Buccal Micronuclei Assay as a Tool For Biomonitoring DNA Damage in Oral Lichen Planus
Published: July 1, 2016 | DOI: https://doi.org/10.7860/JCDR/2016/.8072
S. Vidyalakshmi, R. Madhavan Nirmal, V. Veeravarmal, A. Santhadevy, R. Aravindhan, Sumathy
1. Senior Lecturer, Department of Oral and Maxillofacial Pathology, Indira Gandhi Institute of Dental Sciences, Pillaiyarkuppam, Pondicherry, India.
2. Professor, Department of Oral and Maxillofacial Pathology, Rajah Muthiah Dental College and Hospital, Annamalai University, Chidambaram, India.
3. Professor, Department of Oral and Maxillofacial Pathology, Rajah Muthiah Dental College and Hospital, Annamalai University, Chidambaram, India.
4. Professor, Department of Oral and Maxillofacial Pathology, Indira Gandhi Institute of Dental Sciences, Pillaiyarkuppam, Pondicherry, India.
5. Senior Lecturer, Department of Oral and Maxillofacial Pathology, SRM Kattankulathur Dental College & Hospital, SRM University, Potheri, Kanchipuram dt, India.
6. Professor, Private Practioner, Pondicherry, India.
Correspondence Address :
Dr. R. Aravindhan,
Department of Oral and Maxillofacial Pathology, SRM Kattankulathur Dental College & Hospital,
SRM University, Potheri, Kanchipuram dt, India.
E-mail: araviopath86@gmail.com
Abstract
Introduction: The malignant transformation rate of Oral Lichen Planus (OLP) is between 0% and 5.8%. Oral lesions of lichen planus clinically presents itself multifocally, simulating the process of field cancerization in high risk malignancies. The Buccal MicroNucleus Cytome Assay (BMN Assay) provides a platform to identify the high risk individuals by evaluating the markers of nuclear damage at an earliest micro invasive phase.
Aim: To evaluate DNA damage in exfoliated buccal mucosal cells in individuals with oral lichen planus lesions and thereby to delineate the high risk group.
Materials and Methods: Buccal smears from 22 OLP and 10 control samples were stained in modified Feulgen-Rossenback reaction for micronuclei assay. Cytological evaluation of number of MicroNucleated cells (CMN), Total Number of Micronuclei (TMN) in micronucleated cells was done in both groups.
Results: Frequency of micronucleated cells (CMN) when compared among the study and control group, a mean value of 4.27 ± 1.80 and 0.90 ± 0.88 were obtained respectively. On comparing the total number of micronuclei in the micronucleated cells (TMN) between the study and control groups, a mean value of 5.38 ± 2.42 and 1.5 ± 0.88 were obtained respectively.
Conclusion: There was a significant increase in the frequency of micronuclei and the micronucleated cells in the oral lichen planus as compared to normal individuals.
Keywords
Biomarker, Exfoliative cytology, Feulgen-rossenback, Micronucleated cells