Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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"Journal of Clinical and Diagnostic Research is at present a well-known Indian originated scientific journal which started with a humble beginning. I have been associated with this journal since many years. I appreciate the Editor, Dr. Hemant Jain, for his constant effort in bringing up this journal to the present status right from the scratch. The journal is multidisciplinary. It encourages in publishing the scientific articles from postgraduates and also the beginners who start their career. At the same time the journal also caters for the high quality articles from specialty and super-specialty researchers. Hence it provides a platform for the scientist and researchers to publish. The other aspect of it is, the readers get the information regarding the most recent developments in science which can be used for teaching, research, treating patients and to some extent take preventive measures against certain diseases. The journal is contributing immensely to the society at national and international level."



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Lucknow
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Dr. Arundhathi. S
MBBS, MD (Pathology),
Sanjay Gandhi institute of trauma and orthopedics,
Bengaluru.
On Aug 2018




Dr. Mamta Gupta,
"It gives me great pleasure to be associated with JCDR, since last 2-3 years. Since then I have authored, co-authored and reviewed about 25 articles in JCDR. I thank JCDR for giving me an opportunity to improve my own skills as an author and a reviewer.
It 's a multispecialty journal, publishing high quality articles. It gives a platform to the authors to publish their research work which can be available for everyone across the globe to read. The best thing about JCDR is that the full articles of all medical specialties are available as pdf/html for reading free of cost or without institutional subscription, which is not there for other journals. For those who have problem in writing manuscript or do statistical work, JCDR comes for their rescue.
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Reviewing articles is no less a pain staking process and requires in depth perception, knowledge about the topic for review. It requires time and concentration, yet I enjoy doing it. The JCDR website especially for the reviewers is quite user friendly. My suggestions for improving the journal is, more strict review process, so that only high quality articles are published. I find a a good number of articles in Obst. Gynae, hence, a new journal for this specialty titled JCDR-OG can be started. May be a bimonthly or quarterly publication to begin with. Only selected articles should find a place in it.
An yearly reward for the best article authored can also incentivize the authors. Though the process of finding the best article will be not be very easy. I do not know how reviewing process can be improved. If an article is being reviewed by two reviewers, then opinion of one can be communicated to the other or the final opinion of the editor can be communicated to the reviewer if requested for. This will help one’s reviewing skills.
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Dr. Mamta Gupta
Consultant
(Ex HOD Obs &Gynae, Hindu Rao Hospital and associated NDMC Medical College, Delhi)
Aug 2018




Dr. Rajendra Kumar Ghritlaharey

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Writing is the representation of language in a textual medium i e; into the words and sentences on paper. Quality medical manuscript writing in particular, demands not only a high-quality research, but also requires accurate and concise communication of findings and conclusions, with adherence to particular journal guidelines. In medical field whether working in teaching, private, or in corporate institution, everyone wants to excel in his / her own field and get recognised by making manuscripts publication.


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Salient features of the JCDR: It is a biomedical, multidisciplinary (including all medical and dental specialities), e-journal, with wide scope and extensive author support. At the same time, a free text of manuscript is available in HTML and PDF format. There is fast growing authorship and readership with JCDR as this can be judged by the number of articles published in it i e; in Feb 2007 of its first issue, it contained 5 articles only, and now in its recent volume published in April 2011, it contained 67 manuscripts. This e-journal is fulfilling the commitments and objectives sincerely, (as stated by Editor-in-chief in his preface to first edition) i e; to encourage physicians through the internet, especially from the developing countries who witness a spectrum of disease and acquire a wealth of knowledge to publish their experiences to benefit the medical community in patients care. I also feel that many of us have work of substance, newer ideas, adequate clinical materials but poor in medical writing and hesitation to submit the work and need help. JCDR provides authors help in this regards.
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Indexation and online availability: Indexation transforms the journal in some sense from its local ownership to the worldwide professional community and to the public.JCDR is indexed with Embase & EMbiology, Google Scholar, Index Copernicus, Chemical Abstracts Service, Journal seek Database, Indian Science Abstracts, to name few of them. Manuscriptspublished in JCDR are available on major search engines ie; google, yahoo, msn.
In the era of fast growing newer technologies, and in computer and internet friendly environment the manuscripts preparation, submission, review, revision, etc and all can be done and checked with a click from all corer of the world, at any time. Of course there is always a scope for improvement in every field and none is perfect. To progress, one needs to identify the areas of one's weakness and to strengthen them.
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Every one of us: authors, reviewers, editors, and publisher are responsible for enhancing the stature of the journal. I wish for a great success for JCDR."



