Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Original article / research
Year : 2017 | Month : September | Volume : 11 | Issue : 9 | Page : ZC28 - ZC32

Micronucleus Assay: An Early Diagnostic Tool to Assess Genotoxic Changes in Patients with Tobacco Use, Oral Leukoplakia and Oral Submucous Fibrosis

Moulshree Kohli, Puneet Ahuja, Monica Mehendiratta, Mohit Sharma, Jahnobi Dutta

1. Senior Lecturer, Department of Oral Pathology, I.T.S. Dental College, Hospital and Research Centre, Greater Noida, Uttar Pradesh, India. 2. Principal, Professor and Head, Department of Oral Pathology, I.T.S. Dental College, Hospital and Research Centre, Greater Noida, Uttar Pradesh, India. 3. Reader, Department of Oral Pathology, I.T.S. Dental College, Hospital and Research Centre, Greater Noida, Uttar Pradesh, India. 4. Reader, Department of Oral Pathology, I.T.S. Dental College, Hospital and Research Centre, Greater Noida, Uttar Pradesh, India. 5. Medical Research Scientist, Jorhat Medical College and Hospital, Barbheta, Jorhat, Assam, India.

Correspondence Address :
Dr. Moulshree Kohli,
House No.: 2008, Sector 37, Noida-201303, Uttar Pradesh, India.
E-mail: mauli.kohli@gmail.com

Abstract

Introduction: Micronuclei (MNi) are acentric chromatid or chromosome fragments produced via genetic damage through genotoxic agents contained in tobacco and betel nut. Evidently, the various Oral Potentially Malignant Disorders (OPMDs) like oral lichen Planus, oral leukoplakia and Oral Submucous Fibrosis (OSMF) demonstrate MNi, as a substantiation of genetic damage. As these changes can be easily appreciated in oral exfoliated cells, an exfoliated cell based MNi assay might be utilized as handy and non invasive biomonitoring tool for gauging the genetic damage and hence the propensity for malignant transformation in OPMDs. To this end, MNi are definitely easier to evaluate when compared to chromosome aberrations.

Aim: To compare the MNi frequency in normal mucosa, in individuals using various tobacco forms without oral leukoplakia, individuals using various tobacco forms with oral leukoplakia, and areca nut chewers with OSMF, using three different stains.

Materials and Methods: Oral exfoliated cells from 50 cases of normal mucosa (Group I), 50 cases of tobacco chewing people without Oral Leukoplakia (Group II), 50 cases of people with Oral Leukoplakia (Group III) and 50 cases of areca nut chewers with OSMF (Group IV) were taken. MNi frequencies were compared in these groups using three different stains i.e., Papanicolaou (PAP) stain, May Grunwald Giemsa (MGG) stain and Feulgen stain. The data between cases (Group II, III and IV) and control groups (Group I) was analyzed by Kruskal-Wallis Test. The comparison between two independent groups was done by Mann-Whitney U test and interstain comparison between cases and control was done by Wilcoxon Signed Rank Test and the individual p-value was obtained.

Results: A significant increase in the count was observed during transition of normal mucosa to OPMDs. The best stain for detecting MNi was PAP stain followed by MGG stain and Feulgen stain.

Conclusion: The higher mean MNi count for PAP stain and MGG stain could be attributed to nonspecific staining. Further study using a larger sample size on quantitative assessment of MNi count in various OPMDs is warranted.

Keywords

Carcinogenic, Chromatids, Disorders, Malignant, Papanicolaou

How to cite this article :

Moulshree Kohli, Puneet Ahuja, Monica Mehendiratta, Mohit Sharma, Jahnobi Dutta. MICRONUCLEUS ASSAY: AN EARLY DIAGNOSTIC TOOL TO ASSESS GENOTOXIC CHANGES IN PATIENTS WITH TOBACCO USE, ORAL LEUKOPLAKIA AND ORAL SUBMUCOUS FIBROSIS. Journal of Clinical and Diagnostic Research [serial online] 2017 September [cited: 2017 Nov 21 ]; 11:ZC28-ZC32. Available from
http://jcdr.net/back_issues.asp?issn=0973-709x&year=2017&month=September&volume=11&issue=9&page=ZC28-ZC32&id=10567

DOI and Others

DOI: 10.7860/JCDR/2017/27711.10567

Date of Submission: May 27, 2017
Date of Peer Review: Jun 16, 2017
Date of Acceptance: Jul 24, 2017
Date of Publishing: Sep 01, 2017

FINANCIAL OR OTHER COMPETING INTERESTS: None.

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