Auto Fluorescence And Fourier Transform – Infra Red (FTIR) Spectral Investigation On Di Ethyl Nitrosamine (DEN) Induced Hepatocellular Carcinoma, Treated With Pericarp Extract Of Garcinia Mangostana Linn In Rats 3289-3297
Surapaneni Krishna Mohan,
Department of Biochemistry,
Saveetha Medical College & Hospital,
Saveetha University, Saveetha Nagar,
Thandalam, Chennai â€“ 602 105, Tamilnadu, INDIA.
The aim of the present study was to analyze the tumour activity of diethyl nitrosamine (DEN); a chemical carcinogen which induced hepatocellular carcinoma in the liver tissue samples of male albino rats. The analysis has been carried out in the following conditions; normal, tumour induced and tumour treated liver tissue samples. In all these cases, the action of the diethyl nitrosamine drug in inducing tumours in the liver has been investigated. Garcinia mangostana pericarp extract was the Ayurvedic drug which was chosen for the treatment of hepatocellular carcinoma. The control liver tissues, those in which tumour formation was induced by using diethyl nitrosamine and those in which the tumour was treated with the Garcinia mangostana pericarp extract after inducing tumor formation were analyzed by using auto fluorescence spectroscopy and fourier transform infra red (FTIR) spectroscopy. The qualitative spectral analysis was done by using auto fluorescence spectroscopy and the quantitative study was carried out by using FTIR spectroscopy. The tissue samples were experimented with auto fluorescence spectroscopy at the excitation wavelengths of 280nm, 325nm and 405nm, which exhibits emission due to tryptophan, collagen and porphyrin, respectively. It was observed that in all the tissue samples, the effect of diethylnitrosamine in inducing tumours was predominant. However, on treating the tumours with the pericarp extract of Garcinia mangostana, they responded positively. FTIR spectra have been recorded in the mid frequency region of 4000 â€“ 450 cm-1 for all the liver tissue samples. The samples were analyzed quantitatively by using intensity ratio calculation among the selected absorption peaks to study the biochemical changes in the tissue samples. From this study, it was found that the total protein content was decreased in the liver tissues after inducing them with diethyl nitrosamine, thus causing the hepato cellular carcinoma. During the recovery phase, the decreased levels of the bio-chemical constituents were restored to near normal levels.