Diagnostic Efficacy of DNA Ploidy in Liquid Based Cervical Cytology using DNA Cytometry
Published: February 1, 2021 | DOI: https://doi.org/10.7860/JCDR/2021/45836.14478
Namrata P Awasthi, Sridhar Mishra, Akanksha Anand, Sarita Saxena, Nuzhat Husain
1. Additional Professor, Department of Pathology, Dr. Ram Manohar Lohia Institute of Medical Sciences, Lucknow, Uttar Pradesh, India.
2. Research Associate, Department of Pathology, Dr. Ram Manohar Lohia Institute of Medical Sciences, Lucknow, Uttar Pradesh, India.
3. Research Assistant, Klinikum Rechts Der Isar, TUM Munich, Bavaria, Germany.
4. Consultant, Department of Obstetrics and Gynaecology, Dr. Ram Manohar Lohia Institute of Medical Sciences, Lucknow, Uttar Pradesh, India.
5. Professor and Head, Department of Pathology, Dr. Ram Manohar Lohia Institute of Medical Sciences, Lucknow, Uttar Pradesh, India.
Correspondence Address :
Namrata P Awasthi,
Additional Professor, Department of Pathology, Ram Manohar Lohia Institute of
Medical Sciences, Vibhuti Khand, Gomti Nagar, Lucknow, Uttar Pradesh, India.
E-mail: namratapunit@yahoo.co.in
Abstract
Worldwide cervical cancer is the fourth most common cancer in women and high incidence is reported from India. Liquid Based Cytology (LBC) provides good morphology for detection of cellular abnormalities. We, therefore, reviewed diagnostic efficacy of conventional Pap staining, flow cytometry and Human Papilloma Virus (HPV) testing in cervical pre cancer and cancer. Narrative review of cervical pre cancer and cancer candidate biomarkers including Pap staining, HPV and flow cytometry from cervical cytology fluids, is based on a detailed review of the literature. Based on the so far conducted studies, a promising conclusion can be drawn, that cytometry when coupled with HPV DNA typing or the conventional cytology gives better results as compared to that of conventional cytology or DNA cytometry alone. Liquid cytology provides a good and stable source of cervical cells to carry out ploidy studies using DNA cytometry. The procedure should be used in conjunction with LBC and HPV detection.
Keywords
Flow cytometry, High grade squamous intraepithelial lesion, Human papilloma virus, Low grade squamous intraepithelial lesion, Squamous cell carcinoma