Thanking you
With sincere regards
Dr. Rajendra Kumar Ghritlaharey, M.S., M. Ch., FAIS
Associate Professor,
Department of Paediatric Surgery, Gandhi Medical College & Associated
Kamla Nehru & Hamidia Hospitals Bhopal, Madhya Pradesh 462 001 (India)
E-mail: drrajendrak1@rediffmail.com
On May 11,2011




Dr. Shankar P.R.

"On looking back through my Gmail archives after being requested by the journal to write a short editorial about my experiences of publishing with the Journal of Clinical and Diagnostic Research (JCDR), I came across an e-mail from Dr. Hemant Jain, Editor, in March 2007, which introduced the new electronic journal. The main features of the journal which were outlined in the e-mail were extensive author support, cash rewards, the peer review process, and other salient features of the journal.
Over a span of over four years, we (I and my colleagues) have published around 25 articles in the journal. In this editorial, I plan to briefly discuss my experiences of publishing with JCDR and the strengths of the journal and to finally address the areas for improvement.
My experiences of publishing with JCDR: Overall, my experiences of publishing withJCDR have been positive. The best point about the journal is that it responds to queries from the author. This may seem to be simple and not too much to ask for, but unfortunately, many journals in the subcontinent and from many developing countries do not respond or they respond with a long delay to the queries from the authors 1. The reasons could be many, including lack of optimal secretarial and other support. Another problem with many journals is the slowness of the review process. Editorial processing and peer review can take anywhere between a year to two years with some journals. Also, some journals do not keep the contributors informed about the progress of the review process. Due to the long review process, the articles can lose their relevance and topicality. A major benefit with JCDR is the timeliness and promptness of its response. In Dr Jain's e-mail which was sent to me in 2007, before the introduction of the Pre-publishing system, he had stated that he had received my submission and that he would get back to me within seven days and he did!
Most of the manuscripts are published within 3 to 4 months of their submission if they are found to be suitable after the review process. JCDR is published bimonthly and the accepted articles were usually published in the next issue. Recently, due to the increased volume of the submissions, the review process has become slower and it ?? Section can take from 4 to 6 months for the articles to be reviewed. The journal has an extensive author support system and it has recently introduced a paid expedited review process. The journal also mentions the average time for processing the manuscript under different submission systems - regular submission and expedited review.
Strengths of the journal: The journal has an online first facility in which the accepted manuscripts may be published on the website before being included in a regular issue of the journal. This cuts down the time between their acceptance and the publication. The journal is indexed in many databases, though not in PubMed. The editorial board should now take steps to index the journal in PubMed. The journal has a system of notifying readers through e-mail when a new issue is released. Also, the articles are available in both the HTML and the PDF formats. I especially like the new and colorful page format of the journal. Also, the access statistics of the articles are available. The prepublication and the manuscript tracking system are also helpful for the authors.
Areas for improvement: In certain cases, I felt that the peer review process of the manuscripts was not up to international standards and that it should be strengthened. Also, the number of manuscripts in an issue is high and it may be difficult for readers to go through all of them. The journal can consider tightening of the peer review process and increasing the quality standards for the acceptance of the manuscripts. I faced occasional problems with the online manuscript submission (Pre-publishing) system, which have to be addressed.
Overall, the publishing process with JCDR has been smooth, quick and relatively hassle free and I can recommend other authors to consider the journal as an outlet for their work."



Dr. P. Ravi Shankar
KIST Medical College, P.O. Box 14142, Kathmandu, Nepal.
E-mail: ravi.dr.shankar@gmail.com
On April 2011
Anuradha

Dear team JCDR, I would like to thank you for the very professional and polite service provided by everyone at JCDR. While i have been in the field of writing and editing for sometime, this has been my first attempt in publishing a scientific paper.Thank you for hand-holding me through the process.


