA gene. Finally, the turanose consumption in 1%, 0.7% and 0.5% dilutions was detected by a phenotypic method.

Results: Among the 40 E. faecalis isolates, 20 vancomycin-susceptible and 20 vancomycin-resistant E. faecalis were isolated according to the disk diffusion and PCR of the vanA gene. There was a considerable difference between VRE and VSE isolates in 0.7% dilution of turanose. However, there was no significant difference between VRE and VSE in 1% and 0.5% dilutions of turanose.

Conclusion: Since detection of VRE isolates is of high importance, especially in nosocomial infections, phenotypic methods may be highly useful for this purpose. In conclusion, our data indicate that VRE isolated from clinical samples could be distinguished from VSE isolates by turanose fermentation at dilution 0.7%.

Keywords

Antibiotic resistance, Phenotypic methods, Turanose consumption

How to cite this article :

Javad Raeisi, Mahnaz Saifi, Mohammad Reza Pourshafie, Mehri Habibi, Hamid Reza Mohajerani, Neda Akbari, et al.. RAPID IDENTIFICATION OF VANCOMYCIN RESISTANT ENTEROCOCCUS FAECALIS CLINICAL ISOLATES USING A SUGAR FERMENTATION METHOD. Journal of Clinical and Diagnostic Research [serial online] 2017 March [cited: 2017 Mar 26 ]; 11:DC14-DC17. Available from
http://jcdr.net/back_issues.asp?issn=0973-709x&year=2017&month=March&volume=11&issue=3&page=DC14-DC17&id=9568

DOI and Others

DOI: 10.7860/JCDR/2017/19017.9568


Date of Submission: Jan 23, 2016
Date of Peer Review: Feb 29, 2016
Date of Acceptance: Jun 29, 2016
Date of Publishing: Mar 01, 2016

Financial OR OTHER COMPETING INTERESTS: None.

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