Ex Vivo Evaluation of the Erosive Potential of Typical Fruit Juices from Brazilian Tropical ForestsCorrespondence Address :
Nádia Rezende Barbosa Raposo,
Rua José Lourenço Kelmer, s/n, Campus Universitário, Juiz de Fora, Minas Gerais, Brazil.
Introduction: Dental erosion is defined as an irreversible loss of hard tissue caused by intrinsic and extrinsic acidic sources, non-bacterial dissolution not directly associated with traumatic factors or tooth decay.
Aim: The aim of this study was to analyse the ex-vivo pH, titratable acidity and erosive potential of following juices: Theobroma grandiflorum, Annona muricata, Spondias mombin and Spondias tuberosa, which are commonly found and widely used fruits by the population of the northern and north-eastern regions of Brazil.
Materials and Methods: Fifteen human third molars, extracted for clinical reasons and obtained from Human Teeth Bank of the Dental School (Federal University of Juiz de Fora), were selected to be immersed in the juices and in 1% citric acid (positive control group) and perform erosive cycles. The samples were immersed in the solutions for 2 minute, five times a day, at intervals of two hour, for four days. Between the erosive cycles, the specimens remained in artificial saliva. Before the beginning of the erosive cycles and at the end of the cycle sequence, specimens were analysed for roughness and for micro-hardness. Statistical analyses were performed using the IBM SPSS Statistics 22.0 software. The results were expressed as a mean±standard error of the mean, and analysed using variance analysis (ANOVA), followed by post-hoc Tukey’s test. The significance accepted was p<0.05.
Results: The pH of all juices analysed was acidic, within a narrow range of pH values from 2.41 to 3.28 and titratable acidity values suggest that the beverages tested have pH lower than the critical pH for dental enamel.
Conclusion: The data analysis allows one to conclude that all the juices presented acid pH and promoted significant loss of superficial enamel hardness. Regarding the surface roughness of the samples from this study, only 1% citric acid and the juice of Theobroma grandiflorum reached values close to or greater than the critical surface roughness. Therefore, one must be aware of the great erosive potential of these juices when drinking them.
Acids, Adverse effects, Fruit and vegetable juices, Hydrogen-ion concentration, Tooth demineralization, Tooth erosion
Renée Mazilão de Paula, Régis de Souza Apolinário, Isadora Conde Ferreira Martins, Hanny Reis Mockdeci Gonçalves, José Luiz Fernandes Vieira, Maria Das Graças Afonso Miranda Chaves, et al.. EX VIVO EVALUATION OF THE EROSIVE POTENTIAL OF TYPICAL FRUIT JUICES FROM BRAZILIAN TROPICAL FORESTS. Journal of Clinical and Diagnostic Research [serial online] 2019 July [cited: 2019 Jul 23 ]; 13:ZC36-ZC40. Available from
Date of Submission: Mar 09, 2019
Date of Peer Review: Mar 19, 2019
Date of Acceptance: May 16, 2019
Date of Publishing: Jul 01, 2019
FINANCIAL OR OTHER COMPETING INTERESTS: None.
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