Erythrocyte Lipid Peroxidation, Glutathione, Ascorbic Acid, Vitamin E, Antioxidant Enzymes And Serum Homocysteine Levels In Patients With Coronary Artery Disease
Vishnu Priya V,Asst.Prof.,Dept.of Biochemistry,Saveetha Dental College&Hospital,Saveetha University,162,Poonamallee High Road,Chennaiâ€“600077,Tamilnadu,(INDIA).E-mail: firstname.lastname@example.org
Background: Coronary Artery Disease is the major cause of mortality and morbidity worldwide. It is associated with various risk factors such as age group (41 â€“ 60 years), male gender, smoking habit and hypertension. The exact pro-oxidant and antioxidant status in patients with Coronary Artery Disease is still not clear. To add a new insight to the question, changes in erythrocyte lipid peroxidation products (MDA), glutathione (GSH), ascorbic acid and plasma vitamin E, and activities of antioxidant enzymes like super oxide dismutase (SOD), glutathione peroxidase (GPX), catalase in erythrocytes, plasma glutathione â€“ S â€“ transferase (GST) and serum homocysteine levels were measured in patients with Coronary Artery Disease.
Aim: This work was undertaken to assess oxidative stress and antioxidant status in patients with Coronary Artery Disease and its contribution to the risk of cardiovascular disease.
Settings and Design: The study was conducted in sixty - five patients and the values were compared to control values. Erythrocyte MDA, GSH, ascorbic acid, plasma vitamin E and activities of antioxidant enzymes SOD, GPX, catalase in erythrocytes, plasma GST and serum homocysteine were estimated in Coronary Artery Disease patients. These parameters were measured in sixty - five patients and the values were compared to control values.
Statistical Analysis: Statistical analysis between group 1 (controls) and group 2 (patients) was performed by the Mann Whitney U test. The data was expressed as mean + SD. P < 0.05 was considered to be significant.
Results: It was observed that there was a significant increase in erythrocyte MDA levels, SOD, GPX and plasma GST activities, and a significant decrease in erythrocyte GSH, ascorbic acid, plasma vitamin E levels and catalase activity in patients with Coronary Artery Disease when compared to controls. Serum homocysteine levels were significantly higher in Coronary Artery Disease patients than in the controls.
Conclusions: The results of our study suggest higher oxygen free radical production which is evidenced by increased MDA and decreased GSH, ascorbic acid, vitamin E and Catalase activity and support to the oxidative stress in coronary artery disease. Increased homocysteine levels and decreased antioxidant capacity may contribute to the increased risk of cardiovascular disease in patients with coronary artery disease, in addition to known risk factors such as insulin resistance, hypertension, central obesity, and dyslipidaemia. The increased activities of antioxidant enzymes may be a compensatory regulation in response to increased oxidative stress.