Dr. Anuradha
E-mail: anuradha2nittur@gmail.com
On Jan 2020

Important Notice

Original article / research
Year : 2022 | Month : November | Volume : 16 | Issue : 11 | Page : EC27 - EC31 Full Version

Histomorphological Changes in Pancreas and Liver among Chronic AlcoholicsAn Autopsy Study


Published: November 1, 2022 | DOI: https://doi.org/10.7860/JCDR/2022/53684.17145
Danish S Shaikh, Shailesh Vartak, Urmi Vartak

1. Speciality Medical Officer, Department of Pathology, Lokmanya Tilak Municipal Medical College, Mumbai, Maharashtra, India. 2. Associate Professor, Department of Pathology, Lokmanya Tilak Municipal Medical College, Mumbai, Maharashtra, India. 3. Associate Professor, Department of Pathology, Lokmanya Tilak Municipal Medical College, Mumbai, Maharashtra, India.

Correspondence Address :
Shailesh Vartak,
Associate Professor, Department of Pathology, Lokmanya Tilak Municipal Medical College, Mumbai, Maharashtra, India.
E-mail: shailvar@gmail.com

Abstract

Introduction: The clinical and pathological association between pancreatitis and alcohol abuse is well recognised, however the concurrence of prevalence of alcoholic related pancreatitis and liver disease is less well studied.

Aim: To evaluate frequency of histomorphological changes in pancreas and liver among patients with history of alcohol abuse and observe the prevalence of co-existence between chronic pancreatitis and liver cirrhosis.

Materials and Methods: This was observational cross-sectional study conducted in Department of Pathology at Lokmanya Tilak Municipal Medical College, Mumbai, Maharashtra, India, from July 2013 to July 2018. The study included 1917 autopsies and 107 cases with a documented history of chronic alcohol abuse. Haematoxylin and Eosin (H&E) staining was done on all sections and special stain like Masson’s trichrome was performed as per
indication. Gross and microscopy were studied under variable defined parameters. Data was entered in Microsoft Excel sheets.

Results: Histomorphologically, 12 cases (11.21%) were diagnosed as pancreatitis; 10 cases (9.34%) were of acute pancreatitis and 2 (1.86%) cases were of chronic pancreatitis. Total 21 cases were diagnosed as liver cirrhosis. The most dominant pattern of fibrosis seen in pancreatitis was perilobular and interlobular periductal fibrosis. The frequency of pancreatitis (14.28%) and pancreatic fibrosis (38.09%) was found to be more in cirrhotics. Chronic pancreatitis was commonly seen in cirrhotics than in non cirrhotics. Similarly, liver cirrhosis was more commonly observed in cases of chronic pancreatitis.

Conclusion: The frequency of histomorphological changes seen in pancreas and liver was observed considerably among patients giving history of alcohol abuse. The prevalence of co-existence of chronic pancreatitis and liver cirrhosis was 50%.

Keywords

Alcoholic cirrhosis, Interlobular periductal fibrosis, Perilobular fibrosis, Stellate cells, Steatosis

Liver disease and pancreatic changes are two conditions that commonly co-occur in chronic alcoholics. The clinical and pathological association between pancreatitis and alcohol abuse is well recognised, however the concurrence of prevalence and association of alcoholic related pancreatitis and liver disease is less well studied. Both liver and pancreatic alcoholic diseases have precursor lesions which bear association with one another.

The development of liver cirrhosis requires history of several years of chronic alcoholism (1),(2),(3). For many years, it was considered that around 10-35% of subjects with chronic alcoholism developed alcoholic liver cirrhosis (4). More recently, prospective studies have shown that the frequency is indeed much lower in alcoholics i.e, around 2% (1),(2). The stimulation of stellate cells as an initiating event in development
of parenchymal fibrosis in chronic alcoholics, follows a similar process in both liver and pancreas (1).

The aim of the present study was to evaluate effects of chronic alcoholism on pancreas and liver in patients who gave a history of chronic alcohol abuse by examining the histological changes in pancreas and liver; and observe the prevalence of co-existence between chronic pancreatitis and liver cirrhosis as well as their precursor lesions.

Material and Methods

This was observational cross-sectional study conducted in Department of Pathology at Lokmanya Tilak Municipal Medical College, Mumbai, Maharashtra, India, from July 2013 to July 2018 (three years retrospective and two years prospective). The study conducted was reviewed by Institutional Ethics Committee (1/03/2017) at the Tertiary Care Hospital. The study was unanimously approved. Consent was obtained by the institute from the relatives of the patient, prior to autopsy.

