Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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On Sep 2018




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On Sep 2018




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"Journal of Clinical and Diagnostic Research is at present a well-known Indian originated scientific journal which started with a humble beginning. I have been associated with this journal since many years. I appreciate the Editor, Dr. Hemant Jain, for his constant effort in bringing up this journal to the present status right from the scratch. The journal is multidisciplinary. It encourages in publishing the scientific articles from postgraduates and also the beginners who start their career. At the same time the journal also caters for the high quality articles from specialty and super-specialty researchers. Hence it provides a platform for the scientist and researchers to publish. The other aspect of it is, the readers get the information regarding the most recent developments in science which can be used for teaching, research, treating patients and to some extent take preventive measures against certain diseases. The journal is contributing immensely to the society at national and international level."



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Lucknow
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On Aug 2018




Dr. Arundhathi. S
"Journal of Clinical and Diagnostic Research (JCDR) is a reputed peer reviewed journal and is constantly involved in publishing high quality research articles related to medicine. Its been a great pleasure to be associated with this esteemed journal as a reviewer and as an author for a couple of years. The editorial board consists of many dedicated and reputed experts as its members and they are doing an appreciable work in guiding budding researchers. JCDR is doing a commendable job in scientific research by promoting excellent quality research & review articles and case reports & series. The reviewers provide appropriate suggestions that improve the quality of articles. I strongly recommend my fraternity to encourage JCDR by contributing their valuable research work in this widely accepted, user friendly journal. I hope my collaboration with JCDR will continue for a long time".



Dr. Arundhathi. S
MBBS, MD (Pathology),
Sanjay Gandhi institute of trauma and orthopedics,
Bengaluru.
On Aug 2018




Dr. Mamta Gupta,
"It gives me great pleasure to be associated with JCDR, since last 2-3 years. Since then I have authored, co-authored and reviewed about 25 articles in JCDR. I thank JCDR for giving me an opportunity to improve my own skills as an author and a reviewer.
It 's a multispecialty journal, publishing high quality articles. It gives a platform to the authors to publish their research work which can be available for everyone across the globe to read. The best thing about JCDR is that the full articles of all medical specialties are available as pdf/html for reading free of cost or without institutional subscription, which is not there for other journals. For those who have problem in writing manuscript or do statistical work, JCDR comes for their rescue.
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Reviewing articles is no less a pain staking process and requires in depth perception, knowledge about the topic for review. It requires time and concentration, yet I enjoy doing it. The JCDR website especially for the reviewers is quite user friendly. My suggestions for improving the journal is, more strict review process, so that only high quality articles are published. I find a a good number of articles in Obst. Gynae, hence, a new journal for this specialty titled JCDR-OG can be started. May be a bimonthly or quarterly publication to begin with. Only selected articles should find a place in it.
An yearly reward for the best article authored can also incentivize the authors. Though the process of finding the best article will be not be very easy. I do not know how reviewing process can be improved. If an article is being reviewed by two reviewers, then opinion of one can be communicated to the other or the final opinion of the editor can be communicated to the reviewer if requested for. This will help one’s reviewing skills.
My best wishes to Dr. Hemant Jain and all the editorial staff of JCDR for their untiring efforts to bring out this journal. I strongly recommend medical fraternity to publish their valuable research work in this esteemed journal, JCDR".



Dr. Mamta Gupta
Consultant
(Ex HOD Obs &Gynae, Hindu Rao Hospital and associated NDMC Medical College, Delhi)
Aug 2018




Dr. Rajendra Kumar Ghritlaharey

"I wish to thank Dr. Hemant Jain, Editor-in-Chief Journal of Clinical and Diagnostic Research (JCDR), for asking me to write up few words.
Writing is the representation of language in a textual medium i e; into the words and sentences on paper. Quality medical manuscript writing in particular, demands not only a high-quality research, but also requires accurate and concise communication of findings and conclusions, with adherence to particular journal guidelines. In medical field whether working in teaching, private, or in corporate institution, everyone wants to excel in his / her own field and get recognised by making manuscripts publication.


