Year :
2021
| Month :
September
| Volume :
15
| Issue :
9
| Page :
EC01 - EC04
Full Version
Nuclear Morphometry as an Adjunct to Cytomorphology in the Diagnosis of Thyroid Lesions
Published: September 1, 2021 | DOI: https://doi.org/10.7860/JCDR/2021/49980.15331
Sabina Khan, Sayika Hameed, Mohd Jaseem Hasan, Musharraf Husain, Arun Sharma
1. Professor, Department of Pathology, Hamdard Institute of Medical Sciences and Research, New Delhi, India.
2. Resident, Department of Pathology, Hamdard Institute of Medical Sciences and Research, New Delhi, India.
3. Professor, Department of Pathology, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh, Utter Pradesh, India.
4. Professor, Department of Surgery, Hamdard Institute of Medical Sciences and Research, New Delhi, India.
5. Assistant Professor, Department of Ear Nose and Throat, Hamdard Institute of Medical Sciences and Research, New Delhi, India.
Correspondence Address :
Sabina Khan,
Flat No. 4, G Block, Jamia Hamdard Campus, New Delhi, India.
E-mail: drsabina1@gmail.com
Abstract
Introduction: Fine Needle Aspiration Cytology (FNAC) is a reliable and reproducible diagnostic technique for thyroid lesions. Recently, it has been suggested that evaluation of nuclear features may enhance the diagnostic utility of FNAC. However, the evaluation of nuclear morphometry is not well established in thyroid cytology.
Aim: To evaluate the role of nuclear morphometry in cytological evaluation of thyroid lesions.
Materials and Methods: This was a cross-sectional study conducted over a period from March 2019-February 2020 at Hamdard Institute of Medical Sciences and Research, New Delhi, India. Morphometry was done on 40 cases of thyroid aspirates which had histopathological concordance. Computerised nuclear morphometry was done by using photographs captured under Motic photomicrography system. Six parameters were measured- nuclear area, nuclear perimeter, minimal nuclear diameter, maximal nuclear diameter, nuclear compactness and LS ratio (Largest to Smallest dimension ratio). Data were entered in spreadsheet and then analysed using Statistical Package for Social Sciences (SPSS) version 20.0.
Results: Out of total 40 thyroid aspirates studied, included non neoplastic (19 cases), benign (12 cases) and malignant lesions (9 cases). All nuclear morphometry parameters comprising of nuclear area, nuclear perimeter, minimal nuclear diameter, maximal nuclear diameter showed an increasing trend from non neoplastic to benign to malignant with a statistically significant difference between benign and malignant groups (p-values <0.05) except for LS ratio and nuclear compactness.
Conclusion: Nuclear morphometry can aid in cytological diagnosis of thyroid lesions. If used judiciously, quantitative estimation of cytological nuclear features can be helpful in assessing thyroid lesions preoperatively thus complementing its cytomorphological features
Keywords
Aspiration cytology, Histopathological, Photomicrography, Thyroid aspirates
10.7860/JCDR/2021/49980.15331
Date of Submission: Apr 17, 2021
Date of Peer Review: May 25, 2021
Date of Acceptance: Aug 07, 2021
Date of Publishing: Sep 01, 2021
AUTHOR DECLARATION:
• Financial or Other Competing Interests: None
• Was Ethics Committee Approval obtained for this study? Yes
• Was informed consent obtained from the subjects involved in the study? Yes
• For any images presented appropriate consent has been obtained from the subjects. Yes
PLAGIARISM CHECKING METHODS:
• Plagiarism X-checker: Apr 23, 2021
• Manual Googling: Jul 08, 2021
• iThenticate Software: Aug 18, 2021 (22%)
ETYMOLOGY: Author Origin
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