Inclusion criteria: Out of the total 1917 cases in the duration of five years, all cases with a documented history of chronic alcohol abuse i.e 107 patients were included in the study.

Exclusion criteria: Alcoholic liver disease cases with documentation of positive antigens or antibodies against hepatitis B or C virus were excluded from the study. Liver showing findings of tuberculous necrosis, malignancies, liver abscesses and any other primary liver lesion not associated with chronic alcoholism were considered as confounding factors. Similarly, pancreatitis caused by other etiological factors like smoking, gall stones, hypertriglyceridemia and abdominal surgeries without history of chronic alcoholism were excluded from the study.

The basic data was collected from the autopsy record i.e, gender, age at death, cause of death and relevant clinical history and autopsy findings.

Procedure

The organs (liver, pancreas, heart, lungs) were dissected at the time of autopsy following appropriate protocols and obtaining consent. After dissection they were stored in formalin in the Autopsy Histopathology Department. Following adequate formalin fixation, representative tissue sections were taken from the various organs. The tissues were processed in tissue processor, and paraffin blocks were prepared. This was followed by staining with Haematoxylin and Eosin (H&E) stains and slides were prepared.

Histological parameters studied in pancreas: Fibrosis (diffuse fibrosis, perilobular fibrosis, intralobular fibrosis and interlobular periductal fibrosis), parenchymal necrosis and haemorrhage, fat necrosis and peripancreatic soft tissue necrosis, inspissated eosinophilic ductal secretions, fibrin thrombi, lobular atrophy, parenchymal calcification, fat infiltration and neutrophilic/lymphoplasmacytic infiltration.

Histological parameters studied: Microvesicular and macrovesicular steatosis, ballooning degeneration, Mallory hyaline/Mallory Denk bodies, hepatocyte necrosis, portal tract and lobular inflammation, fibrosis in
portal tract including bridging (portal to portal/central to portal) fibrosis, cholestasis and bile duct proliferation.

The special stain Masson’s Trichrome was performed in all the cases where fibrosis was observed on H&E slide. Tissue from lung and uterus were used as positive control.

Staining method: Following were the reagents used in staining:

• Wiegert’s Iron Haematoxylin solution: This was freshly prepared by mixing Haematoxylin stock solution and Ferric chloride stock solution (prepared fresh).

• Biebrich Scarlet-Acid fuchsin solution (1% aqueous Biebrich Scarlet 90 mL+1% aqueous acid fuchsin 10 mL+glacial acetic acid 1 mL)

• Phosphomolybdic- phosphotungstic acid solution (5% phosphomolybdic acid 25 mL+5% phosphotungstic acid 25 mL).

• Aniline blue solution (aniline blue 2.5 g+glacial acetic acid 2 mL+distilled water 100 mL).

• 1% Acetic acid solution (glacial acetic acid 1 mL+distilled water 99 mL) for differentiation.

• Bouin’s solution (for improved staining quality).

Wiegert’s Haematoxylin is resistant to decolourisation by acidic staining solutions and stains the nuclei blue. Biebrich scarlet-acid fuchsin stains all the acidic tissues like the cytoplasm, muscle, and collagen. Phosphomolybdic or phosphotungstic acid is a decolourising agent, making the scarlet-acid fuchsin diffuse out of the collagen fibres. Thus, the muscle fibres stain red. Aniline blue stains the collagen along which 1% acetic acid which is added for differentiation. The collagen fibres stain blue and the nuclei stain black, with a red background. This principle was used to detect presence, amount and distribution of fibrosis of fibrosis. Because of background staining, trichrome stains also allow easy evaluation of liver architecture.

Statistical analysis

Data was entered in Microsoft Excel sheets. The prevalence or percentage was calculated from the findings of the study.