Authors are the souls of any journal, and deserve much respect. To publish a journal manuscripts are needed from authors. Authors have a great responsibility for producing facts of their work in terms of number and results truthfully and an individual honesty is expected from authors in this regards. Both ways its true "No authors-No manuscripts-No journals" and "No journals–No manuscripts–No authors". Reviewing a manuscript is also a very responsible and important task of any peer-reviewed journal and to be taken seriously. It needs knowledge on the subject, sincerity, honesty and determination. Although the process of reviewing a manuscript is a time consuming task butit is expected to give one's best remarks within the time frame of the journal.
Salient features of the JCDR: It is a biomedical, multidisciplinary (including all medical and dental specialities), e-journal, with wide scope and extensive author support. At the same time, a free text of manuscript is available in HTML and PDF format. There is fast growing authorship and readership with JCDR as this can be judged by the number of articles published in it i e; in Feb 2007 of its first issue, it contained 5 articles only, and now in its recent volume published in April 2011, it contained 67 manuscripts. This e-journal is fulfilling the commitments and objectives sincerely, (as stated by Editor-in-chief in his preface to first edition) i e; to encourage physicians through the internet, especially from the developing countries who witness a spectrum of disease and acquire a wealth of knowledge to publish their experiences to benefit the medical community in patients care. I also feel that many of us have work of substance, newer ideas, adequate clinical materials but poor in medical writing and hesitation to submit the work and need help. JCDR provides authors help in this regards.
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In the era of fast growing newer technologies, and in computer and internet friendly environment the manuscripts preparation, submission, review, revision, etc and all can be done and checked with a click from all corer of the world, at any time. Of course there is always a scope for improvement in every field and none is perfect. To progress, one needs to identify the areas of one's weakness and to strengthen them.
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Every one of us: authors, reviewers, editors, and publisher are responsible for enhancing the stature of the journal. I wish for a great success for JCDR."



Thanking you
With sincere regards
Dr. Rajendra Kumar Ghritlaharey, M.S., M. Ch., FAIS
Associate Professor,
Department of Paediatric Surgery, Gandhi Medical College & Associated
Kamla Nehru & Hamidia Hospitals Bhopal, Madhya Pradesh 462 001 (India)
E-mail: drrajendrak1@rediffmail.com
On May 11,2011




Dr. Shankar P.R.

"On looking back through my Gmail archives after being requested by the journal to write a short editorial about my experiences of publishing with the Journal of Clinical and Diagnostic Research (JCDR), I came across an e-mail from Dr. Hemant Jain, Editor, in March 2007, which introduced the new electronic journal. The main features of the journal which were outlined in the e-mail were extensive author support, cash rewards, the peer review process, and other salient features of the journal.
Over a span of over four years, we (I and my colleagues) have published around 25 articles in the journal. In this editorial, I plan to briefly discuss my experiences of publishing with JCDR and the strengths of the journal and to finally address the areas for improvement.
My experiences of publishing with JCDR: Overall, my experiences of publishing withJCDR have been positive. The best point about the journal is that it responds to queries from the author. This may seem to be simple and not too much to ask for, but unfortunately, many journals in the subcontinent and from many developing countries do not respond or they respond with a long delay to the queries from the authors 1. The reasons could be many, including lack of optimal secretarial and other support. Another problem with many journals is the slowness of the review process. Editorial processing and peer review can take anywhere between a year to two years with some journals. Also, some journals do not keep the contributors informed about the progress of the review process. Due to the long review process, the articles can lose their relevance and topicality. A major benefit with JCDR is the timeliness and promptness of its response. In Dr Jain's e-mail which was sent to me in 2007, before the introduction of the Pre-publishing system, he had stated that he had received my submission and that he would get back to me within seven days and he did!
Most of the manuscripts are published within 3 to 4 months of their submission if they are found to be suitable after the review process. JCDR is published bimonthly and the accepted articles were usually published in the next issue. Recently, due to the increased volume of the submissions, the review process has become slower and it ?? Section can take from 4 to 6 months for the articles to be reviewed. The journal has an extensive author support system and it has recently introduced a paid expedited review process. The journal also mentions the average time for processing the manuscript under different submission systems - regular submission and expedited review.
Strengths of the journal: The journal has an online first facility in which the accepted manuscripts may be published on the website before being included in a regular issue of the journal. This cuts down the time between their acceptance and the publication. The journal is indexed in many databases, though not in PubMed. The editorial board should now take steps to index the journal in PubMed. The journal has a system of notifying readers through e-mail when a new issue is released. Also, the articles are available in both the HTML and the PDF formats. I especially like the new and colorful page format of the journal. Also, the access statistics of the articles are available. The prepublication and the manuscript tracking system are also helpful for the authors.
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Overall, the publishing process with JCDR has been smooth, quick and relatively hassle free and I can recommend other authors to consider the journal as an outlet for their work."



