Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Dr Mohan Z Mani

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Believers Church Medical College,
Thiruvalla, Kerala
On Sep 2018




Prof. Somashekhar Nimbalkar

"Over the last few years, we have published our research regularly in Journal of Clinical and Diagnostic Research. Having published in more than 20 high impact journals over the last five years including several high impact ones and reviewing articles for even more journals across my fields of interest, we value our published work in JCDR for their high standards in publishing scientific articles. The ease of submission, the rapid reviews in under a month, the high quality of their reviewers and keen attention to the final process of proofs and publication, ensure that there are no mistakes in the final article. We have been asked clarifications on several occasions and have been happy to provide them and it exemplifies the commitment to quality of the team at JCDR."



Prof. Somashekhar Nimbalkar
Head, Department of Pediatrics, Pramukhswami Medical College, Karamsad
Chairman, Research Group, Charutar Arogya Mandal, Karamsad
National Joint Coordinator - Advanced IAP NNF NRP Program
Ex-Member, Governing Body, National Neonatology Forum, New Delhi
Ex-President - National Neonatology Forum Gujarat State Chapter
Department of Pediatrics, Pramukhswami Medical College, Karamsad, Anand, Gujarat.
On Sep 2018




Dr. Kalyani R

"Journal of Clinical and Diagnostic Research is at present a well-known Indian originated scientific journal which started with a humble beginning. I have been associated with this journal since many years. I appreciate the Editor, Dr. Hemant Jain, for his constant effort in bringing up this journal to the present status right from the scratch. The journal is multidisciplinary. It encourages in publishing the scientific articles from postgraduates and also the beginners who start their career. At the same time the journal also caters for the high quality articles from specialty and super-specialty researchers. Hence it provides a platform for the scientist and researchers to publish. The other aspect of it is, the readers get the information regarding the most recent developments in science which can be used for teaching, research, treating patients and to some extent take preventive measures against certain diseases. The journal is contributing immensely to the society at national and international level."



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Professor and Head
Department of Pathology
Sri Devaraj Urs Medical College
Sri Devaraj Urs Academy of Higher Education and Research , Kolar, Karnataka
On Sep 2018




Dr. Saumya Navit

"As a peer-reviewed journal, the Journal of Clinical and Diagnostic Research provides an opportunity to researchers, scientists and budding professionals to explore the developments in the field of medicine and dentistry and their varied specialities, thus extending our view on biological diversities of living species in relation to medicine.
‘Knowledge is treasure of a wise man.’ The free access of this journal provides an immense scope of learning for the both the old and the young in field of medicine and dentistry as well. The multidisciplinary nature of the journal makes it a better platform to absorb all that is being researched and developed. The publication process is systematic and professional. Online submission, publication and peer reviewing makes it a user-friendly journal.
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Dr Saumya Navit
Professor and Head
Department of Pediatric Dentistry
Saraswati Dental College
Lucknow
On Sep 2018




Dr. Arunava Biswas

"My sincere attachment with JCDR as an author as well as reviewer is a learning experience . Their systematic approach in publication of article in various categories is really praiseworthy.
Their prompt and timely response to review's query and the manner in which they have set the reviewing process helps in extracting the best possible scientific writings for publication.
It's a honour and pride to be a part of the JCDR team. My very best wishes to JCDR and hope it will sparkle up above the sky as a high indexed journal in near future."



Dr. Arunava Biswas
MD, DM (Clinical Pharmacology)
Assistant Professor
Department of Pharmacology
Calcutta National Medical College & Hospital , Kolkata




Dr. C.S. Ramesh Babu
" Journal of Clinical and Diagnostic Research (JCDR) is a multi-specialty medical and dental journal publishing high quality research articles in almost all branches of medicine. The quality of printing of figures and tables is excellent and comparable to any International journal. An added advantage is nominal publication charges and monthly issue of the journal and more chances of an article being accepted for publication. Moreover being a multi-specialty journal an article concerning a particular specialty has a wider reach of readers of other related specialties also. As an author and reviewer for several years I find this Journal most suitable and highly recommend this Journal."
Best regards,
C.S. Ramesh Babu,
Associate Professor of Anatomy,
Muzaffarnagar Medical College,
Muzaffarnagar.
On Aug 2018




Dr. Arundhathi. S
"Journal of Clinical and Diagnostic Research (JCDR) is a reputed peer reviewed journal and is constantly involved in publishing high quality research articles related to medicine. Its been a great pleasure to be associated with this esteemed journal as a reviewer and as an author for a couple of years. The editorial board consists of many dedicated and reputed experts as its members and they are doing an appreciable work in guiding budding researchers. JCDR is doing a commendable job in scientific research by promoting excellent quality research & review articles and case reports & series. The reviewers provide appropriate suggestions that improve the quality of articles. I strongly recommend my fraternity to encourage JCDR by contributing their valuable research work in this widely accepted, user friendly journal. I hope my collaboration with JCDR will continue for a long time".



