Journal of Clinical and Diagnostic Research, ISSN - 0973 - 709X

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Dr Mohan Z Mani

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On Sep 2018




Prof. Somashekhar Nimbalkar

"Over the last few years, we have published our research regularly in Journal of Clinical and Diagnostic Research. Having published in more than 20 high impact journals over the last five years including several high impact ones and reviewing articles for even more journals across my fields of interest, we value our published work in JCDR for their high standards in publishing scientific articles. The ease of submission, the rapid reviews in under a month, the high quality of their reviewers and keen attention to the final process of proofs and publication, ensure that there are no mistakes in the final article. We have been asked clarifications on several occasions and have been happy to provide them and it exemplifies the commitment to quality of the team at JCDR."



Prof. Somashekhar Nimbalkar
Head, Department of Pediatrics, Pramukhswami Medical College, Karamsad
Chairman, Research Group, Charutar Arogya Mandal, Karamsad
National Joint Coordinator - Advanced IAP NNF NRP Program
Ex-Member, Governing Body, National Neonatology Forum, New Delhi
Ex-President - National Neonatology Forum Gujarat State Chapter
Department of Pediatrics, Pramukhswami Medical College, Karamsad, Anand, Gujarat.
On Sep 2018




Dr. Kalyani R

"Journal of Clinical and Diagnostic Research is at present a well-known Indian originated scientific journal which started with a humble beginning. I have been associated with this journal since many years. I appreciate the Editor, Dr. Hemant Jain, for his constant effort in bringing up this journal to the present status right from the scratch. The journal is multidisciplinary. It encourages in publishing the scientific articles from postgraduates and also the beginners who start their career. At the same time the journal also caters for the high quality articles from specialty and super-specialty researchers. Hence it provides a platform for the scientist and researchers to publish. The other aspect of it is, the readers get the information regarding the most recent developments in science which can be used for teaching, research, treating patients and to some extent take preventive measures against certain diseases. The journal is contributing immensely to the society at national and international level."



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Sri Devaraj Urs Medical College
Sri Devaraj Urs Academy of Higher Education and Research , Kolar, Karnataka
On Sep 2018




Dr. Saumya Navit

"As a peer-reviewed journal, the Journal of Clinical and Diagnostic Research provides an opportunity to researchers, scientists and budding professionals to explore the developments in the field of medicine and dentistry and their varied specialities, thus extending our view on biological diversities of living species in relation to medicine.
‘Knowledge is treasure of a wise man.’ The free access of this journal provides an immense scope of learning for the both the old and the young in field of medicine and dentistry as well. The multidisciplinary nature of the journal makes it a better platform to absorb all that is being researched and developed. The publication process is systematic and professional. Online submission, publication and peer reviewing makes it a user-friendly journal.
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Dr Saumya Navit
Professor and Head
Department of Pediatric Dentistry
Saraswati Dental College
Lucknow
On Sep 2018




Dr. Arunava Biswas

"My sincere attachment with JCDR as an author as well as reviewer is a learning experience . Their systematic approach in publication of article in various categories is really praiseworthy.
Their prompt and timely response to review's query and the manner in which they have set the reviewing process helps in extracting the best possible scientific writings for publication.
It's a honour and pride to be a part of the JCDR team. My very best wishes to JCDR and hope it will sparkle up above the sky as a high indexed journal in near future."



Dr. Arunava Biswas
MD, DM (Clinical Pharmacology)
Assistant Professor
Department of Pharmacology
Calcutta National Medical College & Hospital , Kolkata




Dr. C.S. Ramesh Babu
" Journal of Clinical and Diagnostic Research (JCDR) is a multi-specialty medical and dental journal publishing high quality research articles in almost all branches of medicine. The quality of printing of figures and tables is excellent and comparable to any International journal. An added advantage is nominal publication charges and monthly issue of the journal and more chances of an article being accepted for publication. Moreover being a multi-specialty journal an article concerning a particular specialty has a wider reach of readers of other related specialties also. As an author and reviewer for several years I find this Journal most suitable and highly recommend this Journal."
Best regards,
C.S. Ramesh Babu,
Associate Professor of Anatomy,
Muzaffarnagar Medical College,
Muzaffarnagar.
On Aug 2018




Dr. Arundhathi. S
"Journal of Clinical and Diagnostic Research (JCDR) is a reputed peer reviewed journal and is constantly involved in publishing high quality research articles related to medicine. Its been a great pleasure to be associated with this esteemed journal as a reviewer and as an author for a couple of years. The editorial board consists of many dedicated and reputed experts as its members and they are doing an appreciable work in guiding budding researchers. JCDR is doing a commendable job in scientific research by promoting excellent quality research & review articles and case reports & series. The reviewers provide appropriate suggestions that improve the quality of articles. I strongly recommend my fraternity to encourage JCDR by contributing their valuable research work in this widely accepted, user friendly journal. I hope my collaboration with JCDR will continue for a long time".



