Alternative Rapid Methods for Coverslip Removal: A Comparative StudyCorrespondence Address :
Mr. Sachin Shivaji Kapse,
Postgraduate, Department of Pathology, B.L.D.E University, Shri. B.M. Patil Medical College, Vijayapura-586103, Karnataka, India.
Introduction: In 21st century, the focus is to develop method which yields outcome in shorter tenure, and are rapid, safe and obtain quicker results. In this study, non-traditional methods were used for removal of coverslip and was compared with traditional methods.
Aim: To compare the time taken for coverslip removal of old faded slides with five different methods.
Materials and Methods:Faded slides of one year to more than 10-year-old were included in the study. Total of 90 slides were subjected to five different methods of coverslip removal like xylene at room temperature, xylene at 56° Celsius (C), Petrol, Diesel and Freezing method. Time taken for each method was noted.
Results: The mean time taken for removal of coverslip was least with freezing method followed by xylene at 560C, Petrol, xylene at room temperature and diesel.
Conclusion: Alternative methods are safe, rapid and has less turn around time compared to routine use of xylene. Hence, can be successfully used for removal of coverslip.
Freezing, Petrol, Restaining, Xylene
Mahesh H Karigoudar, Sachin Shivaji Kapse, Disha B Shankar, Shikha Sharma, Divya Prafulla Yerraguntla. ALTERNATIVE RAPID METHODS FOR COVERSLIP REMOVAL: A COMPARATIVE STUDY. Journal of Clinical and Diagnostic Research [serial online] 2019 January [cited: 2019 Jan 23 ]; 13:EC01-EC02. Available from
Date of Submission: May 31, 2018
Date of Peer Review: Jul 24, 2018
Date of Acceptance: Nov 08, 2018
Date of Publishing: Jan 01, 2019
FINANCIAL OR OTHER COMPETING INTERESTS: None.
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