Dr. Arundhathi. S
MBBS, MD (Pathology),
Sanjay Gandhi institute of trauma and orthopedics,
Bengaluru.
On Aug 2018




Dr. Mamta Gupta,
"It gives me great pleasure to be associated with JCDR, since last 2-3 years. Since then I have authored, co-authored and reviewed about 25 articles in JCDR. I thank JCDR for giving me an opportunity to improve my own skills as an author and a reviewer.
It 's a multispecialty journal, publishing high quality articles. It gives a platform to the authors to publish their research work which can be available for everyone across the globe to read. The best thing about JCDR is that the full articles of all medical specialties are available as pdf/html for reading free of cost or without institutional subscription, which is not there for other journals. For those who have problem in writing manuscript or do statistical work, JCDR comes for their rescue.
The journal has a monthly publication and the articles are published quite fast. In time compared to other journals. The on-line first publication is also a great advantage and facility to review one's own articles before going to print. The response to any query and permission if required, is quite fast; this is quite commendable. I have a very good experience about seeking quick permission for quoting a photograph (Fig.) from a JCDR article for my chapter authored in an E book. I never thought it would be so easy. No hassles.
Reviewing articles is no less a pain staking process and requires in depth perception, knowledge about the topic for review. It requires time and concentration, yet I enjoy doing it. The JCDR website especially for the reviewers is quite user friendly. My suggestions for improving the journal is, more strict review process, so that only high quality articles are published. I find a a good number of articles in Obst. Gynae, hence, a new journal for this specialty titled JCDR-OG can be started. May be a bimonthly or quarterly publication to begin with. Only selected articles should find a place in it.
An yearly reward for the best article authored can also incentivize the authors. Though the process of finding the best article will be not be very easy. I do not know how reviewing process can be improved. If an article is being reviewed by two reviewers, then opinion of one can be communicated to the other or the final opinion of the editor can be communicated to the reviewer if requested for. This will help one’s reviewing skills.
My best wishes to Dr. Hemant Jain and all the editorial staff of JCDR for their untiring efforts to bring out this journal. I strongly recommend medical fraternity to publish their valuable research work in this esteemed journal, JCDR".



Dr. Mamta Gupta
Consultant
(Ex HOD Obs &Gynae, Hindu Rao Hospital and associated NDMC Medical College, Delhi)
Aug 2018




Dr. Rajendra Kumar Ghritlaharey

"I wish to thank Dr. Hemant Jain, Editor-in-Chief Journal of Clinical and Diagnostic Research (JCDR), for asking me to write up few words.
Writing is the representation of language in a textual medium i e; into the words and sentences on paper. Quality medical manuscript writing in particular, demands not only a high-quality research, but also requires accurate and concise communication of findings and conclusions, with adherence to particular journal guidelines. In medical field whether working in teaching, private, or in corporate institution, everyone wants to excel in his / her own field and get recognised by making manuscripts publication.


Authors are the souls of any journal, and deserve much respect. To publish a journal manuscripts are needed from authors. Authors have a great responsibility for producing facts of their work in terms of number and results truthfully and an individual honesty is expected from authors in this regards. Both ways its true "No authors-No manuscripts-No journals" and "No journals–No manuscripts–No authors". Reviewing a manuscript is also a very responsible and important task of any peer-reviewed journal and to be taken seriously. It needs knowledge on the subject, sincerity, honesty and determination. Although the process of reviewing a manuscript is a time consuming task butit is expected to give one's best remarks within the time frame of the journal.
Salient features of the JCDR: It is a biomedical, multidisciplinary (including all medical and dental specialities), e-journal, with wide scope and extensive author support. At the same time, a free text of manuscript is available in HTML and PDF format. There is fast growing authorship and readership with JCDR as this can be judged by the number of articles published in it i e; in Feb 2007 of its first issue, it contained 5 articles only, and now in its recent volume published in April 2011, it contained 67 manuscripts. This e-journal is fulfilling the commitments and objectives sincerely, (as stated by Editor-in-chief in his preface to first edition) i e; to encourage physicians through the internet, especially from the developing countries who witness a spectrum of disease and acquire a wealth of knowledge to publish their experiences to benefit the medical community in patients care. I also feel that many of us have work of substance, newer ideas, adequate clinical materials but poor in medical writing and hesitation to submit the work and need help. JCDR provides authors help in this regards.
Timely publication of journal: Publication of manuscripts and bringing out the issue in time is one of the positive aspects of JCDR and is possible with strong support team in terms of peer reviewers, proof reading, language check, computer operators, etc. This is one of the great reasons for authors to submit their work with JCDR. Another best part of JCDR is "Online first Publications" facilities available for the authors. This facility not only provides the prompt publications of the manuscripts but at the same time also early availability of the manuscripts for the readers.
Indexation and online availability: Indexation transforms the journal in some sense from its local ownership to the worldwide professional community and to the public.JCDR is indexed with Embase & EMbiology, Google Scholar, Index Copernicus, Chemical Abstracts Service, Journal seek Database, Indian Science Abstracts, to name few of them. Manuscriptspublished in JCDR are available on major search engines ie; google, yahoo, msn.
In the era of fast growing newer technologies, and in computer and internet friendly environment the manuscripts preparation, submission, review, revision, etc and all can be done and checked with a click from all corer of the world, at any time. Of course there is always a scope for improvement in every field and none is perfect. To progress, one needs to identify the areas of one's weakness and to strengthen them.
It is well said that "happy beginning is half done" and it fits perfectly with JCDR. It has grown considerably and I feel it has already grown up from its infancy to adolescence, achieving the status of standard online e-journal form Indian continent since its inception in Feb 2007. This had been made possible due to the efforts and the hard work put in it. The way the JCDR is improving with every new volume, with good quality original manuscripts, makes it a quality journal for readers. I must thank and congratulate Dr Hemant Jain, Editor-in-Chief JCDR and his team for their sincere efforts, dedication, and determination for making JCDR a fast growing journal.
Every one of us: authors, reviewers, editors, and publisher are responsible for enhancing the stature of the journal. I wish for a great success for JCDR."



