Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Original article / research
Year : 2017 | Month : October | Volume : 11 | Issue : 10 | Page : ZC11 - ZC16

Raman Microspectrometry: An Alternative Method of Age Estimation from Dentin and Cementum

Karuna Kumari, Roopa S Rao, Sachin Chakradhar Sarode, Gargi Sachin Sarode, Shankargouda Patil

1. Postgraduate Student, Department of Oral Pathology and Microbiology, Faculty of Dental Sciences, Ramaiah University of Applied Sciences, Bengaluru, Karnataka, India. 2. Professor and Head, Department of Oral Pathology and Microbiology, Faculty of Dental Sciences, MS Ramaiah University of Applied Sciences, Bengaluru, Karnataka, India. 3. Professor, Department of Oral Pathology and Microbiology, Dr. D. Y. Patil Dental College and Hospital, Dr. D. Y. Patil Vidyapeeth, Pune, Maharashtra, India. 4. Professor, Department of Oral Pathology and Microbiology, Dr. D. Y. Patil Dental College and Hospital, Dr. D. Y. Patil Vidyapeeth, Pune, Maharashtra, India. 5. Associate Professor, Department of Oral and Maxillofacial Surgery, Division of Oral Pathology, College of Dentistry, Jazan University, Jazan, Kingdom of Saudi Arabia.

Correspondence Address :
Dr. Sachin Chakradhar Sarode,
Professor, Department of Oral Pathology and Microbiology, Dr. D. Y. Patil Dental College and Hospital,
Dr. D.Y. Patil Vidyapeeth, Sant-Tukaram Nagar, Pimpri, Pune-411018, Maharashtra, India.
E-mail: drsachinsarode@gmail.com

Abstract

Introduction: Raman spectroscopy is simple, quick, sensitive and non destructive form of tissue examination that provides vital data about the structure, molecular composition and interactions within a sample. The human hard tissues like teeth and bone are able to resist decay for long even after other tissues are lost, thus have valuable forensic importance.

Aim: To ascertain the known age of the teeth by analysing dentin and cementum using Raman microspectrometry and assess the accuracy of age estimation by comparison of dentin with cementum.

Materials and Methods: The sound permanent extracted tooth specimens (40) of age ranging between 12-74 years were collected and sectioned longitudinally and different dentinal and cemental areas were analysed by Raman microspectrometry. The spectra of dentin and cementum were used as predictors of age estimation. For each sample, ratios were obtained for dentin and cementum areas, and Pearson’s correlation coefficient was calculated. Ratios, which had a correlation coefficient greater than 0.40 were used for further statistical analysis. This led to selection of ratios only for dentin areas, and it allowed us to develop a regression formula. Partial Least Square (PLS) regression method was used for computing our model.

Results: A significant correlation was observed between the actual chronological age and predicted age of the individual using dentinal areas of the tooth. A closest to the estimated result was achieved, with an error of three years between predicted and actual chorological age.

Conclusion: Raman microspectrometry may be considered as an alternative to the conventional method of age estimation and contribute to the identification of individuals.

Keywords

Chronological age, Forensic dentistry, Forensic odontology

How to cite this article :

Karuna Kumari, Roopa S Rao, Sachin Chakradhar Sarode, Gargi Sachin Sarode, Shankargouda Patil. RAMAN MICROSPECTROMETRY: AN ALTERNATIVE METHOD OF AGE ESTIMATION FROM DENTIN AND CEMENTUM. Journal of Clinical and Diagnostic Research [serial online] 2017 October [cited: 2017 Oct 24 ]; 11:ZC11-ZC16. Available from
http://jcdr.net/back_issues.asp?issn=0973-709x&year=2017&month=October&volume=11&issue=10&page=ZC11-ZC16&id=10705

DOI and Others

DOI: 10.7860/JCDR/2017/31161.10705

Date of Submission: Jun 27, 2017
Date of Peer Review: Jul 22, 2017
Date of Acceptance: Aug 31, 2017
Date of Publishing: Oct 01, 2017

FINANCIAL OR OTHER COMPETING INTERESTS: None.

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