Studies on the in Vitro Anti-Oxidant
Properties of a Polyherbal Formulation:
Dr. K.R Subash
Assistant Professor, Department of Pharmacology,
Meenakshi Medical College & Research Institute,
MAHER(Meenakshi University), Kanchipuram,
PIN â€“ 631552 India.
The Polyherbal Formulation (PHF), Rumalaya Forte that has a combination of medicinal herbs such as the powders of Boswellia serrata, Commiphora wightii, Alpinia galanga and Glycyrrhiza glabra and the extracts of Tribulus terrestris and Tinospora cordifolia, was tested for its antioxidant and free radical scavenging activity in vitro. This PHF has been traditionally used by the ayurvedic practioners in India, for the treatment of various inflammatory disorders. The in vitro scavenging of the NO radical activity, Lipid Peroxidation (LPO) inhibition and the 1-Diphenyl-2-Picrylhydrazil (DPPH) inhibition of PHF were tested by using a spectrophotometer at absorbance levels of 546nm, 532 and 517nm respectively. The experiments were performed in triplicates with different concentrations of PHF (1.95-500 g/ml).The percentage inhibition IC50 values were 203.57%, 315.92% and 10.37% g/ml for LPO, DPPH and NO respectively. The polyherbal formulation, Rumalaya Forte demonstrated a significant NO free radical scavenging activity.