Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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On Sep 2018




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On Aug 2018




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Dr. Arundhathi. S
MBBS, MD (Pathology),
Sanjay Gandhi institute of trauma and orthopedics,
Bengaluru.
On Aug 2018




Dr. Mamta Gupta,
"It gives me great pleasure to be associated with JCDR, since last 2-3 years. Since then I have authored, co-authored and reviewed about 25 articles in JCDR. I thank JCDR for giving me an opportunity to improve my own skills as an author and a reviewer.
It 's a multispecialty journal, publishing high quality articles. It gives a platform to the authors to publish their research work which can be available for everyone across the globe to read. The best thing about JCDR is that the full articles of all medical specialties are available as pdf/html for reading free of cost or without institutional subscription, which is not there for other journals. For those who have problem in writing manuscript or do statistical work, JCDR comes for their rescue.
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Reviewing articles is no less a pain staking process and requires in depth perception, knowledge about the topic for review. It requires time and concentration, yet I enjoy doing it. The JCDR website especially for the reviewers is quite user friendly. My suggestions for improving the journal is, more strict review process, so that only high quality articles are published. I find a a good number of articles in Obst. Gynae, hence, a new journal for this specialty titled JCDR-OG can be started. May be a bimonthly or quarterly publication to begin with. Only selected articles should find a place in it.
An yearly reward for the best article authored can also incentivize the authors. Though the process of finding the best article will be not be very easy. I do not know how reviewing process can be improved. If an article is being reviewed by two reviewers, then opinion of one can be communicated to the other or the final opinion of the editor can be communicated to the reviewer if requested for. This will help one’s reviewing skills.
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Dr. Mamta Gupta
Consultant
(Ex HOD Obs &Gynae, Hindu Rao Hospital and associated NDMC Medical College, Delhi)
Aug 2018




Dr. Rajendra Kumar Ghritlaharey

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Writing is the representation of language in a textual medium i e; into the words and sentences on paper. Quality medical manuscript writing in particular, demands not only a high-quality research, but also requires accurate and concise communication of findings and conclusions, with adherence to particular journal guidelines. In medical field whether working in teaching, private, or in corporate institution, everyone wants to excel in his / her own field and get recognised by making manuscripts publication.


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Salient features of the JCDR: It is a biomedical, multidisciplinary (including all medical and dental specialities), e-journal, with wide scope and extensive author support. At the same time, a free text of manuscript is available in HTML and PDF format. There is fast growing authorship and readership with JCDR as this can be judged by the number of articles published in it i e; in Feb 2007 of its first issue, it contained 5 articles only, and now in its recent volume published in April 2011, it contained 67 manuscripts. This e-journal is fulfilling the commitments and objectives sincerely, (as stated by Editor-in-chief in his preface to first edition) i e; to encourage physicians through the internet, especially from the developing countries who witness a spectrum of disease and acquire a wealth of knowledge to publish their experiences to benefit the medical community in patients care. I also feel that many of us have work of substance, newer ideas, adequate clinical materials but poor in medical writing and hesitation to submit the work and need help. JCDR provides authors help in this regards.
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Every one of us: authors, reviewers, editors, and publisher are responsible for enhancing the stature of the journal. I wish for a great success for JCDR."



Thanking you
With sincere regards
Dr. Rajendra Kumar Ghritlaharey, M.S., M. Ch., FAIS
Associate Professor,
Department of Paediatric Surgery, Gandhi Medical College & Associated
Kamla Nehru & Hamidia Hospitals Bhopal, Madhya Pradesh 462 001 (India)
E-mail: drrajendrak1@rediffmail.com
On May 11,2011




Dr. Shankar P.R.

