Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Original article / research
Year : 2018 | Month : July | Volume : 12 | Issue : 7 | Page : AC01 - AC04

Pathological and Biochemical Evaluation of Toxic Effects of Repeated Administration of Jasada Bhasma (Zinc Ash Formulation) in Wistar Albino Rats

Ravi Bhaskar, Megha A Doshi, Anjana Shidaraddi, Shakunthala Ramdas Pai, Naveen Kumar, Ravi Shankar, Ravi Mundugaru

1. PhD Scholar, Department of Anatomy, Krishna Institute of Medical Sciences, Deemed University, Karad, Maharashtra, India. 2. Professor and Head, Department of Anatomy, Krishna Institute of Medical Sciences, Deemed University, Karad, Maharashtra, India. 3. Tutor, Department of Anatomy, Srinivas Institute of Medical Sciences and Research Centre, Mukka, Mangaluru, Karnataka, India. 4. Professor and Head, Department of Anatomy, Kanachur Institute of Medical Sciences, Mangaluru, Karnataka, India 5. Assistant Professor, Department of Anatomy, Melaka Manipal Medical College, Manipal Academy of Higher Education, Manipal, Karnataka, India. 6. Director, Department of Pharmacology, SDM Research Centre of Ayurveda and Allied Sciences, Udupi, Karnataka, India. 7. Tutor, Department of Pharmacology, Kodagu Institute of Medical Sciences, Kodagu, Karnataka, India.

Correspondence Address :
Dr. Ravi Bhaskar,
PhD Scholar, Department of Anatomy, #401, Staff Quarters Srinivas Institute of Medical Sciences, Mukka, Mangaluru-575021, Karnataka, India.
E-mail: drravibhaskarr@gmail.com

Abstract

Introduction: Jasada Bhasma is the well known potent zinc formulation commonly used in ‘Ayurveda’ medicine system. Its extensive usage in the health care sector is proved to be successful in the treatment of certain illness and is considered to be popular in its stability, lower dose, and its availability.

Aim: To evaluate the toxicity profile of Jasada Bhasma with primary emphasis on haematological, biochemical and histopathological changes in Wistar albino rats.

Materials and Methods: For toxicity study, Wistar albino rats of either sex, weighing from 150-225 gm of body weight were used, and they were divided into three groups, ten rats in each group. Group-I administered with carboxymethyl cellulose and considered as vehicle control. Group-II and III were administered with two levels of Jasada Bhasma, i.e., 10.8 mg/kg (Therapeutic dose) and 54 mg/kg (Five times of therapeutic dose) respectively for 28 consecutive days. On the 28th day, the animals were sacrificed. Blood sample and vital organs were collected for haematological, biochemical and histopathological examinations.

Results: The repeated administration of Jasada Bhasma on Wistar albino rats did not produce remarkable toxicity changes at both the dose levels in most of the haematological parameters tested except there was a significant (p<0.01) increase in the mean cell haemoglobin concentration. Biochemically, the significant increase (p<0.01) in the serum creatinine but decrease in urea level has been shown in both levels of administration. The significant rise in potassium and total protein levels were also evident. Histopathologically, sections of liver and kidney showed pathological cytoarchitecture at both level dose exposures. However, sections of spleen showed mild to moderate increase in white pulp proportionate the five times of therapeutic dose level.

Conclusion: The repeated administration of test drug Jasada Bhasma at therapeutic dose was relatively safer; however, at higher dose level it produces some functional changes in histopathological sections of kidney and liver.

Keywords

Ayurveda, Histopathology, Toxicity

How to cite this article :

Ravi Bhaskar, Megha A Doshi, Anjana Shidaraddi, Shakunthala Ramdas Pai, Naveen Kumar, Ravi Shankar, et al.. PATHOLOGICAL AND BIOCHEMICAL EVALUATION OF TOXIC EFFECTS OF REPEATED ADMINISTRATION OF JASADA BHASMA (ZINC ASH FORMULATION) IN WISTAR ALBINO RATS. Journal of Clinical and Diagnostic Research [serial online] 2018 July [cited: 2018 Jul 23 ]; 12:AC01-AC04. Available from
http://jcdr.net/back_issues.asp?issn=0973-709x&year=2018&month=July&volume=12&issue=7&page=AC01-AC04&id=11755

DOI and Others

DOI: 10.7860/JCDR/2018/36665.11755

Date of Submission: Apr 06, 2018
Date of Peer Review: Apr 19, 2018
Date of Acceptance: Jun 01, 2018
Date of Publishing: Jul 01, 2018

FINANCIAL OR OTHER COMPETING INTERESTS: NONE.

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