Thanking you
With sincere regards
Dr. Rajendra Kumar Ghritlaharey, M.S., M. Ch., FAIS
Associate Professor,
Department of Paediatric Surgery, Gandhi Medical College & Associated
Kamla Nehru & Hamidia Hospitals Bhopal, Madhya Pradesh 462 001 (India)
E-mail: drrajendrak1@rediffmail.com
On May 11,2011




Dr. Shankar P.R.

"On looking back through my Gmail archives after being requested by the journal to write a short editorial about my experiences of publishing with the Journal of Clinical and Diagnostic Research (JCDR), I came across an e-mail from Dr. Hemant Jain, Editor, in March 2007, which introduced the new electronic journal. The main features of the journal which were outlined in the e-mail were extensive author support, cash rewards, the peer review process, and other salient features of the journal.
Over a span of over four years, we (I and my colleagues) have published around 25 articles in the journal. In this editorial, I plan to briefly discuss my experiences of publishing with JCDR and the strengths of the journal and to finally address the areas for improvement.
My experiences of publishing with JCDR: Overall, my experiences of publishing withJCDR have been positive. The best point about the journal is that it responds to queries from the author. This may seem to be simple and not too much to ask for, but unfortunately, many journals in the subcontinent and from many developing countries do not respond or they respond with a long delay to the queries from the authors 1. The reasons could be many, including lack of optimal secretarial and other support. Another problem with many journals is the slowness of the review process. Editorial processing and peer review can take anywhere between a year to two years with some journals. Also, some journals do not keep the contributors informed about the progress of the review process. Due to the long review process, the articles can lose their relevance and topicality. A major benefit with JCDR is the timeliness and promptness of its response. In Dr Jain's e-mail which was sent to me in 2007, before the introduction of the Pre-publishing system, he had stated that he had received my submission and that he would get back to me within seven days and he did!
Most of the manuscripts are published within 3 to 4 months of their submission if they are found to be suitable after the review process. JCDR is published bimonthly and the accepted articles were usually published in the next issue. Recently, due to the increased volume of the submissions, the review process has become slower and it ?? Section can take from 4 to 6 months for the articles to be reviewed. The journal has an extensive author support system and it has recently introduced a paid expedited review process. The journal also mentions the average time for processing the manuscript under different submission systems - regular submission and expedited review.
Strengths of the journal: The journal has an online first facility in which the accepted manuscripts may be published on the website before being included in a regular issue of the journal. This cuts down the time between their acceptance and the publication. The journal is indexed in many databases, though not in PubMed. The editorial board should now take steps to index the journal in PubMed. The journal has a system of notifying readers through e-mail when a new issue is released. Also, the articles are available in both the HTML and the PDF formats. I especially like the new and colorful page format of the journal. Also, the access statistics of the articles are available. The prepublication and the manuscript tracking system are also helpful for the authors.
Areas for improvement: In certain cases, I felt that the peer review process of the manuscripts was not up to international standards and that it should be strengthened. Also, the number of manuscripts in an issue is high and it may be difficult for readers to go through all of them. The journal can consider tightening of the peer review process and increasing the quality standards for the acceptance of the manuscripts. I faced occasional problems with the online manuscript submission (Pre-publishing) system, which have to be addressed.
Overall, the publishing process with JCDR has been smooth, quick and relatively hassle free and I can recommend other authors to consider the journal as an outlet for their work."