"On looking back through my Gmail archives after being requested by the journal to write a short editorial about my experiences of publishing with the Journal of Clinical and Diagnostic Research (JCDR), I came across an e-mail from Dr. Hemant Jain, Editor, in March 2007, which introduced the new electronic journal. The main features of the journal which were outlined in the e-mail were extensive author support, cash rewards, the peer review process, and other salient features of the journal.
Over a span of over four years, we (I and my colleagues) have published around 25 articles in the journal. In this editorial, I plan to briefly discuss my experiences of publishing with JCDR and the strengths of the journal and to finally address the areas for improvement.
My experiences of publishing with JCDR: Overall, my experiences of publishing withJCDR have been positive. The best point about the journal is that it responds to queries from the author. This may seem to be simple and not too much to ask for, but unfortunately, many journals in the subcontinent and from many developing countries do not respond or they respond with a long delay to the queries from the authors 1. The reasons could be many, including lack of optimal secretarial and other support. Another problem with many journals is the slowness of the review process. Editorial processing and peer review can take anywhere between a year to two years with some journals. Also, some journals do not keep the contributors informed about the progress of the review process. Due to the long review process, the articles can lose their relevance and topicality. A major benefit with JCDR is the timeliness and promptness of its response. In Dr Jain's e-mail which was sent to me in 2007, before the introduction of the Pre-publishing system, he had stated that he had received my submission and that he would get back to me within seven days and he did!
Most of the manuscripts are published within 3 to 4 months of their submission if they are found to be suitable after the review process. JCDR is published bimonthly and the accepted articles were usually published in the next issue. Recently, due to the increased volume of the submissions, the review process has become slower and it ?? Section can take from 4 to 6 months for the articles to be reviewed. The journal has an extensive author support system and it has recently introduced a paid expedited review process. The journal also mentions the average time for processing the manuscript under different submission systems - regular submission and expedited review.
Strengths of the journal: The journal has an online first facility in which the accepted manuscripts may be published on the website before being included in a regular issue of the journal. This cuts down the time between their acceptance and the publication. The journal is indexed in many databases, though not in PubMed. The editorial board should now take steps to index the journal in PubMed. The journal has a system of notifying readers through e-mail when a new issue is released. Also, the articles are available in both the HTML and the PDF formats. I especially like the new and colorful page format of the journal. Also, the access statistics of the articles are available. The prepublication and the manuscript tracking system are also helpful for the authors.
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Overall, the publishing process with JCDR has been smooth, quick and relatively hassle free and I can recommend other authors to consider the journal as an outlet for their work."



Dr. P. Ravi Shankar
KIST Medical College, P.O. Box 14142, Kathmandu, Nepal.
E-mail: ravi.dr.shankar@gmail.com
On April 2011
Anuradha

Dear team JCDR, I would like to thank you for the very professional and polite service provided by everyone at JCDR. While i have been in the field of writing and editing for sometime, this has been my first attempt in publishing a scientific paper.Thank you for hand-holding me through the process.


Dr. Anuradha
E-mail: anuradha2nittur@gmail.com
On Jan 2020

Important Notice

Original article / research
Year : 2022 | Month : May | Volume : 16 | Issue : 5 | Page : SC19 - SC23 Full Version

Diphtheria in Children- Clinical Profile of Cases during an Outbreak in Kerala, India


Published: May 1, 2022 | DOI: https://doi.org/10.7860/JCDR/2022/54907.16376
Thekkile Gangadharan Sindhu, Madhava Vijayakumar, Peethambaran Geetha, Chandran Priya, Puduvail Moorkoth Anitha

1. Additional Professor, Department of Paediatrics, Government Medical College, Kozhikode, Kerala, India. 2. Professor, Department of Paediatrics, Government Medical College, Manjeri, Kerala, India. 3. Additional Professor, Department of Paediatrics, Government Medical College, Kozhikode, Kerala, India. 4. Additional Professor, Department of Community Medicine, Government Medical College, Kozhikode, Kerala, India. 5 Professor, Department of Microbiology, Government Medical College, Manjeri, Kerala, India.

Correspondence Address :
Thekkile Gangadharan Sindhu,
Additional Professor, Department of Paediatrics, Government Medical College, Kozhikode, Kerala, India.
E-mail: drsindhuimch@gmail.com

Abstract

Introduction: Diphtheria is an acute potentially fatal infectious disease caused by the toxigenic strains of Corynebacterium diphtheriae. Acute respiratory obstruction, toxic myocarditis and neurologic weakness are the most important complications of diphtheria. The clinical presentation and severity of diphtheria vary in immunised and non immunised children. Early diagnosis and prompt treatment including administration of diphtheria antitoxin and antibiotics minimise mortality.