Dr. P. Ravi Shankar
KIST Medical College, P.O. Box 14142, Kathmandu, Nepal.
E-mail: ravi.dr.shankar@gmail.com
On April 2011
Anuradha

Dear team JCDR, I would like to thank you for the very professional and polite service provided by everyone at JCDR. While i have been in the field of writing and editing for sometime, this has been my first attempt in publishing a scientific paper.Thank you for hand-holding me through the process.


Dr. Anuradha
E-mail: anuradha2nittur@gmail.com
On Jan 2020

Important Notice

Original article / research
Year : 2021 | Month : August | Volume : 15 | Issue : 8 | Page : SC09 - SC12 Full Version

Paediatric Blood Lead Levels and its Correlation with Anaemia: A Study from Central India


Published: August 1, 2021 | DOI: https://doi.org/10.7860/JCDR/2021/48930.15297
Usha Banga, Pawan Kumar Sharma, Jyoti Gupta, Ashok Banga, Puneet Agrawal, Abhishek Mehta, Rajesh Gupta

1. Paediatric Consultant, Aastha Clinic, Gwalior, Madhya Pradesh, India. 2. Associate Professor, Department of Paediatrics, Government Medical College, Datia, Madhya Pradesh, India. 3. Assistant Professor, Department of Microbiology, BMHRC, Bhopal, Madhya Pradesh, India. 4. Paediatric Consultant, Aastha Clinic, Gwalior, Madhya Pradesh, India. 5. Senior Resident, Department of Paediatrics Government Medical College, Datia, Madhya Pradesh, India. 6. Associate Professor, Department of Microbiology, Government Medical College, Datia, Madhya Pradesh, India. 7. Professor, Department of Paediatrics Government Medical College, Datia, Madhya Pradesh, India.

Correspondence Address :
Dr. Rajesh Gupta,
723, Near District Industrial Center, Civil Lines, Datia, Madhya Pradesh, India.
E-mail: drrajesh93@gmail.com

Abstract

Introduction: Lead is a toxic element used commonly in many products like lead-acid batteries, paints, toys etc. Young children are particularly vulnerable to lead poisoning because of higher absorption capacity in comparison of adults. Safe level of lead exposure has to be established yet. Literature suggests a threshold lead level of 20-40 μg/dL for a decrease in haemoglobin in children.

Aim: To assess the correlation between Blood Lead Levels (BLL) and anaemia in children.

Materials and Methods: This cross-sectional study was conducted in a Paediatric Tertiary Care Hospital of Gwalior, Madhya Pradesh, India, during the period of May 2019 to September 2020. A 81 children between one to five years of age were included in study. Information was collected regarding general profile of the children and other parameters like socio-economic status (Modified Kuppuswamy scale), history of pica, application of surma, type of toys used, scholastic performance and somatic symptoms. Routine blood investigations were done including Haemoglobin (Hb) and BLL through venous blood sampling. BLL were done at Defense Research and Development Establishment (DRDE) Gwalior. Data entry was done in Microsoft Office Excel 2010 and analysed using Statistical Package for the Social Sciences (SPSS) software version 21.0. Chi-square test with Yates correlation was applied and p-value <0.05 was considered statistically significant.

Results: Among 81 study subjects, 53 were males and 28 were females. Total 48 (59.2%) children had Hb <11 gm/dL, 42 (51.8%) children had BLL <5 μg/dL and 32 (39.5%) had BLL between 5-10 μg/dL. There were 9% children (n=7) with BLL more than 10 μg/dL and none beyond 15 μg/dL. In the children it was found to have BLL ≥5 μg/dL, 20 children had Hb levels between 7-11 gm/dL whereas 19 had Hb levels above 11 gm/dL. No statistically significant association was found between the BLL and childhood anaemia.

