Development Of A Micro Glucose Bulb For Plasma Glucose Assay In Two Drops Of Blood 3012-3014
Department of Biochemistry, Jawaharlal Nehru Medical College, Datta Meghe Institute of Medical Sciences, Wardha-442004, Maharashtra, (India)
We have devised a Micro-glucose bulb, where two drops of blood are collected by finger prick. After standing for 20 minutes, 10uL plasma can be collected by micropipette from bulb without centrifugation. The amount of plasma is sufficient for glucose estimation in 1000uL of glucose oxidase-peroxidase based reagent and absorbance can be read by a colorimeter.
This procedure of blood collection has been compared with that of classical procedure for glucose estimation. Regression analysis gave the equation of y = 1.008 + 0.248 and R value = 0.999. This validates the blood collection in Micro-glucose bulb.
We routinely employ this method of blood collection in our hospital when only glucose is to be estimated in a patient. We also use this method during screening of Diabetes mellitus in Community. This method will be useful to developing countries for time saving and economic reasons.