Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Original article / research
Year : 2018 | Month : August | Volume : 12 | Issue : 8 | Page : DC01 - DC05

Capsular Serotyping and Antimicrobial Drug Resistance of Klebsiella pneumoniae Strains Isolated from a Tertiary Care Hospital, Southern India

Lathamani Kotekani, Subbannayya Kotigadde, Bhaskaran Thulicheri Nair

1. Research Scholar, Department of Microbiology, Kvg Medical College, Sullia, Karnataka, India. 2. Professor, Department of Microbiology, Kvg Medical College, Sullia, Karnataka, India. 3. Associate Professor, Department of Microbiology, Kannur Medical College, Kannur, Kerala, India.

Correspondence Address :
Mrs. Lathamani Kotekani,
Research Scholar, Department of Microbiology, KVG Medical College, Sullia-574321, Karnataka, India.
E-mail: lathamani2006@rediffmail.com

Abstract

Introduction: The rapid spread of Hypervirulent Klebsiella pneumoniae (hvKP) and Multi Drug Resistant (MDR) strains among the clinical isolates of K.pneumoniae is a great deal of concern. The drug resistance and limited treatment options are the major threats. However, the spread of drug resistant strains is through plasmid which may carry virulence determinants such as capsular antigens.

Aim: To know the occurrence of hvKP; to study the occurrence of various capsular serotypes such as K1, K2, K3 and K4 among the isolates of K. pneumoniae using Co-agglutination (Co-A) test; to know the occurrence of beta lactamase genes (bla genes) among the K1, K2, K3 and K4 positive serotypes of K. pneumoniae. The study also tried to assess the reproducibility of Co-A test by comparing the result with PCR.

Materials and Methods: A prospective study was carried with a total of 60 consecutive, non-duplicate isolates of K. pneumoniae recovered from various clinical specimens obtained from both outpatients and inpatients between April to December 2017. To determine the occurrence of hvKP by string test, the identification of various serotypes was done by Co-A test and PCR. The determination of beta lactamase genes such as blaTEM, blaSHV and blaCTX-M among the capsular serotypes was done by PCR study.

Results: Rate of typability by Co-A was found to be 13(21.67%), 0(0%), 32(53.33%), 7(11.67%) for K1, K2, K3 and K4, respectively. Eight (13.33%) of them remained untypable. The rate of typability among the K. pneumoniae K1 serotype by Co-A test and PCR remained somewhat similar.

Conclusion: Co-A test is a good method of serotyping and can be used not only for diagnostic purpose but also for epidemiological study.

Keywords

bla genes, Hypermucoviscosity, K antigen, String test, Virulence

How to cite this article :

Lathamani Kotekani, Subbannayya Kotigadde, Bhaskaran Thulicheri Nair. CAPSULAR SEROTYPING AND ANTIMICROBIAL DRUG RESISTANCE OF KLEBSIELLA PNEUMONIAE STRAINS ISOLATED FROM A TERTIARY CARE HOSPITAL, SOUTHERN INDIA. Journal of Clinical and Diagnostic Research [serial online] 2018 August [cited: 2018 Jul 18 ]; 12:DC01-DC05. Available from
http://jcdr.net/back_issues.asp?issn=0973-709x&year=2018&month=August&volume=12&issue=8&page=DC01-DC05&id=11860

DOI and Others

DOI: 10.7860/JCDR/2018/35658.11860

Date of Submission: Jan 21, 2018
Date of Peer Review: Mar 26, 2018
Date of Acceptance: Apr 24, 2018
Date of Publishing: Aug 01, 2018

FINANCIAL OR OTHER COMPETING INTERESTS: None.

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