Aim: To observe the changing trends in the clinical presentation of diphtheria during the 2016 outbreak and its association with immunisation status and antitoxin administration.

Materials and Methods: This longitudinal prospective study was conducted among children admitted to Government Medical College, Kozhikode, Kerala, a tertiary care centre with a diagnosis of diphtheria during January 2016 to December 2016. Details of socio-demographic data, clinical presentation, investigations, immunisation status, treatment and complications were collected using a semi-structured performa. These children were managed by an interim guideline provided by the state authorities. They were followed-up for 3 months i.e. till March 2017. The data was analysed using Statistical Package for Social Sciences (SPSS), version 18.0.

Results: Among 76 children, 62(81.6%) were from Malappuram and Kozhikode districts, which have relatively low immunisation coverage. Most admissions were in July 2016. Majority 58 (76.3%) of children belonged to Muslim community. The mean age was 8.1 years with male to female ratio 1.53:1. Most of the children 47 (61.8%) were unimmunised or partially immunised. Cultures were positive for C. diphtheriae in 20 children. Complications were noted in 36 children, which included asymptomatic myocarditis in 31, symptomatic myocarditis in one, palatal palsy in nine, loss of accommodation in four and distal weakness in five. Only one child who received antitoxin within 72 hours of disease onset developed neurological complications. Complications were common in children who received less than minimum three doses of diphtheria vaccines compared to those who received three or more doses (54% vs. 44%). There was no mortality.

Conclusion: There was an upward shift in age of affected children. Neurological complications were significantly less in those who received antitoxin within 72 hours of disease onset. Regular monitoring helped to detect asymptomatic myocarditis. The outbreak highlighted the need to improve awareness about diphtheria and better vaccination coverage, especially in older children.

Keywords

Clinical presentation, Complications, Diphtheria antitoxin, Immunization status, Vaccination

Diphtheria is an acute potentially fatal infectious disease caused by the toxigenic strains of Corynebacterium diphtheriae (1). In the pre-vaccine era, more than 40% of cases occurred in children below five years. Recently an upward shift in age is observed both in developed and developing countries (2),(3). Acute respiratory obstruction, toxic myocarditis and neurologic weakness are the most important complications of this disease. Cardiac involvement may be asymptomatic {characterised only by changes in Electrocardiogram (ECG) and/or raised cardiac enzymes) or symptomatic (with clinical features of heart failure)} (4). The main modality of treatment is administration of diphtheria antitoxin and antibiotics. The rapidity of seeking medical care and administering specific treatment is known to decrease the mortality (1). Immunisation status of the individual affects the clinical presentation and severity of the disease (5).

An outbreak of diphtheria was reported from the northern districts of the state of Kerala India in 2016, starting from June 2016. A total of 533 cases were reported accounting for about 7% of globally reported cases (6). But this was based on surveillance data alone and did not analyse the clinical features or treatment response. This was the largest epidemic in Kerala over the last decade. Majority of cases were reported from Kozhikode, Malappuram, and Kannur districts where the routine immunisation coverage was low at that time.

The aim of this study was to find out the changing trends in the clinical presentation and complications of diphtheria in children during the epidemic. The study also had attempted to find out the association of complications seen in the epidemic with immunisation status and the time of antitoxin administration.

Material and Methods

This longitudinal prospective study was conducted in the Paediatric Infectious Disease Unit at Government Medical College, Kozhikode, Kerala, India (tertiary care teaching institute in North Kerala). The study period was from January 2016 to March 2017. All children satisfying the inclusion criteria were included in the study. This study was approved by Institutional Ethical Committee of Government Medical College Kozhikode (Ref No: GMCKKD/RP 2016/ EC/171). Informed consent was obtained from parents.

The operational definition for clinical diphtheria was taken as an illness characterised by laryngitis, pharyngitis or tonsillitis and an adherent membrane on the tonsils, pharynx or nose as per World Health Organisation (WHO) case definition or a child with clinically compatible history with typical complications such as palatal palsy, myocarditis or peripheral neuropathy (7).