Dr. Arundhathi. S
MBBS, MD (Pathology),
Sanjay Gandhi institute of trauma and orthopedics,
Bengaluru.
On Aug 2018




Dr. Mamta Gupta,
"It gives me great pleasure to be associated with JCDR, since last 2-3 years. Since then I have authored, co-authored and reviewed about 25 articles in JCDR. I thank JCDR for giving me an opportunity to improve my own skills as an author and a reviewer.
It 's a multispecialty journal, publishing high quality articles. It gives a platform to the authors to publish their research work which can be available for everyone across the globe to read. The best thing about JCDR is that the full articles of all medical specialties are available as pdf/html for reading free of cost or without institutional subscription, which is not there for other journals. For those who have problem in writing manuscript or do statistical work, JCDR comes for their rescue.
The journal has a monthly publication and the articles are published quite fast. In time compared to other journals. The on-line first publication is also a great advantage and facility to review one's own articles before going to print. The response to any query and permission if required, is quite fast; this is quite commendable. I have a very good experience about seeking quick permission for quoting a photograph (Fig.) from a JCDR article for my chapter authored in an E book. I never thought it would be so easy. No hassles.
Reviewing articles is no less a pain staking process and requires in depth perception, knowledge about the topic for review. It requires time and concentration, yet I enjoy doing it. The JCDR website especially for the reviewers is quite user friendly. My suggestions for improving the journal is, more strict review process, so that only high quality articles are published. I find a a good number of articles in Obst. Gynae, hence, a new journal for this specialty titled JCDR-OG can be started. May be a bimonthly or quarterly publication to begin with. Only selected articles should find a place in it.
An yearly reward for the best article authored can also incentivize the authors. Though the process of finding the best article will be not be very easy. I do not know how reviewing process can be improved. If an article is being reviewed by two reviewers, then opinion of one can be communicated to the other or the final opinion of the editor can be communicated to the reviewer if requested for. This will help one’s reviewing skills.
My best wishes to Dr. Hemant Jain and all the editorial staff of JCDR for their untiring efforts to bring out this journal. I strongly recommend medical fraternity to publish their valuable research work in this esteemed journal, JCDR".



Dr. Mamta Gupta
Consultant
(Ex HOD Obs &Gynae, Hindu Rao Hospital and associated NDMC Medical College, Delhi)
Aug 2018




Dr. Rajendra Kumar Ghritlaharey

"I wish to thank Dr. Hemant Jain, Editor-in-Chief Journal of Clinical and Diagnostic Research (JCDR), for asking me to write up few words.
Writing is the representation of language in a textual medium i e; into the words and sentences on paper. Quality medical manuscript writing in particular, demands not only a high-quality research, but also requires accurate and concise communication of findings and conclusions, with adherence to particular journal guidelines. In medical field whether working in teaching, private, or in corporate institution, everyone wants to excel in his / her own field and get recognised by making manuscripts publication.