Results

There were 107 patients (106 males and 1 female) who had presented with a definite history of chronic alcohol abuse. Age ranged from 20 to 80 years with a mean of 49.39 years. Of the included cases, 6 (5.6%) died of heart disease. In 22 (20.56%) cases, the cause of death was stated as due to alcoholic liver disease. Total 7 (6.54%) cases died due to pancreatitis. Majority of the cases died i.e., 72 cases (67.2%), were of pulmonary diseases. Cirrhosis of micronodular type was documented in 12 cases (11.21%) and macronodular type was documented in 6 cases (5.60%) whereas, 89 cases (83.17%) were non cirrhotic. Grossly visible haemorrhagic pancreatitis was observed in six cases; changes of chronic pancreatitis in the form of firm and fibrotic pancreas which were slightly shrunken were documented in one case.

There were altogether 12 cases (11.21%) which were diagnosed as pancreatitis on histology, of which 10 cases (9.34%) were of acute pancreatitis and 2 (1.86%) cases were diagnosed as chronic pancreatitis (Table/Fig 1). Grossly visible liver parenchymal necrosis was documented in 5 (4.67%) cases giving history of alcohol abuse (does not exclude tuberculosis caseous necrosis and liver abscess) and fatty change was documented in 15 (14.01%) cases. The
two dominant pattern of fibrosis seen in acute pancreatitis (n=10) were perilobular (n=3) and interlobular periductal (n=3), which was followed by intralobular fibrosis (n=2). No evidence of diffuse fibrosis pattern was visible in cases of acute pancreatitis. Similarly, the most dominant pattern of fibrosis in chronic pancreatitis (n=2) was diffuse fibrosis which was evident in both the cases (one case of chronic pancreatitis showed more than one pattern of fibrosis). The most dominant pattern of fibrosis seen in acute pancreatitis was perilobular and that in chronic pancreatitis was diffuse fibrosis. The frequency of pancreatitis was found to be more in cirrhotics (14.28%) than in non cirrhotics (10.46%). Chronic pancreatitis was found to be more prevalent in cirrhotics (4.76%) than in non cirrhotics (1.1%). Similarly, the frequency of pancreatic fibrosis was more in cirrhotics (38.09%), than in non cirrhotics (24.4%) [Table/Fig-(2),(3),(4).

Of the two cases diagnosed as chronic pancreatitis, hepatic macrovesicular steatosis was demonstrated in one case and Mallory hyaline was evident in one of the cases. Of these two cases of chronic pancreatitis, cirrhosis was seen in one case and steatosis (macrovesicular) was seen in another case. Of the total 21 cases of liver cirrhosis, 2 (9.5%) were of acute pancreatitis and 1 (4.76%) case was of chronic pancreatitis. Similarly, of the 29 cases of pancreatic fibrosis, liver cirrhosis was seen in 8 (27.5%) cases and steatosis was seen in 16 (55.17%) cases (Table/Fig 5).

The prevalence of co-existence of chronic pancreatitis and liver cirrhosis was 50%.

Discussion

Many investigators have explained the involved mechanisms in the co-occurrence of liver diseases and pancreatitis. Alcohol consumption has marked and specific effects on the liver and pancreas, as evidenced by the existence of disease categories (5),(6),(7),(8). Despite the strong association between excessive alcohol consumption and development of chronic pancreatitis, alcohol alone is not sufficient to lead to the disease. Only a small proportion of chronic alcoholics (5-10%) develop chronic pancreatitis (Table/Fig 6) (9),(10),(11).

A retrospective study by Griener L et al., on 112 chronic alcoholics using endoscopic retrograde pancreatography and liver biopsy showed that 65 patients were diagnosed with chronic pancreatitis (58.03%) and 30.7% (20/65 cases) suffered from an additional alcoholic liver cirrhosis (9). Thus, data shows higher susceptibility for liver disease than for pancreatic diseases in alcoholics. Cases of cirrhosis in alcoholics in the present study were more prevalent than cases of pancreatitis, thereby indicating that liver damage in alcoholics is more common than pancreatic damage (Table/Fig 7) (10),(11),(12).

A review of 1022 autopsy cases (Renner IG et al., in 1984) with alcoholic liver cirrhosis showed a prevalence of chronic pancreatitis and pancreatic fibrosis of 20% [Table/Fig-(8),(9) (10),(11),(12),(13).