Conclusion: This study did not show any relationship between BLL and Hb in children of age one to five years. Scarcity of diagnostic tools for detecting BLL and lack of public awareness regarding the probable sources of lead and its complications may result in missing the diagnosis.

Keywords

Children, Development, Haemoglobin

Lead is one of the most common toxic elements of environmental origin. It is widely used in many products such as lead-acid batteries, pigments, paints, solder, stained glass, lead crystal glassware, ammunition, ceramic glazes, jewellery, and toys and in some cosmetics and traditional medicines (1). From a particular source, young children absorb more lead as compared with adults (2). Scientific evidences show that BLLs even below 10 μg/dL are associated with adverse cognitive, behavioural, attention problems, language difficulties and other toxic effects in infants and children [3,4]. Identification of lead poisoning is difficult at an early stage as the symptoms are non specific and mimic that of the other disorders, thus patients receive only symptomatic treatment (5). Under-nourished children absorb more lead, making them more susceptible (2). No safe level of lead exposure has so far been established (1).

The Centre for Disease Control (CDC) and the American Academy of Paediatrics (AAP) currently use the reference value of 5 μg/dL instead of the previous level of ≥10 μg/dL in community settings after recommendation by an expert committee in January 2012 on the basis of neurological toxicity (6),(7),(8). It has been revealed by a number of studies that more than half the children in India have BLL ≥10 μg/dL
(9),(10). Elevated BLL ≥10 μg/dL have been observed and found significantly associated with moderate and severe anaemia after accounting for other factors influencing anaemia (11). According to National Family Health Survey (NFHS)-4 (2015-16) 58.6% Indian children between age 6 to 59 months are anaemic (Hb <11 g/dL) (12). The Environmental Protection Agency has suggested a threshold lead level of 20-40 μg/dL for a decrease in Hb in children, although a clear cut-off is not defined and needs further studies to support through data (13). This study has been conducted to assess any correlation between BLL and paediatric anaemia along with considering impact of various socio-demographic, economic and other factors on lead poisoning.

Material and Methods

This cross-sectional study was conducted in a private Paediatric Tertiary Care Hospital at Gwalior, Madhya Pradesh, India, during the period of May 2019 to September 2020. The study followed all the ethical principles for medical research involving human subjects, according to the Declaration of Helsinki.

Inclusion criteria: Children between one to five years of age who visited the study clinic during the study period and were managed on outdoor basis were included in this study.

Exclusion criteria: Children falling out of age criteria, diagnosed with haemolytic anaemia or any acute illness requiring admission and those unwilling to participate in the study were excluded from the study.

Study Procedure

Total 81 children showing clinical signs of anaemia were recruited as study participants by simple random sampling after obtaining informed written consent from their parents. Information was collected over a predesigned peer-reviewed proforma regarding general profile of the child and other parameters like socio-economic status based on modified Kuppuswamy scale (14), history of pica, application of surma, type of toys used, scholastic performance, somatic symptoms like tiredness, headache, decreased play activity, decreased appetite, abdominal pain, behavioural symptoms like restlessness, destructive activities, violent behaviour, irritability, stealing, bullying, thumb sucking, type of house paint and use of cosmetics. Cronbach’s alpha value for this questionnaire was 0.741. All study subjects went through general and systemic examination including anthropometry. Routine blood investigations were done including Hb and BLL through venous blood sampling. BLLs were done at DRDE, Gwalior.

Statistical Analysis

Data entry was done in MS office excel 2010 and analysed using SPSS software version 21.0 (trial version). Simple Linear Regression analysis was done for correlation between Hb and BLL. Chi-square test of independence with Yates correction was applied. The p-value <0.05 was considered statistically significant.

