The Effect Of Hyperglycaemia On Some Biochemical Parameters In Diabetes Mellitus 3181-3186
Backside Civil Vet. Hospital,
Sikhan Wala Road, Prem Nagar,
P.O. Kotkapura -151204.
Distt. Faridkot, Punjab.
Diabetes Mellitus (DM) is the most common endocrinological disorder which is characterized by metabolic abnormalities and long term complications. The purpose of this study was to investigate the role of hyperglycaemia on various parameters like 1) Malondialdehyde (MDA), a parameter to study increased oxidative stress, as hyperglycaemia leads to increased lipid peroxidation 2) Adenosine Deaminase (ADA) activity which is suggested to be an important enzyme for modulating the bioactivity of insulin and 3) Free Fatty acids (FFA) to study dyslipidaemias which are associated with diabetes. Design and Methods: MDA, ADA and FFA levels in serum were measured spectrophotometrically in 50 patients of type 2 Diabetes Mellitus (DM) and also in 25 healthy controls. The patients were divided into three groups according to the levels of Glycosylated haemoglobin (HbA1c); Group I (with HbA1c 6-10%), Group II (with HbA1c 10-13%) and Group III (with HbA1c >13%). The results were also compared with the control group which had HbA1c of 4-6%. Results: All the three parameters, ADA, MDA and FFA levels were found to be significantly higher in the case subjects as compared to the controls (p<0.001). In addition to this correlation, the study revealed that MDA levels are positively associated with FBS and PPBS and also, that ADA is positively correlated with MDA (r = +0.57, p <0.001). Conclusion: These findings suggest that hyperglycaemia is associated with increased levels of ADA and that it is one of the factors which lead to the increased production of oxidative stress and also the derangements of lipid metabolism which are associated with DM. Also, ADA has got a role in increasing lipid peroxidation by reactive oxygen species (ROS) generation, as reflected by increasing MDA levels.