Detection of Porphyromonas gingivalis fimA Type I Genotype in Gingivitis by Real–Time PCR–A Pilot Study
ZC32-ZC35
Correspondence
Dr. Padma Krishnan,
Assistant Professor, Department of Microbiology, Dr. ALM PGIBMS, University of Madras, Chennai -600113, Tamilnadu, India.
E-mail: padma.abpkn@gmail.com
Introduction: Published literature till date reveals a high prevalence of Porphyromonas gingivalis fimA type I genotype among healthy subjects. Quite a few studies have reported its prevalence also in periodontitis patients. Nevertheless incidence of this genotype in gingivitis is lacking in adult population.
Aim: The present study was chosen to detect P. gingivalis fimA type I genotype among chronic gingivitis patients.
Materials and Methods: A total of 46 subgingival plaque samples collected from chronic marginal gingivitis (n=23) and chronic periodontitis subjects (control group) (n=23) were subjected to Real-Time Polymerase Chain Reaction to detect the P. gingivalis fimA type I gene. Statistical analysis was performed using chi-square test.
Results: Prevalence of P. gingivalis fimA type I gene among chronic periodontitis and chronic gingivitis patients were 8.7% and 30.4% respectively. P. gingivalis fimA type I genotype prevalence was found to be statistically insignificant between the two study groups (p=0.135).
Conclusion: The avirulent P. gingivalis fimA type I genotype, occurred in high prevalence among chronic gingivitis patients, while its presence was low in chronic periodontitis patients. Presence of this avirulent genotype in chronic marginal gingivitis signifies its reversible condition.