Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Original article / research
Year : 2018 | Month : July | Volume : 12 | Issue : 7 | Page : BC26 - BC29

Role of D-Mannose as an Antiglycolytic Agent to Eliminate Preanalytical Error in Glucose Testing

Mithu Banerjee, Ankit Batra, Pratibha Misra

1. Professor, Department of Biochemistry, Armed Forces Medical College, Pune, Maharashtra, India. 2. Medical Officer, Department of Biochemistry, Armed Forces Medical College, Pune, Maharashtra, India. 3. Associate Professor, Department of Biochemistry, Armed Forces Medical College, Pune, Maharashtra, India.

Correspondence Address :
Dr. Pratibha Misra,
Associate Professor, Department of Biochemistry, Armed Forces Medical College, Pune-411040, Maharashtra, India.
E-mail: pratsghosh@gmail.com

Abstract

Introduction: Correct assessment of glucose is required for diagnosis of diabetes and monitoring of antihyperglycaemic therapy in adults as well monitoring neonatal hypoglycaemia. As per American Diabetes Association, sodium fluoride alone is an unreliable antiglycolytic agent in vitro due its ineffective action during first 4 hours of sample collection.

Aim: To evaluate the efficacy of mannose as compared to conventional antiglycolytic agent for blood glucose estimation and to assess its use as a preservative for other routine biochemical analytes.

Materials and Methods: The study was carried out in three steps. Fifteen samples of plasma were aliquoted into 5 parts. One was without any additive and in other four D-mannose was added in concentration of 1,2,3 and 5 mg/mL. Blood glucose was analysed immediately, at 1.5 hours, 3 hours and 5 hours to optimize the concentration of D-mannose. Fifteen serum samples were aliquoted into 2 parts, one without any additive and the other with D-mannose at a concentration of 3 mg/dL. Both were subjected for routine biochemistry to check for any interference by D-mannose in analysis. Fifteen samples were collected in heparin (with mannose added in concentration of 3mg/dL), plain tube, EDTA and sodium fluoride vacutainers. Blood glucose and other routine analytes were measured immediately, at 1.5 hrs and 3 hrs to compare D-mannose with other preservatives. Results were analysed using Studentís t-test.

Results: Optimal concentration of D-mannose was found to be of 3 mg/mL. D-Mannose showed a less decrease of blood glucose at the end of 3 hrs compared to sodium fluoride. D-Mannose does not interfere with estimation of any analytes except potassium. Creatine kinase was found to be lower with EDTA compared to other preservatives.

Conclusion: D-Mannose is an ideal preservative for blood glucose. All routine analytes (except potassium) can be estimated from a single vacutainer containing D-mannose at a concentration of 3 mg/dL.

Keywords

Glucose estimation, Glycolysis inhibitor, Glucose preservative

How to cite this article :

Mithu Banerjee, Ankit Batra, Pratibha Misra. ROLE OF D-MANNOSE AS AN ANTIGLYCOLYTIC AGENT TO ELIMINATE PREANALYTICAL ERROR IN GLUCOSE TESTING. Journal of Clinical and Diagnostic Research [serial online] 2018 July [cited: 2018 Jul 21 ]; 12:BC26-BC29. Available from
http://jcdr.net/back_issues.asp?issn=0973-709x&year=2018&month=July&volume=12&issue=7&page=BC26-BC29&id=11808

DOI and Others

DOI: 10.7860/JCDR/2018/35950.11808

Date of Submission: Feb 10, 2018
Date of Peer Review: Apr 12, 2018
Date of Acceptance: May 18, 2018
Date of Publishing: Jul 01, 2018

FINANCIAL OR OTHER COMPETING INTERESTS: NONE.

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