Nucleic Acid Amplification Testing Detects HIV Transmission Risk in Serologically-Tested Blood Donor UnitsCorrespondence Address :
Dr. Isaac Dadzie,
Department of Medical Laboratory Science, School of Allied Health Sciences, University of Cape Coast, Cape Coast, Ghana.
Introduction: Blood transfusion is an essential life-saving intervention in the healthcare delivery. Mandatory screening of donor units helps prevent transfusion-transmissible infections, such as Human Immunodeficiency Virus (HIV).
Aim: The purpose of this study was to use Nucleic Acid-Amplification Testing (NAAT) to screen for the presence of HIV-1 in blood-banked samples labelled as “safe for transfusion” per serological testing algorithm.
Materials and Methods: This hospital-based cross-sectional diagnostic study was conducted in May 2016 on serologically tested donor blood units in the Koforidua Regional Hospital blood bank. One hundred (100) donor samples were analysed using the COBAS® AmpliPrep/COBAS® TaqMan® HIV-1 Qual Test for the detection of HIV-1 RNA and proviral DNA.
Results: Of the 100 donor samples that tested sero-negative for HIV-1 and 2 using the antibody screening kit, four (4) samples (4%) were reactive by NAAT. Blood donors who came for donation were only males and majority (90%) were between the ages of 17-27 years. Additionally, all the NAAT HIV-1 positive samples were from participants in the 17-27 year group.
Conclusion: NAAT demonstrated that a significant number of HIV-infected individuals are misdiagnosed at Ghanaian points-of-care. This finding has necessitated the need for inclusion of NAAT in donor blood screening in areas prevalent for HIV-1 in Ghana, considering the risk involved in using the licensed antibody test provided by the health authorities. In cases where NAAT screening may not be feasible, newer tests that have greater sensitivity compared to the FDA-licensed 3rd generation EIA which only detects HIV antibodies can be adopted.
Blood safety, HIV diagnosis, Transfusion transmissible infections
Isaac Dadzie, Shemau Muniru, Patatrick Adu, Obed Cudjoe. NUCLEIC ACID AMPLIFICATION TESTING DETECTS HIV TRANSMISSION RISK IN SEROLOGICALLY-TESTED BLOOD DONOR UNITS. Journal of Clinical and Diagnostic Research [serial online] 2018 September [cited: 2018 Sep 24 ]; 12:DC22-DC24. Available from
Date of Submission: Feb 23, 2018
Date of Peer Review: Apr 12, 2018
Date of Acceptance: Jun 21, 2018
Date of Publishing: Sep 01, 2018
Financial OR OTHER COMPETING INTERESTS: None.
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