Dr. P. Ravi Shankar
KIST Medical College, P.O. Box 14142, Kathmandu, Nepal.
E-mail: ravi.dr.shankar@gmail.com
On April 2011
Anuradha

Dear team JCDR, I would like to thank you for the very professional and polite service provided by everyone at JCDR. While i have been in the field of writing and editing for sometime, this has been my first attempt in publishing a scientific paper.Thank you for hand-holding me through the process.


Dr. Anuradha
E-mail: anuradha2nittur@gmail.com
On Jan 2020

Important Notice

Original article / research
Year : 2022 | Month : September | Volume : 16 | Issue : 9 | Page : ZC24 - ZC29 Full Version

Efficacy of Rotary Retreatment Techniques Assisted with Passive Ultrasonic Activation of Resin Solvent in Removal of Gutta-percha with Epoxy Resin and MTA Based Root Canal Sealers: An In-vitro Study


Published: September 1, 2022 | DOI: https://doi.org/10.7860/JCDR/2022/55339.16956
Akash Kiran More, MV Sumanthini, Vanitha U Shenoy

1. Resident, Department of Conservative Dentistry and Endodontics, Mahatma Gandhi’s Mission Dental College and Hospital, Navi Mumbai, Maharashtra, India. 2. Professor and Head, Department of Conservative Dentistry and Endodontics, Mahatma Gandhi’s Mission Dental College and Hospital, Navi Mumbai, Maharashtra, India. 3. Ex-Professor and Head, Department of Conservative Dentistry and Endodontics, Mahatma Gandhi’s Mission Dental College and Hospital, Navi Mumbai, Maharashtra, India.

Correspondence Address :
Dr. Akash Kiran More,
201, Sairaj Sadan, Plot No 36, Sanpada, Navi Mumbai, Maharashtra, India.
E-mail: akmore33@gmail.com

Abstract

Introduction: Endodontic retreatment is indicated in cases of failed primary endodontic treatment. The main goal of retreatment is to establish conditions that will enable the healing of the periapical tissue.

Aim: To evaluate the efficacy of rotary retreatment with the ProTaper Universal Retreatment system supplemented with passive ultrasonic activation of solvent in retreatment of single-rooted mandibular premolar teeth obturated with gutta-percha and two different root canal sealers (AH Plus and MTA Fillapex).

Materials and Methods: This in-vitro study was conducted in the Department of Conservative Dentistry and Endodontics at Mahatma Gandhi Mission’s Dental College and Hospital, Navi Mumbai, Maharashtra, India, from January 2019 to April 2019. Total 66 freshly extracted human single rooted mandibular premolars were used in this study. Root canal preparation was done using ProTaper Next rotary files. Teeth were decoronated to get a uniform length of 18 mm and were divided into three retreatment groups based on the technique used for retreatment. Group I included rotary retreatment, Group II involved rotary retreatment with solvent and paper point wicking, and Group III involved rotary retreatment with passive ultrasonic activation of solvent and paper point wicking. Based on the sealer used for obturation, teeth in each group were further divided into two subgroups, subgroup A with AH Plus sealer and subgroup B with MTA Fillapex sealer. Teeth were stored in an incubator for 30 days to allow sealer to set after which sectioning of the specimens was done buccolingually for examination under the stereomicroscope and photographed. Statistical analysis was done by using parametric Analysis of Variance (ANOVA) followed by Post-hoc tests and Unpaired t-test.

Results: No statistically significant difference in percentage area of residual root canal filling material noted between the group I and II and when obturated with gutta-percha and AH Plus sealer (subgroup A) as p-value=0.215. Whereas, it revealed a statistically significant difference between the group I, II, and III when obturated with gutta-percha and MTA Fillapex sealer (subgroup B) as p-value<0.001.

Conclusion: Passive ultrasonic activation of solvent after gutta-percha removal using ProTaper Universal rotary files could improve the quality of endodontic retreatment.

Keywords

AH Plus sealer, Endosolv R, MTA Fillapex root canal sealer, ProTaper universal retreatment file, Stereomicroscope