Authors are the souls of any journal, and deserve much respect. To publish a journal manuscripts are needed from authors. Authors have a great responsibility for producing facts of their work in terms of number and results truthfully and an individual honesty is expected from authors in this regards. Both ways its true "No authors-No manuscripts-No journals" and "No journals–No manuscripts–No authors". Reviewing a manuscript is also a very responsible and important task of any peer-reviewed journal and to be taken seriously. It needs knowledge on the subject, sincerity, honesty and determination. Although the process of reviewing a manuscript is a time consuming task butit is expected to give one's best remarks within the time frame of the journal.
Salient features of the JCDR: It is a biomedical, multidisciplinary (including all medical and dental specialities), e-journal, with wide scope and extensive author support. At the same time, a free text of manuscript is available in HTML and PDF format. There is fast growing authorship and readership with JCDR as this can be judged by the number of articles published in it i e; in Feb 2007 of its first issue, it contained 5 articles only, and now in its recent volume published in April 2011, it contained 67 manuscripts. This e-journal is fulfilling the commitments and objectives sincerely, (as stated by Editor-in-chief in his preface to first edition) i e; to encourage physicians through the internet, especially from the developing countries who witness a spectrum of disease and acquire a wealth of knowledge to publish their experiences to benefit the medical community in patients care. I also feel that many of us have work of substance, newer ideas, adequate clinical materials but poor in medical writing and hesitation to submit the work and need help. JCDR provides authors help in this regards.
Timely publication of journal: Publication of manuscripts and bringing out the issue in time is one of the positive aspects of JCDR and is possible with strong support team in terms of peer reviewers, proof reading, language check, computer operators, etc. This is one of the great reasons for authors to submit their work with JCDR. Another best part of JCDR is "Online first Publications" facilities available for the authors. This facility not only provides the prompt publications of the manuscripts but at the same time also early availability of the manuscripts for the readers.
Indexation and online availability: Indexation transforms the journal in some sense from its local ownership to the worldwide professional community and to the public.JCDR is indexed with Embase & EMbiology, Google Scholar, Index Copernicus, Chemical Abstracts Service, Journal seek Database, Indian Science Abstracts, to name few of them. Manuscriptspublished in JCDR are available on major search engines ie; google, yahoo, msn.
In the era of fast growing newer technologies, and in computer and internet friendly environment the manuscripts preparation, submission, review, revision, etc and all can be done and checked with a click from all corer of the world, at any time. Of course there is always a scope for improvement in every field and none is perfect. To progress, one needs to identify the areas of one's weakness and to strengthen them.
It is well said that "happy beginning is half done" and it fits perfectly with JCDR. It has grown considerably and I feel it has already grown up from its infancy to adolescence, achieving the status of standard online e-journal form Indian continent since its inception in Feb 2007. This had been made possible due to the efforts and the hard work put in it. The way the JCDR is improving with every new volume, with good quality original manuscripts, makes it a quality journal for readers. I must thank and congratulate Dr Hemant Jain, Editor-in-Chief JCDR and his team for their sincere efforts, dedication, and determination for making JCDR a fast growing journal.
Every one of us: authors, reviewers, editors, and publisher are responsible for enhancing the stature of the journal. I wish for a great success for JCDR."



Thanking you
With sincere regards
Dr. Rajendra Kumar Ghritlaharey, M.S., M. Ch., FAIS
Associate Professor,
Department of Paediatric Surgery, Gandhi Medical College & Associated
Kamla Nehru & Hamidia Hospitals Bhopal, Madhya Pradesh 462 001 (India)
E-mail: drrajendrak1@rediffmail.com
On May 11,2011




Dr. Shankar P.R.

"On looking back through my Gmail archives after being requested by the journal to write a short editorial about my experiences of publishing with the Journal of Clinical and Diagnostic Research (JCDR), I came across an e-mail from Dr. Hemant Jain, Editor, in March 2007, which introduced the new electronic journal. The main features of the journal which were outlined in the e-mail were extensive author support, cash rewards, the peer review process, and other salient features of the journal.
Over a span of over four years, we (I and my colleagues) have published around 25 articles in the journal. In this editorial, I plan to briefly discuss my experiences of publishing with JCDR and the strengths of the journal and to finally address the areas for improvement.
My experiences of publishing with JCDR: Overall, my experiences of publishing withJCDR have been positive. The best point about the journal is that it responds to queries from the author. This may seem to be simple and not too much to ask for, but unfortunately, many journals in the subcontinent and from many developing countries do not respond or they respond with a long delay to the queries from the authors 1. The reasons could be many, including lack of optimal secretarial and other support. Another problem with many journals is the slowness of the review process. Editorial processing and peer review can take anywhere between a year to two years with some journals. Also, some journals do not keep the contributors informed about the progress of the review process. Due to the long review process, the articles can lose their relevance and topicality. A major benefit with JCDR is the timeliness and promptness of its response. In Dr Jain's e-mail which was sent to me in 2007, before the introduction of the Pre-publishing system, he had stated that he had received my submission and that he would get back to me within seven days and he did!
Most of the manuscripts are published within 3 to 4 months of their submission if they are found to be suitable after the review process. JCDR is published bimonthly and the accepted articles were usually published in the next issue. Recently, due to the increased volume of the submissions, the review process has become slower and it ?? Section can take from 4 to 6 months for the articles to be reviewed. The journal has an extensive author support system and it has recently introduced a paid expedited review process. The journal also mentions the average time for processing the manuscript under different submission systems - regular submission and expedited review.
Strengths of the journal: The journal has an online first facility in which the accepted manuscripts may be published on the website before being included in a regular issue of the journal. This cuts down the time between their acceptance and the publication. The journal is indexed in many databases, though not in PubMed. The editorial board should now take steps to index the journal in PubMed. The journal has a system of notifying readers through e-mail when a new issue is released. Also, the articles are available in both the HTML and the PDF formats. I especially like the new and colorful page format of the journal. Also, the access statistics of the articles are available. The prepublication and the manuscript tracking system are also helpful for the authors.
Areas for improvement: In certain cases, I felt that the peer review process of the manuscripts was not up to international standards and that it should be strengthened. Also, the number of manuscripts in an issue is high and it may be difficult for readers to go through all of them. The journal can consider tightening of the peer review process and increasing the quality standards for the acceptance of the manuscripts. I faced occasional problems with the online manuscript submission (Pre-publishing) system, which have to be addressed.
Overall, the publishing process with JCDR has been smooth, quick and relatively hassle free and I can recommend other authors to consider the journal as an outlet for their work."



