NIDDM Antidiabetic Activity Of Saponins Of Momordica Cymbalaria In Streptozotocin-Nicotinamide NIDDM Mice 1460-1465
S. M. Firdous Mumtaz, Dept. of Pharmacology,
R R College of Pharmacy, Bangalore: 560090,
Karnataka, India. Phone 09886501454
E mail: firstname.lastname@example.org
Objective: To evaluate the Type 2 anti-diabetic activity of saponins obtained from Momordica Cymbalaria in Streptozotocinâ€“Nicotinamide Induced Type 2 diabetic mice.
Materials and Method: Type 2 diabetes in BALB/c mice was induced by a single intra-peritoneal injection of Streptozotocin (100 mg/ kg, i.p), 15 min after the intra-peritoneal administration of Nicotinamide (240 mg/ kg, i.p). Hyperglycaemia was confirmed by elevated blood glucose levels determined on day 7 after the injection. The saponin mixture was obtained from the ethanolic extract of Momordica Cymbalaria. Saponins of MC (SMC) 175mg/kg,p.o/30 days and Metformin350mg/kg,p.o/30 days were administered to the Type 2 diabetic mice. At the end of the treatment, their serum was analyzed for glucose, cholesterol, triglycerides and insulin; The pancreas of each mouse was studied to check whether there were any histological changes.
Result: Treatment of Type 2 diabetic mice with SMC (175mg/kg, p.o/30 days) and Metformin (350mg/kg,p.o/30 days) produced a significant fall in blood glucose (p<0.001), cholesterol (p<0.001), triglycerides (p<0.001) and an increase in the serum insulin level (p<0.001). Pancreatic islets and beta cells showed an increase in numbers.
Conclusion: Saponins of MC have significant Type 2 anti-diabetic activity and the activity may be due to increasing insulin secretion, probably by the regeneration of pancreatic beta cells.