A successful outcome of the retreatment procedure depends on the effective removal of filling material from the root canal system (1). An effectively performed procedure promotes better cleaning and disinfection because instruments and irrigating solutions reach the entire root canal system (2). There are several techniques for removing Gutta-percha (GP) and sealer from filled root canals including hand files, burs, automated devices, rotary, reciprocating instruments, and sonic or ultrasonic irrigation which are generally preceded by softening of the filling material with different solvents or heat (1). Different rotary instrumentation systems have been developed specifically such as XP-endo finisher, RaCe, FlexMaster, and ProTaper Universal Retreatment (PTUR) system for removal of gutta-percha and sealer. However, a small amount of residual gutta-percha and sealer was observed to be left on canal walls post retreatment as observed in stereomicroscopic studies (3). All retreatment techniques leave residual debris in the canal walls after reinstrumentation (4). Necrotic tissue or bacteria, covered by the remaining GP or sealer may be responsible for periapical inflammation or pain and complete removal of it is necessary for a successful outcome. This enables thorough chemomechanical reinstrumentation and disinfection of the root canal system (3). The primary goal of root canal retreatment is to stop the infectious process through the removal of filling material, debris, and microorganisms that cause apical periodontitis (5). To remove residual obturating material, passive ultrasonic activation has been suggested as a supplemental technique in addition to the routine retreatment methods. During root canal retreatment, a solvent can be used to facilitate the removal of gutta-percha by softening it. Gutta-percha and most sealers are miscible in chloroform, xylene, and endosolv and once in the solution can be absorbed and removed with appropriately sized absorbent points (6). Passive Ultrasonic Activation (PUA) involves ultrasonically activating a file inside a root canal filled with an endodontic irrigant (7).

Isthmus and accessory canals filled with gutta-percha and sealer are very difficult to remove during retreatment as these endodontic sealers penetrate deep in dentinal tubules leaving back residual filling materials (8). To date, few reports have studied the effectiveness of rotary systems supplemented with passive ultrasonic activation of solvent while removing root canal filling material (8),(9),(10). None of the studies have evaluated the effect of PUA in the removal of MTA Fillapex root canal sealer.

The null hypothesis states that there will be no improvement in the efficacy of rotary retreatment when supplemented with passive ultrasonic activation of solvent in the removal of gutta-percha in epoxy resin-based sealer or gutta-percha in MTA based sealer from the root canal treated teeth. The aim of this study was to evaluate whether passive ultrasonic activation of solvent will enhance the removal of gutta-percha and root canal sealer.

Material and Methods

This in-vitro study was conducted in the Department of Conservative Dentistry and Endodontics at Mahatma Gandhi Mission’s Dental College and Hospital, Navi Mumbai, Maharashtra, India, from January 2019 to April 2019. Ethical approval obtained from Institutional Ethics Committee (MGM/DCH/IERC/492/17). A total of 66 freshly extracted human single rooted mandibular premolar teeth were chosen for the study.

Inclusion criteria: Permanent human single rooted mandibular premolars with a single root canal extracted for periodontal and orthodontic reasons, intact teeth with no cracks or defects, teeth with similar crown-root dimensions, without caries, having a single root canal with closed or mature apices, with a patent root canal and teeth with a straight canal with minimal curvature (0 to 10 degrees) were included in the study.

Exclusion criteria: Deciduous teeth, permanent human teeth with multiple roots and canals, teeth with pre-existing dentinal cracks or microcracks or fracture defects, teeth with caries, hypoplastic defects, external and internal root resorption, restorations, anatomic malformations, root canals with calcifications and the root canal treated teeth were excluded from the study.

Endodontic Treatment

The selected teeth were immersed in 5% sodium hypochlorite (NaOCl, Trifarma, India) for an hour to remove any organic debris. All 66 teeth were decoronated with a diamond disc to get a uniform length of 18 mm. All the root canals were instrumented by the same operator using ProTaper Next (PTN) (Dentsply, Tulsa Dental, Switzerland) rotary system. Canal preparation was done using a crown down approach till instrument size X3 (#30, 0.07) followed by circumferential filing with the Master Apical File (MAF). Throughout instrumentation, 25 mL 5% sodium hypochlorite (Trifarma, India) was delivered from a 30-gauge irrigation side venting needle (Neo Endo, London United Kingdom) between each file. Once instrumentation was completed, Passive Ultrasonic Irrigation (PUI) was performed in all the teeth using a No. 25 Irrisafe ultrasonic tip (Acteon, Satelec United Kingdom) kept 2 mm short of working length at a power setting of 3 with 5% sodium hypochlorite solution for three cycles of 20 seconds for a total volume of 6 mL. This was followed by 17% Ethylenediamine Tetra Acetic acid (EDTA, META BIOMED, Korea) and similarly for three cycles of 20 seconds for a total volume of 6 mL of 17% EDTA. This was followed by flushing the canals with 6 mL of 5% sodium hypochlorite and would be finalized with irrigation using 20 mL distilled water (Nir life, India).

The selected teeth were randomly assigned into three groups based on the retreatment technique to be carried out with 22 teeth in each group. Each group was divided into two subgroups based on the sealer used along with gutta-percha for obturation. Teeth belonging to subgroup A were obturated with GP and epoxy resin-based sealer (AH Plus) (Dentsply, Tulsa Dental, Switzerland) while those in subgroup B were obturated with GP and mineral trioxide aggregate (MTA) based sealer (MTA Fillapex) (Angelus, Londrina, Parana, Brazil) (Table/Fig 1). The sealers were manipulated as per the manufacturer’s instructions.