Dr. P. Ravi Shankar
KIST Medical College, P.O. Box 14142, Kathmandu, Nepal.
E-mail: ravi.dr.shankar@gmail.com
On April 2011
Anuradha

Dear team JCDR, I would like to thank you for the very professional and polite service provided by everyone at JCDR. While i have been in the field of writing and editing for sometime, this has been my first attempt in publishing a scientific paper.Thank you for hand-holding me through the process.


Dr. Anuradha
E-mail: anuradha2nittur@gmail.com
On Jan 2020

Important Notice

Original article / research
Year : 2024 | Month : November | Volume : 18 | Issue : 11 | Page : AC01 - AC06 Full Version

Analysis of Ratio of Length of 2?supnd Digit to 4th Digit (2D:4D) among Transgender Women (MtF) and Cisgender People in South Indian Population: A Cross-sectional Study


Published: November 1, 2024 | DOI: https://doi.org/10.7860/JCDR/2024/74296.20338
Karthikeyan Annamalai, Deepti Shastri, Sathiya Subramaniam

1. Assistant Professor, Department of Anatomy, Annapoorana Medical College and Hospitals, Salem, Tamil Nadu, India. 2. Professor, Department of Anatomy, Vinayaka Mission's Kirupananda Variyar Medical College, Salem, Tamil Nadu, India. 3. Associate Professor, Department of Anatomy, Annapoorana Medical College and Hospitals, Salem, Tamil Nadu, India.

Correspondence Address :
Dr. Sathiya Subramaniam,
Associate Professor, Department of Anatomy, Annapoorana Medical College and Hospitals, NH-7 Shankari Main Road, Salem-636308, Tamil Nadu, India.
E-mail: sathiyasho@gmail.com

Abstract

Introduction: Transgender refers to an individual whose gender identity differs from the sex that was assigned at birth. Transgender women (MtF) were assigned male at birth but later recognised themselves as female. The length of the index finger (2D) in males is shorter than the length of the ring finger (4D), resulting in a low finger ratio due to higher androgen levels, whereas in females, the ratio is high due to low androgen levels. The index to ring finger length ratio in males and females is influenced by testosterone hormone levels during early foetal life, exhibiting sexual dimorphism. The 2D:4D ratio serves as one of the determinants for future generations to identify the transgender women population.

Aim: To determine the 2D:4D ratio among transgender women and the cisgender population.

Materials and Methods: A cross-sectional study was conducted in the Department of Anatomy, Annapoorana Medical College and Hospitals, Vinayaka Mission’s Medical College Hospitals, Salem, Tamil Nadu, India, from September 2018 to October 2019. A total of 392 adult participants between 19 years and 60 years of age were selected. The samples of transwomen were collected with the help of the President of Salem Thirunangaigal Nala Sangam (STNS) and Koovagam Koothandavar Temple in Tamil Nadu. Cisgender samples were collected from volunteers in Salem, Tamil Nadu. The 2D and 4D digit lengths of both hands were measured using Digimizer software with the help of images obtained by a Canon 220 photo scanner. Another type of classification was done by naked eye analysis without any calculations, based on which the 2D:4D ratio among males, females and transgender women was categorised into types 1, 2 and 3. The paired t-test was used to compare the male, female and transwomen populations.