Results

Among 81 study subjects, 54 (66.7%) were males and 28 (33.3%)were females. Among all the children, 28 (34.5%) were from rural areas in comparison to 53 (65.5%) urban children. About one third (34%) of the studied children belonged to lower middle and upper lower category of socio-economic class (Chi-square test of independence with Yates correction; p-value: 0.049491). Total 48 (59.2%) children had anaemia as per World Health Organisation (WHO) cut off for anaemia i.e., Hb <11 gm/dL. 18 (22.2%) children had a history of pica, however 65 (80.2%) had a history of regular application of Surma and 37% of them had BLL ≥5 μg/dL (Chi-square test of independence with Yates correction; p-value: 0.000641). Majority (56) of the children were regularly exposed to plastic toys. Parents were asked to talk about their children’s school performance and 13 (16%) admitted their poor performance and majority (68%) were unaware about it. Relation of selected baseline characteristics to BLL in studied children has been shown in (Table/Fig 1).

A 42 (52%) children had BLLs <5 μg/dL and 32 (40%) had BLLs between 5-10 μg/dL, 7 (8.6%) children had BLLs more than 10 μg/dL and none beyond 15 μg/dL. In the children found to have BLLs ≥5 μg/dL, 20 (48.8%) children had Hb levels between 7-11 gm/dL
whereas 19 had Hb levels above 11 gm/dL. None of the studied child had severe anaemia i.e., Hb <7 gm/dL. As per the observations of this study, no statistically significant association was found between the BLL and childhood anaemia (Table/Fig 2). Linear regression analysis for Hb levels of studied children in relation to their BLLs have been plotted in (Table/Fig 3), which suggests no obvious relationship of Hb levels with BLLs. Relation of selected clinical characteristics to BLL in the studied children has been shown in (Table/Fig 4). Tiredness, decreased appetite, irritability, restlessness/hyper activity were significant findings among children with BLL ≥5 μg/dL.

Discussion

This study was done to estimate BLL in children and to study its correlation with various attributes including Hb. Total 49% of children under this study were found with BLLs greater than or equal to 5 μg/dL which is in accordance with the findings of previous similar studies done at Jammu, New Delhi and Nagpur. Cut-off value of BLL for these studies was 10 μg/dL (9),(10). In the present study, like the previous ones from Delhi and Mumbai, demographic variables such as education and gender did not associate with BLL (15).

In an Indian study, after adjustment for child’s age, breastfeeding duration, standard of living, parent’s education and occupation, maternal anaemia and number of children in the immediate family, children with lead levels >10 μg/dL were 1.3 times as likely to have moderate anaemia as children with lead levels <10 μg/dL. Similarly, the odds ratio for severe anaemia was 1.7 (11). Present study does not indicate any association between BLL and childhood anaemia.

Another study conducted among 12 primary schools in Chennai tested the hypothesis that Transferrin (TF) C2 polymorphism modifies the effects of lead and Hb on intelligence and found a significant interaction between lead and Hb with the C2 variant. Children with TF C2 variant are more prone to the neurotoxic effects of lead while being less protected by higher levels of Hb (16).

In another similar study done at Delhi and Mumbai, no association was found between anaemia and BLL in Delhi (p=0.6) however statistically significant association was found between the two parameters in Mumbai (p=0.01). Cut off value of BLL for these studies was 10 μg/dL. Authors concluded that in such a situation, it may be difficult to say whether iron deficiency anaemia is linked to higher lead levels or not (17).

A low standard of living correlated with a 32.3% increase in BLLs (15). Lead levels >10 μg/dL have been observed significantly associated with increasing age, duration of breastfeeding, a lower standard of living, a low educational status of parents and a greater number of siblings (11). The stronger associations between cognition and cortical volume in children from low income families living in high lead risk tracts suggest that children can be greatly benefited from environmental adversity with reductions in environmental insults associated with lead exposure risk (18). About one third of the index study subjects belonged to lower middle and upper lower category of socio-economic class as per Modified Kuppuswamy scale having BLL ≥5 μg/dL.

Literature suggests that young children are more vulnerable to lead poisoning due to higher capacity to absorb ingested lead from a given source than adults (2). Their innate curiosity results in their mouthing and swallowing lead containing or lead coated objects. This psychological disorder called pica is magnified in children, who may, for example pick away at, and eat, leaded paint from walls, door frames and furniture. In Nigeria, Senegal and, other countries, exposure to lead contaminated soil and dust resulting from battery recycling and mining has caused mass lead poisoning and multiple deaths in young children (19). In the present study, only 27% children had a history of pica out of which only 12% had BLL ≥5 μg/dL.

