Year :
2024
| Month :
June
| Volume :
18
| Issue :
6
| Page :
BK01 - BK04
Full Version
The Effect of Probiotic Supplementation on the Levels of Biomarkers in Diabetic Animal Model: A Research Protocol
Published: June 1, 2024 | DOI: https://doi.org/10.7860/JCDR/2024/69264.19474
Thiagarajan Sowmiya, Emmanuel Bhaskar, Veeraraghavan Gayathri, Parthasarathy Mohanalakshmi, Santhi Silambanan
1. PhD Scholar, Department of Biochemistry, Sri Ramachandra Institute of Higher Education and Research, Porur, Chennai, Tamil Nadu, India.
ORCID ID: 0000-0001-8908-9432
2. Professor and Unit Chief, Department of General Medicine, Sri Ramachandra Institute of Higher Education and Research, Porur, Chennai, Tamil Nadu, India.
ORCID ID:0000-0002-3524-641X
3. Head/Test Facility Management, Department of CEFTE, Sri Ramachandra Institute of Higher Education and Research, Porur, Chennai, Tamil Nadu, India.
ORCID ID:0000-0001-9764-0140
4. Professor and Head, Department of Biochemistry, Sri Muthukumaran Medical College Hospital and Research Institute, Chennai, Tamil Nadu, India.
ORCID ID:0009-0003-4917-5901
5. Professor, Department of Biochemistry, Sri Ramachandra Institute of Higher Education and Research, Porur, Chennai, Tamil Nadu, India.
ORCID ID:0000-0003-0720-6063
Correspondence Address :
Dr. Santhi Silambanan,
Professor, Department of Biochemistry, Sri Ramachandra Medical College and Research Institute, Sri Ramachandra Institute of Higher Education and Research (SRIHER) (DU), Chennai-600116, Tamil Nadu, India.
E-mail: santhisilsambanan@gmail.com
Abstract
Introduction: Type 2 Diabetes Mellitus (T2DM) is characterised by hyperglycaemia, insulin secretion defects, or resistance. Most T2DM drugs help to improve glycaemic status, but the response varies among individuals. The modern lifestyle with unhealthy eating habits leads to gut dysbiosis. Altered gut microbiota can disrupt the host’s metabolic and signaling pathways, intestinal barrier integrity, and function. Probiotics could restore a healthy microbiota in the intestine, thus improving glycaemic status.
Need of the study: Probiotics may assist in re-establishing a healthy microbiota composition in the intestine. Limited studies have evaluated the supplementation of probiotics for effectively managing T2DM.
Aim: To investigate the effects of probiotics on biomarker levels in type 2 diabetic male Wistar rats.
Materials and Methods: This interventional case-control study will be conducted at the Centre for Toxicology and Developmental Research (CEFTE) at Sri Ramachandra Institute of Higher Education and Research, Chennai, Tamil Nadu, India. The study will involve 46 male Wistar rats divided into five groups. Diabetes will be induced by feeding the animals a High-Fat Diet (HFD) and administering a low dose of Streptozotocin (STZ) injection. Groups 1 and 2 will be on basal and HFDs, serving as negative and positive controls, respectively. Groups 3, 4 and 5 will be the intervention groups. The study duration will be four weeks and three days for diabetes induction and six weeks for intervention. Blood samples will be collected periodically to assess biomarkers, and at the end of the study, internal organs will be harvested for histopathological examination. Institutional Ethics Committee (IEC) approval has been obtained. Categorical variables will be analysed using Chi-square or Fisher’s exact test, while continuous variables will be analysed using repeated measures Analysis of Variance (ANOVA). A p-value of ≤0.05 will be considered statistically significant, and statistical analysis will be performed using Statistical Package for Social Sciences (SPSS) version 16.0.
Keywords
Gut dysbiosis, Hyperglycaemia, Intestine, Microbiota
DOI: 10.7860/JCDR/2024/69264.19474
Date of Submission: Dec 25, 2023
Date of Peer Review: Jan 29, 2024
Date of Acceptance: May 01, 2024
Date of Publishing: Jun 01, 2024
AUTHOR DECLARATION:
• Financial or Other Competing Interests: The project is funded by the Sri Ramachandra Institute of Higher Education and Research through Founder-Chancellor Shri. N.P.V. Ramasamy Udayar Research Fellowship (Grant No. U02M210724).
• Was Ethics Committee Approval obtained for this study? Yes
• Was informed consent obtained from the subjects involved in the study? No
• For any images presented appropriate consent has been obtained from the subjects. No
PLAGIARISM CHECKING METHODS:
• Plagiarism X-checker: Dec 25, 2023
• Manual Googling: Feb 13, 2024
• iThenticate Software: Apr 29, 2024 (11%)
ETYMOLOGY: Author Origin
EMENDATIONS: 7
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