In a study by Kochhar R et al., on 46 patients with alcoholic liver disease, 43.4% patients had features of chronic pancreatitis on endoscopic retrograde pancreatogram; but there was no difference in the prevalence of pancreatic changes in cirrhotic in comparison to non cirrhotic patients (14). Caradonna P et al., (n=60), found only prevalence of 7% chronic pancreatitis in patients with alcoholic liver cirrhosis (15). The hepatic and pancreatic stellate cells play a central role in the development of fibrosis in both the liver and the pancreas, implying similar molecular mechanisms underlying the development of fibrotic changes in both liver and pancreas (11),(16),(17). Stellate cells have been studied more in detail in the pathogenesis of fibrotic process of chronic alcohol related injuries in liver and pancreas (Table/Fig 10) (11),(18),(19),(20).

Stellate cells are believed to be activated directly by alcohol and its metabolites and also by cytokines and growth factors released during alcohol-induced pancreatic and hepatic necrosis and inflammation. This activated state of stellate cell is the main source of extracellular matrix production and collagenisation (17),(18). Gullo L et al., (1995) found an incidence of liver cirrhosis diagnosed by liver biopsy of 14% in 50 patients with chronic pancreatitis (Table/Fig 11) (11),(21),(22). In a study by Pace A et al., high prevalence of pancreatic injury was also observed in association with the precursor of liver cirrhosis like severe hepatic steatosis (11). Chronic pancreatitis and liver disease both have precursor lesions. Recent studies show that chronic pancreatitis develops through fibrosis of the pancreatic parenchyma while the precursor form of liver cirrhosis is hepatic steatosis [11,23].

A study by Zsori G et al., demonstrated the presence of liver fibrosis through the use of transient elastography in around one-third (33%) of alcoholic chronic pancreatitis patients (24).

The quantity of consumed alcohol and the presence of diabetes mellitus are risk factors for the development of liver fibrosis in alcoholic chronic pancreatitis. The hepatic and pancreatic stellate cells play a central role in the development of fibrosis both in the liver and in the pancreas, implying similar molecular mechanisms underlying the development of fibrotic changes in these organs (16),(17). The two conditions were found to be closely associated. Moreover, the precursor of cirrhosis and chronic pancreatitis, steatosis and pancreatic fibrosis are commonly found in the same individual, suggesting common risk factors being predominant. The above study identified a very high prevalence of both diseases in alcoholics. The results of the current study are in contrast with several studies that have evaluated the relationship between chronic pancreatitis and liver cirrhosis. The microscopic pictures of variouslesions stained with H&E and Masson’s trichrome special stain are listed [Table/Fig-(12),(13),(14),(15),(16),(17),(18),(19),(20),(21),(22).

Limitation(s)

One of the main limitations of the study was that there was no information about the exact quantity of daily alcohol intake, or the exact duration of drinking. History of smoking and nutritional profiles were not taken into consideration due to lack of documentation in the history sheet.

Conclusion

In conclusion, the prevalence of chronic pancreatitis were more in cirrhotics than in non cirrhotics. The simultaneous occurrence or co-existence of chronic pancreatitis and liver cirrhosis caused by alcohol consumption in the same individual, were also closely associated. The prevalence of co-existence of chronic pancreatitis and liver cirrhosis was 50%. Moreover, the precursor lesions of chronic pancreatitis and liver cirrhosis, pancreatic fibrosis and steatosis, are frequently observed in the same individual, suggesting a predominance of common risk factors.

References

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Becker U, Deis A, Sorensen T, Gronbaek M, Borch-Johnsen K, Muller CF, et al. Prediction of risk of liver disease by alcohol intake, sex, and age: A prospective population study. Hepatology. 1996;23(5):1025-29.[crossref] [PubMed]
2.
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DOI and Others

DOI: 10.7860/JCDR/2022/53684.17145

Date of Submission: Jan 03, 2022
Date of Peer Review: Mar 02, 2022
Date of Acceptance: Apr 06, 2022
Date of Publishing: Nov 01, 2022

Author declaration:
• Financial or Other Competing Interests: None
• Was Ethics Committee Approval obtained for this study? Yes
• Was informed consent obtained from the subjects involved in the study? Yes
• For any images presented appropriate consent has been obtained from the subjects. Yes

PLAGIARISM CHECKING METHODS:
• Plagiarism X-checker: Jan 12, 2022
• Manual Googling: Jan 18, 2022
• iThenticate Software: Apr 01, 2022 (21%)

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