Results: Out of 392 participants, 122 were cis females, 130 were cis males and 140 were transgender females. The p-value of the mean 2D:4D ratio was found to be significant in the right and left hands when comparing the 2D:4D ratio of males with females and transgender women with females. The p-value of the 2D:4D ratio for males with transgender women in the right and left hands was 0.451 and 0.943, respectively, which was statistically insignificant due to the high levels of androgen in these two groups.

Conclusion: The present study helps scientists in the gender study field to advance their knowledge about transgender individuals, which can fill the gaps in available information and overcome existing lacunae in this field.

Keywords

Androgen, Digit ratio, Index finger, Sexual dimorphism

Transgender studies have become increasingly prominent over the past several decades. The word “transgender” was coined by psychiatrist John F. Oliven of Columbia University in 1965. Transgender refers to an individual’s sense of personal identity that does not conform to their anatomical sex. For gender non conformity, the term transgender can be used. The various terms commonly used under the transgender umbrella are (FtM) for men and (MtF) for women, where individuals change themselves according to what they feel inside (1),(2). Cisgender identity and gender, however, match with the sex at birth. Prenatal androgen exposure in foetuses plays a vital role in determining the digit ratio. Male foetuses show lower (masculine) digit ratios due to more androgen exposure, whereas in female foetuses, the possibility of androgen exposure is less reflected as a higher (feminine) digit ratio (3). Males with a feminine digit ratio are more vulnerable to have low sperm counts, infertility and metabolic syndrome (4). The length of the index and ring finger (2D:4D) ratio varies in males and females as prenatal androgen exposure determines the digit length before birth (5). The 2D:4D ratio was found to be greater in females than males due to exposure to low androgen levels in utero, and the gender difference tends to be more for the right hand than the left hand as the majority of the population were right-handed individuals (6).

The present study not only helps scientists in transgender studies but also assists forensic experts in dealing with medicolegal cases to identify the gender of an unknown individual with residual body parts having only a detached upper limb by measuring the digit (2D:4D) ratio as it shows sexual dimorphism. Therefore the present study was aimed to determine gender differences in digit ratio (2D:4D) between transgender women and cisgender individuals in the South Indian population and to calculate the 2D:4D ratios, determine the percentage distribution among the three genders, and categorise them into types 1, 2 and 3.

Material and Methods

A cross-sectional study was conducted in the Department of Anatomy, Annapoorana Medical College and Hospitals, Vinayaka Mission’s Medical College and Hospitals, from September 2018 to October 2019. The Institutional Ethics Committee (IEC) granted ethical clearance for the present study (VMKVMC/IEC/18/62).

Sample size calculation: To calculate the sample size, reference values were available for males and females only; for transgender individuals, the reference values were not available. Therefore, the sample size was calculated based on the effect size. To calculate the sample size for three groups, the assumed medium effect, i.e., E=0.20, was used. The sample size was calculated using the software G Power version 3.1.9.2 with 90% power, resulting in 107 per group and a total of 321 (7). A non response rate of 15% was considered. Therefore, the final sample size of 126 per group and a total of 378 was used.

Inclusion criteria: Cisgender (both male and female) volunteers between 19 years and 60 years and government Certified adult Transgender women (MtF) between the age group 19 years and 60 years were included in the study.

Exclusion criteria: Other genderqueers like transmen (FtM), gay, lesbian, intersex and queer, and transwomen who did not give informed consent were excluded from the study.

Study Procedure

A total of 392 adult samples between 19 years and 60 years of age were selected. Samples from cisgender subjects were collected from Department of Anatomy, Annapoorana Medical College and Hospitals, Tamil nadu, India (cis females, n=50 and cis males, n=39), Vinayaka Mission’s Medical College and Hospitals, Tamil nadu, India (cis females, n=23 and cis males, n=31), and at Salem cis females, n=49 and cis males, n=60). All the samples of transwomen (n=140) were collected with the help of President STNS and Koovagam Koothandavar Temple in Tamil Nadu, India.

Subjects underwent multiple counselling sessions to help them understand the type of the research. Written informed consent was obtained from all interested participants. Out of 500 subjects approached, including cisgender and transgender women, only 392 subjects consented to participate.

