Characterisation of Multidrug-resistant Alcaligenes faecalis Strain AF1 Isolated from Patient of RUTIs: A Study from BangladeshCorrespondence Address :
Md Javed Foysal,
PhD Research Fellow, School of Molecular and Life Sciences, Curtin University, WA-6845, Perth, WA, Australia.
E-mail: firstname.lastname@example.org; email@example.com
Introduction: Alcaligenes faecalis is a common environmental bacterium that often infects human as an opportunistic pathogen. It rarely causes Urinary Tract Infection (UTI) in human; however, infection brings severe outcomes. Also, the treatment of A. faecalis associated infections with common antibiotics can be difficult due to a high level of antibiotic resistance.
Aim: Molecular characterisation of A. faecalis isolate from women suffering from Recurrent Urinary Tract Infections (RUTIs).
Materials and Methods: The study was conducted in the USDA-laboratory of the Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet, Bangladesh. The present study conducted from July 2017 to December 2017, characterised an A. faecalis strain from the women suffering from RUTIs by applying 16s rRNA gene sequencing and phylogenetic analysis. Antibiogram profiling was done by means of disk diffusion assay. Analysis of in-silico SNP variants was done using Geneious software.
Results: The isolates were resistant to seven out of ten commercial antibiotics used to treat UTI in Bangladesh. Phylogenetic and evolutionary distance data analysis revealed a close proximity of the study AF1 strain with other A. faecalis strains identified from the environment, especially from a previously characterised water sample. In-silico variants search found nine potentials of Single Nucleotide Polymorphism (SNPs) in the studied strain compared to other environmental A. faecalis bacteria characterised from India, South-Korea, Japan, Mexico and Brazil.
Conclusion: The present study revealed the transmission of environmental opportunistic pathogens to human and cause chronic infections in Bangladesh.
16s rRNA gene sequencing, Multidrug-resistant strain, Phylogenetic analysis
Farhana Momtaz, Md Hazrat Ali, Md Nazmul Hossain, Md Javed Foysal, Mariya Kibtiya Sumiya, Kamrul Islam. CHARACTERISATION OF MULTIDRUG-RESISTANT ALCALIGENES FAECALIS STRAIN AF1 ISOLATED FROM PATIENT OF RUTIS: A STUDY FROM BANGLADESH. Journal of Clinical and Diagnostic Research [serial online] 2018 October [cited: 2019 Jan 24 ]; 12:KC01-KC04. Available from
Date of Submission: May 23, 2018
Date of Peer Review: Jul 05, 2018
Date of Acceptance: Jul 27, 2018
Date of Publishing: Oct 01, 2018
FINANCIAL OR OTHER COMPETING INTERESTS: None.
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