Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

Users Online : 39940

Original article / research
Year : 2018 | Month : June | Volume : 12 | Issue : 6 | Page : DC15 - DC19

Molecular Study of Aetiology of Acute Gastroenteritis in Children of South Mumbai

Seema Naresh Rohra, Vinay K Saxena, Neeru Praful Vithalani, Aruna Ananda Poojary, Tarique H I H Qureshi

1. Senior Clinical Microbiologist, Department of Pathology and Microbiology, Breach Candy Hospital Trust, Mumbai, Maharashtra, India. 2. Deputy Director, Department of Molecular Diagnostics, National Institute of Virology, Pune, Mumbai Unit, (formerly known as Enterovirus Research Centre), Maharashtra, India. 3. Consultant Paediatrician, Breach Candy Hospital Trust, Bhatia Hospital, SRCC Hospital and Saifee Hospital, Mumbai, Maharashtra, India. 4. Head of Department, Department of Pathology and Microbiology, Breach Candy Hospital Trust, Mumbai, Maharashtra, India. 5. Research Associate, Department of Molecular Diagnostics, National Institute of Virology, Pune, Mumbai Unit (formerly known as Enterovirus Research Centre), Maharashtra, India.

Correspondence Address :
Dr. Seema Naresh Rohra,
Department of Pathology and Microbiology, 2nd Floor, Annex wing, Breach Candy Hospital Trust, Mumbai-400026, Maharashtra, India.
E-mail: seema.kukreja@breachcandyhospital.org

Abstract

Introduction: Globally, Acute Gastroenteritis (AGE) is the leading cause of morbidity and mortality among children under five years of age. Majority of diarrhoeal illness in childhood are of viral aetiology. In the era of Rotavirus vaccine, Norovirus is emerging as an important cause of AGE in children.

Aim: To evaluate the aetiology of diarrhoea in children of South Mumbai.

Materials and Methods: A prospective study was carried out during July 2013 at a tertiary care hospital and research centre for evaluating diarrhoea in children =12 years of age. Clinical details of the patients were recorded on a case record form. Severity of disease was assessed by the modified Vesikari scoring pattern. Stool samples were collected from outpatients and inpatients. Stool routine microscopy and culture were done at tertiary care hospital. Aliquots of stool samples were tested at the Research Centre for Rota Virus, Adenovirus, Norovirus and Enterovirus by Polymerase Chain Reaction (PCR).

Results: A total of 51 patients were included in the study. Of these, 45.10% (23/51) were positive for viruses by real time PCR. Routine stool culture was positive for only one patient for E. coli O157. One patient had Entamoeba histolytica infection. Of 23 patients positive for viral aetiology, Norovirus was detected in 41.18% (21/51). Of the Norovirus positive children, 42.85% were =1 year of age. Diarrhoea followed by fever was the most common presentation. Among the Norovirus positive children, 33.33% (7/21) had moderately severe disease while 66.66 % (14/21) had mild disease.

Conclusion: Norovirus was the leading cause of acute gastroenteritis in the children included in the study. This study emphasizes the need to include Norovirus in the routine diagnostic algorithm of children with AGE and paves the way for syndromic approach based testing.

Keywords

Diarrhoea, India, Norovirus, Polymerase chain reaction

How to cite this article :

Seema Naresh Rohra, Vinay K Saxena, Neeru Praful Vithalani, Aruna Ananda Poojary, Tarique H I H Qureshi. MOLECULAR STUDY OF AETIOLOGY OF ACUTE GASTROENTERITIS IN CHILDREN OF SOUTH MUMBAI. Journal of Clinical and Diagnostic Research [serial online] 2018 June [cited: 2018 Jun 19 ]; 12:DC15-DC19. Available from
http://jcdr.net/back_issues.asp?issn=0973-709x&year=2018&month=June&volume=12&issue=6&page=DC15-DC19&id=11596

DOI and Others

DOI: 10.7860/JCDR/2018/35011.11596

Date of Submission: Nov 30, 2017
Date of Peer Review: Feb 15, 2018
Date of Acceptance: Apr 09, 2018
Date of Publishing: Jun 01, 2018

FINANCIAL OR OTHER COMPETING INTERESTS: None.

JCDR is now Monthly and more widely Indexed .
  • Emerging Sources Citation Index (Web of Science, thomsonreuters)
  • Index Copernicus ICV 2016: 132.37
  • SCOPUS
  • Academic Search Complete Database
  • Directory of Open Access Journals (DOAJ)
  • EBSCOhost
  • Embase & EMbiology
  • Google Scholar
  • HINARI Access to Research in Health Programme
  • Indian Science Abstracts (ISA)
  • Journal seek Database
  • Google
  • Popline (reproductive health literature)
  • www.omnimedicalsearch.com