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Automated Triplex (HBV, HCV and HIV) NAT Assay Systems for Blood Screening in India
KE01-KE04
Correspondence
Dr. Manoj Kumar Rajput,
Scientist Grade – III, National Institute of Biologicals, Noida, India.
E-mail: mkrajput@hotmail.com
This review is confined to triplex nucleic acid testing (NAT) assays to be used on fully automated platform. Around the world, these assays are being used at various transfusion medicine centres or blood banks to screen blood units for HBV, HCV and HIV. These assay systems can screen up to 1000 blood units for HBV, HCV and HIV simultaneously in a day. This area has been dominated by mainly two manufacturers: M/s Gen-Probe-Novartis and M/s Roche Molecular Systems. The triplex NAT assay systems of both manufacturers are licensed by United States Food and Drug Administration. There is not much awareness about the technology and procedures used in these assays. The main objective of this review is to create awareness about the technology and procedure of these assays.