Inclusion criteria: All children below 12 years of age who met the operational definition of diphtheria and were admitted to Paediatric Infectious Disease Unit from January 2016 to December 2016 were included in the study. A total of 80 children met the clinical case definition.

Exclusion criteria: Children in whom an alternate diagnosis was made during evaluation were excluded. So, two cases were diagnosed subsequently as infectious mononucleosis, one case as streptococcal pharyngitis, and one turned out to be a case of acute lymphoblastic leukaemia, were excluded from the study.

Procedure

Details of socio-demographic data, clinical presentation, laboratory investigations, immunisation status, treatment and complications were collected using a semi-structured proforma and from the hospital records. Immunsation status was documented after crosschecking with the available records. Those children who had completed recommended age-appropriate doses of diphtheria toxoid as per national immunisation schedule were considered as completely immunised (8). Those who did not receive even a single dose were considered as non immunised and those who had received less than the recommended doses for age were classified as partially immunised.

Clinical examination: Detailed clinical examination was done to determine the degree of involvement and presence of cardiac and neurological complications. A child with clinical diphtheria and ECG showing non specific changes and or raised cardiac enzymes in the absence of clinical features of cardiac failure was classified as having asymptomatic myocarditis. Throat was examined to determine the type and extent of the diphtheritic membrane.

Laboratory test: The laboratory tests includes complete blood counts, Erythrocyte Sedimentation Rate (ESR), hepatic transaminases, renal function tests and urinalysis were done in all children at the time of admission and during clinical deterioration. ECG was taken soon after admission and was repeated on every alternate day during the inpatient stay, at the time of discharge and during follow-up visits. Further investigations including echocardiography and nerve conduction study was done for cases with features of cardiac or neurological involvement. Troponin-I assay was done in 32 children with ECG abnormalities.

Management and follow-up: All cases were managed as per the interim guidelines provided by the Directorate of Health Services, Kerala state (9) at the time of outbreak. The details of treatment with antitoxin (dose, route, and day of administration) and antibiotics were documented. These children were followed-up at 4 and 6 weeks and at 3 months.

Throat swab culture: Throat swabs for Corneybacteria were collected and cultured immediately on blood agar and potassium tellurite agar. Cultures were done soon after admission and repeated on days 15 and 16. The isolates were confirmed from World Health Organisation Vaccine Preventable Disease Surveillance Laboratory at Thiruvananthapuram using Polymerase Chain Reaction (PCR) and standard biochemical tests. Sugar fermentation tests were done using Hiss’s serum sugar media. Other biochemical reactions tested included urease test, nitrate reduction test, catalase and oxidase reaction. The toxigenicity was detected by PCR for tox+ gene (tox A and tox B) and the phenotypic expression was confirmed using modified Elek’s gel precipitation test (10),(11). Real-time PCR (qPCR) assays were done using hydrolysis probes (TaqMan, Applied Biosystems). The qPCR targets for the RNA polymerase β-subunit-encoding gene (rpoB) and the tox A gene were used. Extraction of DNA was performed using Magna pure 24 System (Roche Life Science). Oligonucleotide primers and probe were designed using the software Primer 3 to target rpoB and the tox gene (12). Elek’s test was done with Elek’s test agar base supplemented with calf serum. A sterile filter paper with 10 IU/mL of antitoxin was used. The test organisms were streaked at 10 mm from the disk along with positive and negative controls. The plates were observed after 24 hour incubation at 37°C. Formation of precipitin lines, due to binding of toxins produced by the strains with the antitoxin released from the filter paper, between the disk and the inoculum was taken as positive.

Statistical Analysis

The data was analysed using Statistical Package for Social Sciences (SPSS) version 18.0. Chi-square test was used as test of significance for qualitative variables. A p-value< 0.05 was considered as statistically significant.

Results

Total of 80 children met the clinical case definition. Among these, four children were excluded from the study as they had an alternate diagnosis during the hospital stay. Of these, two cases were diagnosed subsequently as infectious mononucleosis, one case as streptococcal pharyngitis, and one turned out to be a case of acute lymphoblastic leukaemia. The remaining 76 children were followed-up for 3 months and details were analysed. The mean age of the study population was 8.1 years, and the youngest child was 7 months old. There were more boys with a male to female ratio 1.53:1. Most of these children were from Malappuram and Kozhikode districts. The epidemic started in June with the first case admitted on 13th June 2016. Maximum numbers of cases were admitted in July 2016, at the peak of monsoon season in this state. Thereafter cases started declining and reached a plateau by November 2016 (Table/Fig 1).