Surma, which is an ore that is mined and ground into a powder, being used for centuries as a cosmetic and to ward off evil in children as well as women. In the absence of manufacturing regulation, its lead content varies greatly, from 16-70% and traditionally surma use in eyes of children has been a popular practice in India (20). A study was conducted in Kolkata with objective of regular surma application in eyes of children and its association with BLLs and anaemia. Same study also assessed lead content of different types of surma and found high lead levels in all samples of surma except the white one (out of total 34 samples of black, brown, orange, grey and white varieties of surma). The mean blood lead concentration of 69 surma user children was found to be 29.6±10.2 μg/dL whereas non user’s value was 4.9±0.8 μg/dL. Low Hb levels were also observed in the users (10.2±1.4 g/100 mL) (21). In present study, three-fourth of the children had a history of surma application in eyes regularly and 37% of them had BLLs ≥5 μg/dL which is highly significant (p-value 0.000641).

Majority of the studied children were exposed to plastic toys and 25 (44.6%) of them had BLL greater than or equal to 5 μg/dL. In a community based cross-sectional study of 297 children aged six months to six years in Nagpur, the authors assessed the prevalence of elevated (>10 μg/dL) BLL, their risk factors, and the lead contents in potential environmental sources. Analysis of various environmental sources such as paint, pencils, crayons, and clay revealed high lead levels and prevalence of elevated BLL in exposed subjects was observed as 67%. The results of such studies demonstrate the existence of a major environmental health problem among Indian children (10). However, in the present study, no statistically significant association of exposure to plastic toys and BLLs in children was identified.

Symptoms of lead intoxication may be subtle and mimic other disorders. The general symptoms of lead poisoning include abdominal pain, vomiting, weakness, dizziness, body aches, loss of appetite, anxiety, encephalopathy, dementia, seizures, and coma (5). Present study observed and analysed symptoms like tiredness, headache, decreased play activity, decreased appetite, abdominal pain, restlessness, hyperactivity, destructive activity, irritability, violent behaviour and thumb sucking. Sufficient evidences have supported that BLL even <5 μg/dL in children are associated with increased diagnosis of attention related behavioural problems and decreased cognitive performance as indicated by lower academic achievement, decreased Intelligence Quotient (IQ), and reductions in specific cognitive measures (22).

Limitation(s)

This study was done involving children attending paediatric clinic for minor illnesses. Due to time and resource constraints subjects could not be assessed for other toxic effects of raised lead levels like IQ and developmental anomalies. The findings are affected by variability and hence cannot be applied for the entire population from which the sample is drawn. In future the authors would like to extend this research further at institutional level or in the field involving larger population that could give more generalisable findings for the entire population.

Conclusion

This study did not show any relationship between BLL and Hb in one to five years children. However, 49% of studied children attending routine outdoor clinic have been observed with BLLs ≥5 μg/dL which is an alarming situation in a resource limited country. Scarcity of diagnostic tools for detecting BLL and lack of public awareness regarding the probable sources of lead and its complications may lead in overlooking lead poisoning. Data needs to be procured through further studies to identify serious consequences of lead poisoning in children, particularly affecting their brain development and nervous system. This data will help in developing strategy regarding appropriate therapeutic interventions in affected population.

Acknowledgement

Defence Research and Development Establishment, Gwalior, Ministry of Defence, Government of India for processing blood samples to procure BLL. Dr. Shubhanshu Gupta, Assistant Professor, Community Medicine, GMC Datia for support in statistical analysis.

References

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DOI and Others

10.7860/JCDR/2021/48930.15297

Date of Submission: Feb 09, 2021
Date of Peer Review: Apr 24, 2021
Date of Acceptance: Jul 06, 2021
Date of Publishing: Aug 01, 2021

AUTHOR DECLARATION:
• Financial or Other Competing Interests: None
• Was Ethics Committee Approval obtained for this study? No
• Was informed consent obtained from the subjects involved in the study? Yes
• For any images presented appropriate consent has been obtained from the subjects. No

PLAGIARISM CHECKING METHODS:
• Plagiarism X-checker: Jul 01, 2021
• Manual Googling: Jul 03, 2021
• iThenticate Software: Jul 23, 2021 (23%)

ETYMOLOGY: Author Origin

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