Histochemical Study of Inflammatory Lesions of Gall Bladder with Reference to Metaplastic Conditions EC01-EC04
Dr. Anadi Roy Chowdhury,
1, Khudiram Bose Sarani, Kolkata-700004, West Bengal, India.
Introduction: Cholelithiasis and asymptomatic gallstones may produce pathological changes like hyperplasia and metaplasia of the gall bladder mucosal epithelium. It has been suggested that metaplastic epithelium is more susceptible to malignant transformation than the normal mucosa and the intestinal metaplasia-dysplasia-carcinoma sequence exists in the gall bladder. So, it is essential to detect metaplastic lesions of gall bladder early and mucin histochemistry study may be useful in this regard.
Aim: To find out if mucin histochemistry can be used for early detection of metaplastic lesions of gall bladder as metaplastic epithelium is more prone to malignant transformation.
Materials and Methods: A total of 1153 formalin-fixed paraffin-embedded surgically resected gallbladder tissue samples were sectioned. All sections were stained with Haematoxylin-Eosin (HE), Alcian Blue (AB) and Periodic Acid-Schiff (PAS) stain.
Results: A total of 1153 cholecystectomy cases were observed. Maximum cases were of chronic cholecystitis with gall stones (841 cases). Metaplasia was found in 201 out of 1153 cases. Mucin histochemistry in metaplastic group showed only PAS positivity in 52 cases (neutral mucin), both PAS and AB positivity in 122 cases (acidic mucin) and only AB positivity in 27 cases (acidic mucin).
Conclusion: The gastric and intestinal metaplasia showed positivity more with acidic mucin than neutral mucin. Study of mucin histochemistry in metaplastic cases can lead to early detection and early diagnosis of carcinoma gallbladder.