Correlation of the Probiotic Cell Number ml-1 and the Cell Mediated Immune Response: an in Vitro Study
Dr. Aruna Bhatia, Dean Life Sciences, Department of Biotechnology, Punjabi University, Patiala-147002, Punjab, India. Phone: 91-9878263077, Fax: 910-175-2286682 E-mail: firstname.lastname@example.org
The beneficial effects of probiotics have been related to their survival number in the gut. But on the other hand, the dead probiotics also show immunostimulation and antioxidant activity. Hence, this study was planned to evaluate the in vitro, cell mediated immune response of six probiotic strains by differing their cell number ml-1 viz. 1 x 106 cell ml-1 and 1 x 109 cell ml-1 by using pig splenocytes. Splenocytes which were incubated with the six strains separately at concentrations of 1 x 106 cell ml-1 and 1 x 109 cell ml-1 for 24 hours, were assayed to study the cell mediated immune response by employing the Nitroblue Tetrazolium Reduction test and the Inducible Nitric Oxide Synthase test and by studying the bactericidal activity. The results demonstrated that a substantial increase in the stimulation of the cells occurred by the effect of all the probiotic strains at both the concentrations i.e. 1 x 106 cell ml-1 and 1 x 109 cell ml-1.However, the stimulation of the splenocytes was invariably higher at the concentration of 1 x 109 cell ml-1.The study suggests that higher number of cells should be employed during animal experimentation with probiotics to get observable effects.