Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Original article / research
Year : 2017 | Month : November | Volume : 11 | Issue : 11 | Page : ZC05 - ZC08

Comparison of Staining Efficacy between Turmeric and Eosin: A Histological Study

Marin Abraham, Shwetha Nambiar, Vanishri Charagannavar, Dominic Augustine, SV Sowmya, Ashok Babu, Roopa S Rao

1. Postgraduate Student, Department of Oral Pathology, MS Ramaiah University of Applied Sciences, Bengaluru, Karnataka, India. 2. Tutor, Department of Oral Pathology, MS Ramaiah University of Applied Sciences, Bengaluru, Karnataka, India. 3. Assistant professor, Department of Oral Pathology, MS Ramaiah University of Applied Sciences, Bengaluru, Karnataka, India. 4. Assistant professor, Department of Oral Pathology, MS Ramaiah University of Applied Sciences, Bengaluru, Karnataka, India. 5. Assistant professor, Department of Oral Pathology, MS Ramaiah University of Applied Sciences, Bengaluru, Karnataka, India. 6. Professor and Head, Department of Pharmacognosy, Faculty of Pharmacy, Ramaiah University of Applied Sciences, Bengaluru, Karnataka, India. 7. Professorand Head, Department of Oral Pathology, MS Ramaiah University of Applied Sciences, Bengaluru, Karnataka, India.

Correspondence Address :
Dr. Marin Abraham,
Postgraduate Student, Department of Oral Pathology and Microbiology, Faculty of Dental Sciences,
MS Ramaiah University of Applied Sciences, Bengaluru, Karnataka, India.
E-mail: marinabraham09@gmail.com

Abstract

Introduction: Turmeric-Curcuma longa is a natural dye and has been used for various purposes since centuries.The global interest to use biodegradable materials; natural dyes/stains has evoked curiosity to explore its staining efficacy. Limited studies have been performed to determine the efficacy of turmeric as a stain for formalin fixed paraffin embedded sections that are routinely stained with Hematoxylin and Eosin (H&E). This study highlights on the comparison of turmeric extraction methods namely, maceration and soxhlet in relation to its staining efficacy which is first of its kind. Also, the staining of turmeric extracts on pathological tissues has not been tried earlier.

Aim: To compare two different extraction methods for turmeric namely the maceration and soxhlet, to compare staining efficacy of turmeric with and without mordant, to compare efficacy of turmeric stain with synthetic eosin in normal and pathological tissues.

Materials and Methods: Curcuma longa was dried, powdered and extracted by maceration and soxhlet techniques. Tissue sections were stained with and without the mordant potash alum in normal and pathologic tissues. The staining intensity, efficacy of prepared turmeric stain with that of eosin were compared by appreciating the respective tissue structures stained by both the components. The statistical analysis was carried out by Chi-square test.

Results: Statistically significant results were observed in sections stained by maceration technique than that of the soxhlet. The Hematoxylin and Turmeric (H&T) sections in which Mordant was incorporated with Turmeric (TEM) fetched better results. Comparable results were obtained in normal and pathologic tissue sections stained with H&T (TEM) with conventionally employed H&E stain.

Conclusion: Turmeric extract can be used as an adjunct stain to eosin, a synthetic stain.

Keywords

Curcumin, Maceration, Mordant, Soxhlet

How to cite this article :

Marin Abraham, Shwetha Nambiar, Vanishri Charagannavar, Dominic Augustine, SV Sowmya, Ashok Babu, et al.. COMPARISON OF STAINING EFFICACY BETWEEN TURMERIC AND EOSIN: A HISTOLOGICAL STUDY. Journal of Clinical and Diagnostic Research [serial online] 2017 November [cited: 2017 Nov 19 ]; 11:ZC05-ZC08. Available from
http://jcdr.net/back_issues.asp?issn=0973-709x&year=2017&month=November&volume=11&issue=11&page=ZC05-ZC08&id=10809

DOI and Others

DOI: 10.7860/JCDR/2017/26024.10809

Date of Submission: Dec 15, 2016
Date of Peer Review: Feb 17, 2017
Date of Acceptance: Jul 09, 2017
Date of Publishing: Nov 01, 2017

FINANCIAL OR OTHER COMPETING INTERESTS: None.

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