Photo scanning image: All volunteers were asked to remove their ornamental jewellery from their fingers, and their hands were cleaned with soap and water to remove dirt. Once their hands were dry, a thin layer of non toxic white talcum powder was applied to the palms and fingers. The white powder enhances the ridges and creases of the hands for computer interpretation. A Canon CanoScan LiDe 220 photo scanner and a personal computer were used to record the image, ensuring that all fingers were straight and visible (A3). While scanning, a measurement scale, the subject’s name and ID number were placed next to the subject’s hand. Both the palmar sides, the subject’s name with ID number and the measurement scale were scanned simultaneously (A1A2). The images were standardised by setting a 10 mm length to 157 pixels (Table/Fig 1).

Software digimizer software: All the scanned images were labelled, converted to grayscale, and inverted to negative for better visualisation with the help of Photoshop 2018 software. Later, all the Joint Photographic Experts Group (JPEG) images were transferred to the Digimizer software for length measurements (Table/Fig 2) (8),(9).

The following measurements were taken on both the right and left hands as specified below (10):

• D2 (Index finger) length was measured between the midpoint of the basal crease and the fingertip.
• D4 (Ring finger) length was measured between the midpoint of the proximal basal crease and the fingertip.

Based on the lengths of the index and ring fingers, they are categorised into three visual types (11):

• Type 1: Length of the index finger longer than the ring finger.
• Type 2: Length of the index finger and ring finger equal.
• Type 3: Length of the ring finger longer than the index finger.

All the above measurements were taken thrice by two investigators, and the mean of the three values was considered as the final value. The readings were taken with 100% agreement by both observers.

Statistical Analysis

A detailed descriptive analysis was done by using Statistical Package for the Social Sciences (SPSS) software version 25.0. The mean, Standard Deviation (SD) and p-value were calculated and compared among cisgender males, cisgender females and transgender women. The paired t-test was used to compare the male, female and transgender women groups. The percentage distribution of each type based on the length of the index and ring fingers among the three genders was tabulated. A significance level of p-value<0.05 was considered significant.

Results

Out of 392 participants, 122 were cis females, 130 were cis males and 140 were transgender females (Table/Fig 3). The mean 2D:4D ratio in the right hand was comparatively greater than in the left hand in all three genders. The mean 2D:4D ratio of the right and left hands in females was higher than in the male and transgender women populations (Table/Fig 4).

The mean 2D:4D ratio between females and males shows a p-value of 0.010 on the right-side and a p-value of 0.001 on the left-hand side. A similar significant result was noted by comparing transgender women and female groups, showing a p-value of 0.001 in both the right and left hands. On the contrary, the mean 2D:4D ratio between transgender women and males showed a p-value of 0.451 on the right hand and a p-value of 0.943 on the left hand, confirming that there is no significant difference between these two groups, clarifying that transgender women’s somatic features are analogous to males (Table/Fig 5).

The percentage distribution of type 3 in males, females and transgender women on the right hand was 44.61%, 32.78%, and 46.43%, respectively, whereas on the left hand, it was 39.23%, 24.60%, and 42.86%, respectively. This implies that in males, the length of the ring finger was greater than the index finger, whereas in females, the length of the ring finger and index finger was the same (type 2) with 14.75% on the right hand and 18.03% on the left hand. Therefore, in males and transgender women, the type 3 finger pattern (Length of ring finger > Length of index finger) was more observed, whereas in females, the type 2 finger pattern was more prevalent than in males (Table/Fig 6).

The pair-wise comparison results show that the mean of females significantly differed from males and transgender women. However, the mean value of males did not differ significantly from transgender women (Table/Fig 7).

Discussion

The 2D:4D ratio in humans is of considerable interest in research as it exhibits sexual dimorphism. We analysed the 2D:4D ratio in relation to gender identification in the transgender population. The relative 2D:4D ratio might serve as a suitable indirect biomarker for prenatal androgen activity, influencing the masculinising effects on behaviour and secondary sexual characteristics of an individual.

In the present study, the mean 2D:4D ratio of the right hand in males, females and transgender women was found to be 0.9595, 0.974 and 0.9558, respectively. The mean 2D:4D ratio of the left hand in males, females and transgender women was found to be 0.943, 0.9618, and 0.9434, respectively.

A comparison was made between previous and present studies. Results of mean 2D:4D ratios among male, female and transgender women populations are shown in (Table/Fig 8) (11),(12),(13),(14),(15),(16),(17),(18),(19),(20),(21),(22),(23),(24),(25),(26).