Among the study subjects, 22 children were unimmunised and 25 partially immunised (Table/Fig 2). None of the older children received Tetanus and adult Diphtheria (Td) vaccination since it was not included in the immunisation schedule at that time.

In this study, 25 (32.9%) cases were diagnosed within 48 hours of the disease onset. One child presented around third week of illness with palatal palsy and another one presented with generalised weakness after one month of illness. All of them had pharyngo-tonsillar lesions (Table/Fig 3). Bull neck was present in 15 (19.7%) cases (Table/Fig 4).

Corynebacterium diphtheriae was isolated from throat swabs of 20 children (26.3%). All isolates were sensitive to penicillin and erythromycin. Repeat cultures after 14 days of treatment were negative except in one child who became culture-negative after a course of erythromycin for 14 days. Culture positive cases included five fully immunised children as well. Thirty four (44.7%) children had leukocyte count above 15000. Erythrocyte Sedimentation Rate (ESR) was above 50 mm in 28 (36.8%) children. Ten (13.2%) of them had elevated Alanine Aminotransferase (ALT) level (Table/Fig 5).

Injection crystalline penicillin was given in a dose of 150000 units /kg/day in four divided doses for 14 days to all subjects (including those who presented late) except one who developed hypersensitivity reaction to penicillin. This child was treated with erythromycin 40 mg/kg/day four divided doses for 14 days. Diphtheria antitoxin was administered to 74 children but could not be given to two since they were admitted two weeks after the onset of symptoms. Antitoxin was given in varying doses (20,000 to 1,00,000 units) depending on the day of presentation and the severity of the illness (Table/Fig 6). For all the children antitoxin was administered intravenously. Less than half 34 (44.7%) of the children received antitoxin within 72 hours of the onset of the disease. Fourteen children had allergic reactions following antitoxin administration. Three of them developed severe allergic reactions and needed desensitisation. Full dose of the antitoxin could not be administered to these three children.

Complications were noted in 36 children while on treatment or follow-up (Table/Fig 7). Palatal palsy was noted in 9 (11.8%) children of whom two developed weakness in the first week itself and 7 after second week. Five children developed distal weakness during 4-6 weeks of illness. One child required ventilator support for 5 days. Four children developed loss of accommodation reflex during follow-up visit but improved without any sequelae. None had any airway compromise. None had jaundice or features of hepatic failure. There was no mortality.

These children were followed-up till 3 months after the onset of illness. New complications noticed during the follow-up visits as well (Table/Fig 8). All affected children recovered completely by three months.

Association between day of administration of antitoxin and complications: Only one child who received antitoxin within 72 hours of disease onset developed neurological complications and this was statistically significant (p-value <0.05).

Association between immunisation status and complications: Complications were common in children who received less than 3 doses of diphtheria toxoid compared to those who received three or more doses (54 vs 44%). However, this was not statistically significant (p-value=0.2). Children who received five doses of vaccine did not develop diphtheritic polyneuropathy or accommodation palsy. Those children with bull neck had developed more complications and it was statistically significant (p-value=0.001).

Discussion

Out of the 533 reported diphtheria cases in Kerala in 2016, 76 cases were admitted in this hospital (Table/Fig 9). This includes 58 children in the under 10 years of age. The upward shift in age in the study by Sangal L et al., is evident in our study as well with relatively lesser number of children in the 0-5 year age group (6). The upward shift of age was reported from various parts of the world (2),(3),(13). Some areas of India continue to have a predilection to younger age groups. In a hospital-based study conducted by Maheriya KM et al., (14) from Ahmadabad (Gujarat), children in the 0-5 year age category were more affected. More boys were affected in current study, which was consistent with the above study. Majority of cases were from Malappuram and Kozhikode districts, which have relatively low immunisation coverage for even primary doses of Diphtheria Pertussis Tetanus (DPT) (80.8% and 86.9%; NFHS 4).