The quality of root filling was deemed adequate when no voids were seen on radiographs and obturation appeared dense (11). Access opening was temporized with Intermediate Restorative Material (IRM) (Dentsply, Tulsa Dental, Switzerland). The specimens were stored under 100% humidity at 37°C for 30 days to allow the sealer to set completely in an incubator (Dolphin, India) (12).

Rotary retreatment was carried out using ProTaper Universal Rotary Retreatment system (PTUR, Dentsply, Tulsa Dental, Switzerland). Endosolv R was used as a solvent during the retreatment procedure. #25 Irrisafe ultrasonic tip (Irrisafe tips, Acteon, Merignac, France) was used for Passive Ultrasonic Activation of solvent.

Endodontic Retreatment

Group I: Gates Glidden drill (Mani Inc, Japan) sizes 2 and 3 were used in the first 3 mm of the root canal coronally. Root fillings were removed with PTUR instruments (Dentsply, Tulsa Dental, Switzerland) following the manufacturer’s instructions. Rotary retreatment files D1, D2, and D3 were sequentially used in a crown-down manner at a speed of 500 rpm and torque of 3N/Cm to reach the pre-established working length; they were manipulated in a brushing action. A single operator performed all filling removal protocols. The rotary instruments were used for five teeth and then discarded.

Group II: Rotary retreatment was supplemented with Endosolv R solvent and the procedure was carried out similar to the technique described in group I. A 0.1 mL of Endosolv R was introduced in the root canal to soften the gutta-percha for 30 seconds before instrumentation. The solvent-filled canal was then dried with absorbent points called ‘wicking’; this is the final stage in GP removal (12). The canal was flooded with 1 mL of Endosolv R and the solution was absorbed and removed with appropriately sized absorbent points (12). The canal was replenished with 1 mL of solvent and the above procedure was repeated till the absorbent points came out dry. Absorbent points aided in the removal of GP by drawing dissolved materials into and then out of the shaped canal. Even when absorbent points came out of the canal clean, white, and dry, it was assumed that residual GP and sealer might be still present (13).

Group III: Rotary retreatment was done according to the technique described in group I. The canal was flooded with 1 mL of Endosolv R and passive ultrasonic activation of it was performed for one minute in three cycles of 20 seconds, with a No 25 Irri safe ultrasonic tip at 1 mm short of the working length, in an up-and-down motion. The ultrasonic handpiece (Satelac, Acteon, Merignac, France) was set to operate at a power setting of 3. The solvent solution was replenished after each cycle of 20 seconds. The solution was then absorbed and removed with appropriately sized absorbent points. Paper point wicking was done according to the technique described in group II.

Irrigation protocol during retreatment: Irrigation during filling removal was performed using a total of 25 mL of 5% NaOCl solution per tooth in each group. The smear layer in each tooth was removed by irrigation with 5 mL 17% EDTA for 3 minutes (14). This was followed by flushing the canals with 5 mL of 5% sodium hypochlorite and was finally irrigated using 5 mL distilled water. Distilled water acts as an inert solution that does not possess damaging properties to dentinal structure (15). The filling removal procedure was considered complete when no further filling material was evident adhering to the instrument used during retreatment (16).

Coding of specimens and assessment of obturation removal: Coding of the specimens was done by an independent observer to remove operator bias before the evaluation of residual filling material. To evaluate the residual filling material along the root canal wall, the teeth were grooved buccolingually using a high-speed diamond disk and sectioned longitudinally using a chisel. Digital images were obtained using the stereomicroscope (Moticam, Hongkong) and Motic camera (Moticam, Hongkong) by placing the sectioned tooth on a black platform under 1X magnification and were transferred to a computer. The photographs of the specimens obtained were captured as jpeg images (Table/Fig 2)a-f. The residual filling material was assessed by loading the images into motic imaging software (Moticam, Hongkong) and measuring the area of the remaining filling material (by tracing the residual filling material inside the root canal) relative to the total root canal area (by tracing the root canal outline) (9) (Table/Fig 3), (Table/Fig 4). Three readings were taken and amongst them, the average value was considered. The percentage of the remaining filling material on the canal walls was calculated with the following equation (9).

% Area of remaining filling material= Area of remaining filling material / Area of canal space × 100

Statistical Analysis

Statistical analysis was done using Statistical Package for Social Sciences software (IBM SPSS Statistics, University of Chicago) version 16.0. The data obtained were presented using descriptive statistics such as mean, standard deviation, maximum and minimum values The mean percentage values relative to the total canal area were compared. Normality of the data was tested using Kolmogorov-Smirnov test. The data was found to be normally distributed and further analysis was done using parametric Analysis of Variance (ANOVA) followed by Post-hoc tests and Unpaired t-test. The level of significance was set at 5%. All p-values < 0.05 were treated as significant.