Visual classification of index and ring finger: Robertson J et al., studied the 2D:4D ratio in males and females using hand radiographs. The mean 2D:4D ratio in males was less when compared to females and showed a significant difference with p-value<0.001. Based on finger length measurements, the hand was categorised into three types, and it was found that a majority of males fall under type 3 hand (ring finger greater than index finger). In females, type 3 was less in number since the index and ring finger lengths were almost similar, showing type 2. The male pattern 2D:4D finger type in males and transgender women was similar to the present study. However, in females, the percentage distribution of type 3 was less. The non gonadal somatic sex difference that masculinises males due to foetal androgen levels exhibits 2D:4D sexual dimorphism (11).

A 2D:4D ratio in the cisgender population: According to Van Hemmen J et al., Aboul-Hagag KE et al., and Gillam L et al., the mean 2D:4D ratio between females and males shows a significant p-value (<0.001) in both the right and left hand. It was found that the mean 2D:4D values were greater in females than in males, due to prenatal testosterone levels playing a vital role in digit length values, which correlates with the present study (12),(13),(14).

Canan F et al., conducted a study linking Problematic and Pathological Internet Use (PPIU) to 2D:4D ratios in a sample of both females and males, which shows a stronger association in males than in females due to prenatal testosterone concentration leading to addictive video gaming (15).

Dey S and Kapoor AK, and Jacob M et al., conducted a study in the Indian population, which revealed that the mean 2D:4D digit ratio in males (0.968) was significantly lower when compared to females (1.014). The sex differences were slightly different, higher for the right hand than the left hand, implying that the 2D:4D digit ratio in the right hand is more sensitive to foetal androgens than the left hand (16),(17).

Asuku A et al., reported that the mean 2D:4D ratio in males was less than in females, and the authors also noticed that the difference was more pronounced in the right hand than the left hand when comparing the urban with rural male and female populations. Certain environmental factors influence the digit ratio during the embryonic period, which may manifest in later life, establishing such a difference in urban-rural populations (18).

According to Jaiswal A et al., the mean 2D:4D ratio in males was lower compared to females due to prenatal testosterone levels, but no significant difference was observed in the 2D:4D ratio between the right and left hands. This comparison of the 2D:4D ratio among the cisgender group is in accordance with the present study (19).

Shokri H et al., compared the 2D:4D ratio between healthy males (n=72) and males with schizophrenia (n=62) and found that there was no significant difference (p-value>0.05) between the two groups except for the right index finger length. The index finger length serves as a biomarker to assess the prognosis of schizophrenia (20).

In a study on the 2D:4D ratio in the cisgender and transgender population, Hisasue S et al., described the mean 2D:4D ratio in gender identity disorder (FtM) as 0.955 and 0.954, in the right and left hand, which is lower when compared to female controls with 0.999 and 0.979, respectively in the right and left hand. This reflects early testosterone exposure in the foetus creating an impact on the 2D:4D ratio. Control males show a lower 2D:4D ratio than control females (21). The present study also shows a lower 2D:4D ratio in control males than in control females. The comparison between male and female transgender women (MtF) and females shows a significant p-value <0.001. However, the comparison between transgender women (MtF) and males showed a p-value >0.001, which implies that the concentration of sex hormones in early foetal life decides the architecture of the human body.

According to Leinung M and Wu C, the mean 2D:4D ratio for the dominant hand in transmen (FtM) was 0.983, which was lower than in female controls (0.998) but showed a similar ratio to male controls (0.972). No difference in the 2D:4D ratio was noted between transgender women (MtF) and male controls with a p-value of 0.434, which correlates with the present study. It concluded that the gender identity of transmen (FtM) was influenced by prenatal androgen exposure, whereas for transgender women (MtF), gender identity was influenced by additional factors like prenatal stress (22).

Siegmann EM et al., observed that the mean 2D:4D ratio in both the right and left hands in MtF individuals was higher than in male controls. However, the mean 2D:4D ratios in FtM individuals were lower than in female controls in both the right and left hands. No significant results were found when comparing FtM individuals with male controls in both the right and left hands. In contrast, when comparing MtF individuals with male controls, significance was noted only in the left hand (p-value=0.049), while it was insignificant in the right hand. In the present study, the comparison between MtF individuals and female controls was significant in both the right and left hands, but with male controls, it was not significant in either hand. The results slightly differ from those of the above-mentioned authors regarding the left hand in MtF individuals’ comparison with male controls since the 2D:4D ratio in the right hand is more sensitive to foetal androgens than the left hand, and prenatal androgen influences gender identity in individuals born as males (23).

