Study Of The Hepatoprotective Activity Of The Ethanolic Extract Of The Pulp Of Eugenia Jambolana (Jamun) In Albino Rats 1466-1474
Gayatri Sarma, Dept of Pharmacology,
Assam Medical College & Hospital,
Dibrugarh - 786002. Ph : +919435043164.
Objective: To evaluate the hepatoprotective effect of the ethanolic extract of the pulp of Eugenia jambolana (EPEJ) on paracetamol (PCM)-induced hepatotoxicity in albino rats.
Materials and Methods: Healthy albino rats (thirty in number) of either sex, weighing 100-150 gms, were randomly divided into five groups of six animals each. Group A (Normal control) and Group B (Paracetamol-treated control) received 5ml/kg/day of 3% gum acacia; Groups C and D received the ethanolic extract of the pulp of Eugenia jambolana 100mg/kg/day and 200mg/kg/day respectively and Group E received silymarin 100mg/kg/day. All formulations were given orally for ten days. Hepatotoxicity was induced in Groups B, C, D and E by giving a single dose of paracetamol (2gm/kg) orally on the eighthth day of the experiment. Liver function tests (serum levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total bilirubin, total protein and albumin) and the histopathological examination (HPE) of the liver was done for all the five groups on the tenth day.
Statistical Analysis: One-way ANOVA, followed by Dunnettâ€™s multiple comparison test, were used for statistical analysis. Values of p < 0.01 were assumed to be significant.
Results: Group B showed a significant (p<0.01) increase in all serum marker enzymes and total bilirubin and a significant (p<0.01) decrease in total protein, as compared to Group A. In comparison to Group B, Groups C and D showed significant (p<0.01) reduction in the serum levels of all liver enzymes and total bilirubin and an increase in the total protein. HPE studies showed fatty changes, necrosis and fibrosis in Group B, while in Groups C, D and E, histopathology was near normal.
Conclusion: Thus, as revealed by the above study, the ethanolic extract of the pulp of Eugenia jambolana at 100 and 200mg/kg/day, possesses significant hepatoprotective activity in rats induced with hepatotoxin paracetamol.