Results

Mean, median, minimum and maximum values of the percentage area of remaining filling material for groups I, II, and III are shown in (Table/Fig 5). For teeth obturated with AH Plus sealer, the mean percentage area of residual filling material ranged from 27.15 to 34.21% with the maximum amount of residual filling material seen in group I (rotary retreatment) and minimum amount seen with group III {(rotary retreatment with Passive Ultrasonic Activation (PUA) of solvent)} with a Standard Deviation (SD) of 6.94 to 10.42. For teeth obturated with MTA Fillapex sealer, the mean percentage area of residual filling material ranged from 13.33 to 29.73% with the maximum amount of residual filling material seen in group II (Rotary Retreatment with solvent) and minimum amount seen with group III (rotary retreatment with passive ultrasonic activation (PUA) of solvent) with a SD of 6.13 to 13.60 and a confidence interval of 95%

The results of one-way ANOVA for the percentage area of canal covered with residual filling material revealed no statistically significant difference between the groups I, II, and III when root canal space was obturated with gutta-percha and AH Plus sealer (subgroup A) as p-value>0.05 which is presented in (Table/Fig 6).

The results of one-way ANOVA for the percentage area of canal covered with residual filling material revealed a statistically significant (p-value<0.001) difference between the groups I, II, and III when root canal space was obturated with gutta-percha and MTA Fillapex sealer (subgroup B) which is presented in (Table/Fig 7).

Post-hoc LSD test comparison revealed there was a statistically significant difference between the three techniques used for the removal of obturating material. The percentage area of residual filling material of teeth where group I (rotary retreatment) was used was statistically significant (p-value<0.05) (Table/Fig 8) when compared with group II (rotary retreatment with solvent) and group III (rotary retreatment with passive ultrasonic activation of solvent). Group II (rotary retreatment with solvent) showed a statistically significant (p-value<0.001) difference (Table/Fig 9) when compared with group III-(rotary retreatment with passive ultrasonic activation of solvent).

Intergroup comparison for differences in the mean percentage area values for groups I, II, and III obturated with AH Plus and MTA Fillapex sealer was done using Unpaired t-test as depicted in (Table/Fig 10). The results of the unpaired t-test analysis revealed that there was no significant difference in the percentage of residual filling material for group I when both AH Plus and MTA Fillapex subgroups were compared. However, there was a significant difference (p-value<0.05) in the percentage of residual filling material for group II and group III when both AH Plus and MTA Fillapex subgroups were compared with t-values.

The percentage area of residual root canal filling material was highest in the coronal and middle 1/3rd of the root for groups I, II, and III. Rotary Retreatment with passive ultrasonic activation of solvent showed the best result in the removal of root canal filling material when compared with other retreatment groups.

Discussion

Inadequate cleaning, shaping, obturation, and final restoration of an endodontically diseased tooth can lead to posttreatment disease (17). If initial endodontic therapy does not render the canal space free of bacteria, if the obturation does not adequately entomb those that may remain, or if new microorganisms are allowed to reenter the cleaned and sealed canal space, the posttreatment disease can and usually does occur (8).

ProTaper Universal Retreatment system which has three retreatment instruments was used in all three groups. D1 (size 30, 0.09 taper), D2 (size 25, 0.08 taper), and D3 (20, 0.07 taper) retreatment series have a convex cross-section with a working tip that facilitates its penetration into filling materials (18). Further root canal refining is necessary because the apical diameter of the D3 PTUR file does not permit a complete cleaning action. The final shaping of the canal was done using X3 ProTaper Next rotary file.

ProTaper Universal Retreatment files showing good results in the removal of root canal filling material in the apical thirds could be explained by the fact that D3 has a tip size of 20 and 7% taper which is closer to the master apical file size of X3 tip (Size 30 with 7% taper) (18). Rotary retreatment was followed by shaping the root canal with the ProTaper Next X3 file. (Table/Fig 11) is showing the comparison of the results of the present study and relevant studies conducted in the past (7),(8),(9),(10),(19),(20),(21),(22).

Organic solvents are a chemical class of compounds that are applied during retreatment to decrease the resistance of filling materials in the root canal, thus facilitating their removal without damaging the tooth (7). Diverse chemical solvents are available, and they dissolve root canal sealers at different intensities. Endosolv R is an organic solvent that contains formamide 50 grams and phenyl ethylene alcohol 50 grams (20). It has been shown to aid in fresh AH Plus removal after filling and to dissolve set AH Plus in vitro.