Twenty nine (38.15%) immunised children developed the disease. This is contrary to most of the studies from India where many were unimmunised (Table/Fig 10) (14),(15). But in a recent (2015) epidemic reported from Delhi, a significant number of immunised children developed the disease (16). More than 90% of children developed protective level of antibodies against diphtheria following three primary doses (17). But Gowin E et al., demonstrated that only 70% polish children developed protective antibodies after adequate immunisation pointing to the fact that variation can occur in protective antibody production in different populations (18). So, in the present study, the observation of occurrence of the disease in children adequately immunised points the need for population-based studies for estimating protective antibody levels in India. Recent change in the national policy to give Td instead of Tetanus Toxoid (TT) at 10-year, 15 year and during pregnancy may decrease the disease in these age groups (19). This also reminds us that the protection through immunisation is not an absolute one. Thus, any child presenting with fever and sore throat must be carefully examined for the presence of a diphtheritic membrane irrespective of the immunisation status. It is especially important in an epidemic setting in the background of low immunisation coverage in the community. Bull neck was more common among who had received less than five doses of vaccine. Higher mortality of children with bull neck had been described in an earlier study (16).

Diphtheritic polyneuropathy is under reported in India (20). The latency of the diphtheritic polyneuropathy can vary from 10 days to 3 months (21). In this study, neurological complications appeared within 6 weeks of illness, during follow-up. Lack of follow-up may be the reason for under reporting of neurological complications. Accommodation palsy is not a reported complication in other studies (Table/Fig 10). It was noted that neurological complications were significantly low for those who had received antitoxin early. This observation underscores the importance of early initiation of antitoxin in suspected cases (even before a definite diagnosis is established by bacterial culture).

Cardiac involvement was present in 32 children (42%) of which all except one had asymptomatic myocarditis noted in routine electrocardiogram monitoring. Other studies from India have reported varying incidence of myocarditis from 16 to 66% (4),(5),(14),(22). This highlights the importance of routine ECG monitoring in all children suspected to have diphtheria.

Most of the Indian studies have reported a high case fatality rate varying from 23.67% to 56.3% (5),(14),(15),(16). There was no mortality in current study. The reasons may be due to relatively higher immunisation status, early administration of antitoxin and antibiotics and a protocol based institutional case management for all children with suspected diphtheria.

Limitation(s)

Since this was a hospital-based study, true nature of illness in the community would not be reflected here.

Conclusion

Occurrence of diphtheria in various parts of India including states like Kerala where immunisation coverage in children is significantly high compared to other states, is a matter of grave public health concern. It is important to note that diphtheria may occur in immunised children as well. The clinical profile is also changing with an increased incidence in older children. It is gratifying to note that mortality can be prevented or reduced significantly with institutional care and protocol-based management, which includes routine screening for the development of complications. Administration of diphtheria antitoxin within 72 hours of presentation decreases the development of neurological complications. Regular anticipatory screening for cardiac involvement has a specific role in management.

Acknowledgement

The authors would like to acknowledge Puthezhath S. N Menon Consultant Paediatrician for final preparation of the manuscript. The also would like to acknowledge faculty members, the resident doctors and the nursing staff of the Department of Paediatrics who provided all basic support for the diagnosis and management of patients seen during the outbreak. They would like to acknowledge the help and advice received from the Directorate of Health Services, Kerala.

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DOI and Others

DOI: 10.7860/JCDR/2022/54907.16376

Date of Submission: Jan 12, 2022
Date of Peer Review: Mar 16, 2022
Date of Acceptance: Apr 25, 2022
Date of Publishing: May 01, 2022

AUTHOR DECLARATION:
• Financial or Other Competing Interests: None
• Was Ethics Committee Approval obtained for this study? Yes
• Was informed consent obtained from the subjects involved in the study? Yes
• For any images presented appropriate consent has been obtained from the subjects. No

PLAGIARISM CHECKING METHODS:
• Plagiarism X-checker: Jan 14, 2022
• Manual Googling: Mar 16, 2022
• iThenticate Software: Apr 25, 2022 (4%)

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