Antimicrobial Efficacy of Garlic-Lemon in Comparison with Sodium Hypochlorite against E. faecalisCorrespondence Address :
Dr. K Anjaneyulu,
Senior Lecturer, Department of Conservative Dentistry and Endodontics, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, 162, Poonamallee High Road, Chennai-600077, Tamil Nadu, India.
Introduction: Sodium hypochlorite is the most commonly used root canal irrigant in endodontics. Although, it is a highly effective antimicrobial agent, it is highly cytotoxic. This study has focused on search of alternative herbal irrigants which is more biocompatible.
Aim: To evaluate antimicrobial efficacy of ethanolic garlic-lemon and fresh garlic-lemon mixture in comparison with 2.5% sodium hypochlorite against E. faecalis at different intervals (first, third and sixth day).
Materials and Methods: Extracts were divided into three groups: Group 1-ethanolic garlic-lemon extract, Group 2-fresh garlic-lemon and Group 3-2.5% sodium hypochlorite. The antimicrobial activity was determined using agar diffusion test. Zone of inhibition of bacterial growth was recorded for one, three and six days. Statistical analysis was performed using one-way Anova and post-hoc Bonferroni test.
Results: On first day, sodium hypochlorite showed maximum inhibitory effect compared to ethanol and fresh garlic-lemon extract. On third day, ethanolic extract of garlic-lemon showed significant maximum inhibitory effect followed by sodium hypochlorite and fresh garlic-lemon extract. On sixth day, ethanolic extract of garlic-lemon showed significant inhibitory effect followed by fresh garlic-lemon extract and sodium hypochlorite.
Conclusion: In this study, herbal extract has showed increased antimicrobial efficacy when compared to sodium hypochlorite. Ethanolic extract has showed maximum inhibitory effect compared to fresh garlic-lemon and sodium hypochlorite.
Agar diffusion test, Ethanolic extract, Ethanolic extract of garlic-lemon, Fresh garlic-lemon
Riluwan Siddique, K Anjaneyulu, NP Muralidharan. ANTIMICROBIAL EFFICACY OF GARLIC-LEMON IN COMPARISON WITH SODIUM HYPOCHLORITE AGAINST E. FAECALIS. Journal of Clinical and Diagnostic Research [serial online] 2019 January [cited: 2019 Jan 22 ]; 13:ZC55-ZC58. Available from
Date of Submission: Jul 02, 2018
Date of Peer Review: Aug 16, 2018
Date of Acceptance: Nov 14, 2018
Date of Publishing: Jan 01, 2019
FINANCIAL OR OTHER COMPETING INTERESTS: None.
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