Sadr M et al., compared the mean 2D:4D ratios of transgender women and transmen with a control group of the same natal sex. The results showed that transgender women had significantly less masculinised values compared to control males, while transmen had more masculinised values compared to control women. In the present study, the comparison between transgender women and control men showed similar results, with less masculinisation and more pronounced differences on the right hand. The results possibly predict weak prenatal testosterone effects in natal males, while strong prenatal testosterone effects in natal females may be a causative factor for gender dysphoria (24).

Saglam T et al., analysed the mean 2D:4D ratios of Assigned Female at Birth (AFB-GD) and Assigned Male at Birth (AMB-GD) with male and female controls. The mean 2D:4D ratio of AMB-GD did not differ significantly from the male controls in both the right and left hand. The finger ratio in the female controls shows a significant difference on the right hand (p-value<0.001), but the ratio was found to be insignificant on the left hand. The mean 2D:4D ratio of AFB-GD shows a significant difference (p-value=0.028) from the female controls in the right hand. However, there was an insignificant difference in the left hand. In the left hand, the ratio was insignificant with female controls and showed a significant difference (p-value=0.045) with male controls. The present study on transgender women shows a significant difference (p-value<0.001) with cis females in both the right and left hands. However, on the contrary, with cis males, the p-value (0.451, 0.943) was not significant on both the right and left hands, which correlates with Saglam T et al.’s findings as the prenatal exposure of the foetal brain to testosterone level determines gender identity (25).

Vujovic S et al., observed that the mean 2D:4D ratio of MtF with control males doesn’t show any significant difference, but values were similar to control females. However, the comparison between FtM with male and female controls shows the lowest ratio in the left hand. The present study correlates with Vujovic S et al.’s findings on transgender women, which show a significant difference with female controls in both the right and left hands. However, with male controls, it was not significant in both the right and left hands. The result describes that decreased androgen exposure in prenatal life may play a vital role in MtF gender identity (26).

A significant strength of the present study is the sample collection. Out of all genderqueers, selecting transwomen was a great challenge. The methodology of recording the 2D:4D ratio by a digital scanner is more accurate. It is a non invasive method that does not cause any harm to the participants. Further studies among transwomen and other genderqueer groups with a large sample size are needed to validate the findings of the present study. In the future, further research might explore a new standard methodology to robustly capture the dermatoglyphics pattern. In the present study, only the index and ring finger (2D:4D) ratio was studied. The study of other digits’ ratios could provide additional information that may possibly fulfill the lacunae.

Limitation(s)

The larger the sample size, the more precise the results will be. The authors focused solely on transwomen, making it challenging to include a greater number of samples. When recording the finger scanning of both hands, the accuracy may be influenced by the extent of finger spreading, potentially leading to a small error.

Conclusion

The 2D:4D ratio indirectly predicts the foetal testosterone to estradiol ratio, enlightening us to understand and explain different personality traits. The present study concluded that transgender women’s somatic appearance falls in favour of males based on the 2D:4D ratio, which needs further extended research on the dermatoglyphics of the transgender women population to endorse the current result. India lacks considerable data regarding third genders. In view of that, the present article provides one of the crucial anthropological measurements, which will be additive to digit ratio research in India as well as be useful for forensic research.

Author’s contribution: KA: Concept, designing, data collection, interpretation of results and execution of research. DS: Designing and interpretation of results. SS: Interpretation of results and manuscript preparation.

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DOI and Others

DOI: 10.7860/JCDR/2024/74296.20338

Date of Submission: Jul 16, 2024
Date of Peer Review: Aug 08, 2024
Date of Acceptance: Sep 11, 2024
Date of Publishing: Nov 01, 2024

AUTHOR DECLARATION:
• Financial or Other Competing Interests: None
• Was Ethics Committee Approval obtained for this study? Yes
• Was informed consent obtained from the subjects involved in the study? Yes
• For any images presented appropriate consent has been obtained from the subjects. Yes

PLAGIARISM CHECKING METHODS:
• Plagiarism X-checker: Jul 16, 2024
• Manual Googling: Aug 14, 2024
• iThenticate Software: Sep 10, 2024 (12%)

ETYMOLOGY: Author Origin

EMENDATIONS: 6

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