It has been shown that ultrasonically driven files are effective for the “irrigation” of root canals. Therefore, the use of ultrasonics in canals has evolved from primary instrumentation to a passive cleaning technique (23). Small, intense, circular fluid movement (i.e., eddy flow) around the instrument is created due to acoustic streaming. The maximum eddying effect occurs at the tip of the file compared to the coronal end, with an apically directed flow. An enlarged root canal helps the file or wire to vibrate freely in a way to enables acoustic streaming with the transfer of energy to the irrigant inside the canal (23).

To evaluate the residual filling material both root halves were photographed under a stereomicroscope. Stereomicroscopes are used to study three-dimensional objects, examine small objects, or dissect biological specimens (24). They provide magnification up to 5X which can be used to differentiate between sealer and GP left inside the canal. It provides an attachment for a digital camera which is useful to capture images of the sectioned teeth at different magnification values. Images from a stereomicroscope can be easily transferred to a computer containing imaging software where images are analysed. In the present study percentage area of the residual root canal, filling material was assessed using image analysis software by calculating the total area of the canal in (mm2) and the area covered by residual root canal filling material (mm2).

The results of the present study revealed that rotary retreatment supplemented with PUA passive ultrasonic activation of GP solvent showed the better removal of root canal filling material when compared with other retreatment groups and was statistically significant. Hence, the null hypothesis stands rejected. Lesser residual filling material was observed in teeth with rotary retreatment followed by PUA of solvent in both MTA Fillapex and AH Plus groups. Ultrasonic agitation of organic solvents during retreatment can help to improve the chemical characteristics of these substances by boosting their dissolving capabilities for root canal sealers. The prospect of ultrasound stimulating solvent extrusion and generating adverse effects on periapical tissues calls into question the connection of ultrasound with a solvent (25).

In the present study, the retreatment for MTA Fillapex showed the least amount of residual filling material as compared with AH Plus. This may be attributed to two reasons: the low bond strength of MTA Fillapex to root dentin and the questionable biomineralization of MTA Fillapex (26). The low bond strength of MTA Fillapex confirms the results of recent studies that reported a low adhesion capacity of MTA Fillapex (27),(28),(29). The superior performance widely reported by the literature for the use of MTA was attributed to its biomineralization ability. The interaction of MTA with a phosphate-containing fluid produces calcium-deficient B-type carbonated apatite via an amorphous calcium phosphate phase (26). However, the low bond strength of MTA Fillapex has been attributed to the low adhesion capacity of these tag-like appetites. However, this supposition is in contrast to the findings of Salles LP et al, who noted that MTA Fillapex showed increased alkaline phosphatase activity after seven days, thereby stimulating hydroxyapatite crystal nucleation. Nevertheless, a comparison of MTA Fillapex with other commercial brands of MTA is yet to be performed (30).

The efficacy of a solvent Endosolv R in dissolving a solute or softening a polymer may be explained by the concept “like dissolves like” (i.e., polar solvents are better at dissolving polar compounds) (10). Nonpolar solvents typically will not dissolve polar substances and vice versa. Epoxy resin is probably miscible with the formamide/2-phenyl ethanol cosolvents, enabling it to be dissolved by the Endosolv. Because formamide and 2-phenyl ethanol are miscible with water, this permits the dissolved epoxy resin to be rinsed away by water (20).

Limitation(s)

The limitations of this study included the in-vitro study design which makes it difficult to be compared with clinical situations and the complexity associated with it. It also lacked chemical analysis of the solvent. This would be to quantitatively verify the saturation of the solutions and the interaction pattern of root canal sealers with solvents during PUA.

Conclusion

Within the limitations of the present study, it was observed that passive ultrasonic activation of solvent enhanced the removal of gutta-percha and root canal sealer from root canal-treated teeth more effectively. The retreatment for MTA Fillapex showed the least amount of residual filling material as compared with AH Plus.

However passive ultrasonic activation of solvent seems to be a promising approach for clinicians during retreatment procedures. additional testing with alternative solvents is encouraged. Moreover, a statistically significant finding in this type of in-vitro study does not necessarily imply clinical significance.

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DOI and Others

DOI: 10.7860/JCDR/2022/55339.16956

Date of Submission: Feb 13, 2022
Date of Peer Review: May 06, 2022
Date of Acceptance: Jul 06, 2022
Date of Publishing: Sep 01, 2022

AUTHOR DECLARATION:
• Financial or Other Competing Interests: None
• Was Ethics Committee Approval obtained for this study? Yes
• Was informed consent obtained from the subjects involved in the study? No
• For any images presented appropriate consent has been obtained from the subjects. No

PLAGIARISM CHECKING METHODS:
• Plagiarism X-checker: Feb 17, 2022
• Manual Googling: May 05, 2022
• iThenticate Software: Aug 26, 2022 (25%)

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